LOCUS       sequence-177526-         612 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 3 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc3-STRHISNDHFR
FEATURES             Location/Qualifiers
     source          1..612
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(471..491)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(519..536)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(546..569)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     RBS             586..608
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tcgaggattt cgaaacaata gctatgcgag ttctgcgcat cagcatcgtg gaattcgctg
       61 aataccgatt cccagtcatc cggctcgtaa tccggaaaat gggtgtcgcc ttccacttct
      121 gcatcgatat gcgtcagata cagtttttgc gcttttggca agaactgttc ataaacgcgt
      181 ccgccgccaa tcaccatgat ttctggtacg tcaccacacg ccgcgatggc ttcgtcgacc
      241 gatttaaccc aggtaacccg atcatcggtc ccgggttgac tgctgaggat aatatttttg
      301 cggccgggca aaggcctacc gattgattcc caggtatggc gccccataat cacgggttta
      361 tttaaggtgt tgcgtttaaa ccaggcgaga tcggcaggca ggttccatgg catggcgttt
      421 tccatgccga taacgcgatc taccgctagc gccgcaatca gactgatgga tccctggaag
      481 tacaggtttt caccaccacc accaccagat ccgctgctgt gatgatgatg atgatggcta
      541 gatcctttct cgaactgcgg gtggctccag cttgccatat gtatatctcc ttcttaaagt
      601 taaacaaaat ta
//
