LOCUS       sequence-177532-         524 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 3 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc3-FLAGHISNDHFR
FEATURES             Location/Qualifiers
     source          1..524
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(422..442)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(470..487)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(497..520)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggaattcgct gaataccgat tcccagtcat ccggctcgta atccggaaaa tgggtgtcgc
       61 cttccacttc tgcatcgata tgcgtcagat acagtttttg cgcttttggc aagaactgtt
      121 cataaacgcg tccgccgcca atcaccatga tttctggtac gtcaccacac gccgcgatgg
      181 cttcgtcgac cgatttaacc caggtaaccc gatcatcggt cccgggttga ctgctgagga
      241 taatattttt gcggccgggc aaaggcctac cgattgattc ccaggtatgg cgccccataa
      301 tcacgggttt atttaaggtg ttgcgtttaa accaggcgag atcggcaggc aggttccatg
      361 gcatggcgtt ttccatgccg ataacgcgat ctaccgctag cgccgcaatc agactgatgg
      421 atccctggaa gtacaggttt tcaccaccac caccaccaga tccgctgctg tgatgatgat
      481 gatgatggct agatcccttg tcatcatcgt ccttgtagtc tccc
//
