LOCUS       sequence-17752-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Storz P, Toker A
  TITLE     Protein kinase D mediates a stress-induced NF-kappaB activation and 
            survival pathway.
  JOURNAL   EMBO J. 2003 Jan 2. 22(1):109-20.
  PUBMED    12505989
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2003 Jan 2. 22(1):109-20."
COMMENT     Sequence Label: pSUPER.PKD1.RNAi
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        21..235
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     156..175
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(308..324)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(309..325)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(353..371)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(392..408)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(392..408)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(405..427)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    416..432
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(440..446,447..464,465..470))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    485..506
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(623..640)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cccccgggct gcaggaattc gaacgctgac gtcatcaacc cgctccaagg aatcgcgggc
       61 ccagtgtcac taggcgggaa cacccagcgc gcgtgcgccc tggcaggaag atggctgtga
      121 gggacagggg agtggcgccc tgcaatattt gcatgtcgct atgtgttctg ggaaatcacc
      181 ataaacgtga aatgtctttg gatttgggaa tcttataagt tctgtatgag accacagatc
      241 cccatgctgt gggggctggt acttcaagag agtaccagcc cccacagcat tttttggaaa
      301 agcttatcga taccgtcgac ctcgaggggg ggcccggtac cagcttttgt tccctttagt
      361 gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      421 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      481 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      541 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      601 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      661 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      721 acgcaggaaa gaacatgtga gcaaaaggcc
//
