LOCUS       sequence-177516-         595 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 3 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc3-HISNDHFR
FEATURES             Location/Qualifiers
     source          1..595
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(475..495)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(523..540)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             560..582
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 acgctcgagg atttcgaaac aatagctatg cgagttctgc gcatcagcat cgtggaattc
       61 gctgaatacc gattcccagt catccggctc gtaatccgga aaatgggtgt cgccttccac
      121 ttctgcatcg atatgcgtca gatacagttt ttgcgctttt ggcaagaact gttcataaac
      181 gcgtccgccg ccaatcacca tgatttctgg tacgtcacca cacgccgcga tggcttcgtc
      241 gaccgattta acccaggtaa cccgatcatc ggtcccgggt tgactgctga ggataatatt
      301 tttgcggccg ggcaaaggcc taccgattga ttcccaggta tggcgcccca taatcacggg
      361 tttatttaag gtgttgcgtt taaaccaggc gagatcggca ggcaggttcc atggcatggc
      421 gttttccatg ccgataacgc gatctaccgc tagcgccgca atcagactga tggatccctg
      481 gaagtacagg ttttcaccac caccaccacc agatccgctg ctgtgatgat gatgatgatg
      541 gctgctgccc atatgtatat ctccttctta aagttaaaca aaattattga gctct
//
