LOCUS       sequence-177519-         587 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 3 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc3-FLAGNDHFR
FEATURES             Location/Qualifiers
     source          1..587
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(468..488)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(510..533)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             547..569
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aggatttcga aacaatagct atgcgagttc tgcgcatcag catcgtggaa ttcgctgaat
       61 accgattccc agtcatccgg ctcgtaatcc ggaaaatggg tgtcgccttc cacttctgca
      121 tcgatatgcg tcagatacag tttttgcgct tttggcaaga actgttcata aacgcgtccg
      181 ccgccaatca ccatgatttc tggtacgtca ccacacgccg cgatggcttc gtcgaccgat
      241 ttaacccagg taacccgatc atcggtcccg ggttgactgc tgaggataat atttttgcgg
      301 ccgggcaaag gcctaccgat tgattcccag gtatggcgcc ccataatcac gggtttattt
      361 aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccatggcat ggcgttttcc
      421 atgccgataa cgcgatctac cgctagcgcc gcaatcagac tgatggatcc ctggaagtac
      481 aggttttcac caccaccacc accagatccc ttgtcatcat cgtccttgta gtctcccata
      541 tgtatatctc cttcttaaag ttaaacaaaa ttattgagct ctccgga
//
