LOCUS       sequence-177523-        5781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization by Super-Resolution microscopy of beta-Actin fused to 
            mEOS3.1 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5781)
  TITLE     Unpublished Henriques Super-Resolution Microscopy Plasmids
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RHPMP132 (mEOS3.1-beta Actin)
FEATURES             Location/Qualifiers
     source          1..5781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..354
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        355..558
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     509..529
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             600..1277
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.1
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2559..2680
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2596..2615)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2650..2669
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2687..3142)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2824..2845)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3036..3055
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3169..3273
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3275..3632
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3275..3466
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3298..3318)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3483..3618
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3667..4461
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3667..4461
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3721..3740)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4331..4350
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4649..4668)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4693..4740
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5069..5657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5558..5577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
       61 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      121 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      181 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      241 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      301 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      361 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      421 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      481 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      541 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcggtc gccgccacca
      601 tgagtgcgat taagccagac atgaagatca aactccgtat ggaaggcaac gtaaacgggc
      661 accactttgt gatcgacgga gatggtacag gcaagccttt tgagggaaaa cagagtatgg
      721 atcttgaagt caaagagggc ggacctctgc cttttgcctt tgatatcctg accactgcat
      781 tccattacgg caacagggta ttcgccaaat atccagacaa catacaagac tattttaagc
      841 agtcgtttcc taaggggtat tcgtgggaac gaagcttgac tttcgaagac gggggcattt
      901 gcaacgccag aaacgacata acaatggaag gggacacttt ctataataaa gttcgatttt
      961 atggtaccaa ctttcccgcc aatggtccag ttatgcagaa gaagacgctg aaatgggagc
     1021 cctccactga gaaaatgtat gtgcgtgatg gagtgctgac gggtgatgta gagatggctt
     1081 tgttgcttga aggaaatgcc cattaccgat gtgacttcag aactacttac aaagctaagg
     1141 agaagggtgt caagttacca ggcgcccact ttgtggacca ctgcattgag attttaagcc
     1201 atgacaaaga ttacaacaag gttaagctgt atgagcatgc tgttgctcat tctggattgc
     1261 ctgacaatgc cagacgatcc ggactagatc tcgatcgagc tatggatgat gatatcgccg
     1321 cgctcgtcgt cgacaacggc tccggcatgt gcaaggccgg cttcgcgggc gacgatgccc
     1381 cccgggccgt cttcccctcc atcgtggggc gccccaggca ccagggcgtg atggtgggca
     1441 tgggtcagaa ggattcctat gtgggcgacg aggcccagag caagagaggc atcctcaccc
     1501 tgaagtaccc catcgagcac ggcatcgtca ccaactggga cgacatggag aaaatctggc
     1561 accacacctt ctacaatgag ctgcgtgtgg ctcccgagga gcaccccgtg ctgctgaccg
     1621 aggcccccct gaaccccaag gccaaccgcg agaagatgac ccagatcatg tttgagacct
     1681 tcaacacccc agccatgtac gttgctatcc aggctgtgct atccctgtac gcctctggcc
     1741 gtaccactgg catcgtgatg gactccggtg acggggtcac ccacactgtg cccatctacg
     1801 aggggtatgc cctcccccat gccatcctgc gtctggacct ggctggccgg gacctgactg
     1861 actacctcat gaagatcctc accgagcgcg gctacagctt caccaccacg gccgagcggg
     1921 aaatcgtgcg tgacattaag gagaagctgt gctacgtcgc cctggacttc gagcaagaga
     1981 tggccacggc tgcttccagc tcctccctgg agaagagcta cgagctgcct gacggccagg
     2041 tcatcaccat tggcaatgag cggttccgct gccctgaggc actcttccag ccttccttcc
     2101 tgggcatgga gtcctgtggc atccacgaaa ctaccttcaa ctccatcatg aagtgtgacg
     2161 tggacatccg caaagacctg tacgccaaca cagtgctgtc tggcggcacc accatgtacc
     2221 ctggcattgc cgacaggatg cagaaggaga tcactgccct ggcacccagc acaatgaaga
     2281 tcaagatcat tgctcctcct gagcgcaagt actccgtgtg gatcggcggc tccatcctgg
     2341 cctcgctgtc caccttccag cagatgtgga tcagcaagca ggagtatgac gagtccggcc
     2401 cctccatcgt ccaccgcaaa tgcttctagg gatccaccgg atctagataa ctgatcataa
     2461 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     2521 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     2581 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     2641 attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt
     2701 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     2761 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     2821 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     2881 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     2941 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     3001 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     3061 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     3121 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     3181 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3241 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga
     3301 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3361 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     3421 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     3481 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     3541 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     3601 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
     3661 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     3721 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     3781 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     3841 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     3901 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     3961 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     4021 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     4081 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     4141 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
     4201 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     4261 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     4321 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     4381 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     4441 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     4501 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     4561 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     4621 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     4681 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
     4741 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     4801 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     4861 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
     4921 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4981 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5041 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5101 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5161 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5221 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5281 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5341 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5401 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5461 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5521 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5581 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5641 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5701 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5761 ccgccatgca ttagttatta a
//
