LOCUS       sequence-177538-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 3 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as 
            positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc3-DHFRSTR
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             530..553
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(631..649)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      645..692
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatggga tccatcagtc tgattgcggc
       61 gctagcggta gatcgcgtta tcggcatgga aaacgccatg ccatggaacc tgcctgccga
      121 tctcgcctgg tttaaacgca acaccttaaa taaacccgtg attatggggc gccatacctg
      181 ggaatcaatc ggtaggcctt tgcccggccg caaaaatatt atcctcagca gtcaacccgg
      241 gaccgatgat cgggttacct gggttaaatc ggtcgacgaa gccatcgcgg cgtgtggtga
      301 cgtaccagaa atcatggtga ttggcggcgg acgcgtttat gaacagttct tgccaaaagc
      361 gcaaaaactg tatctgacgc atatcgatgc agaagtggaa ggcgacaccc attttccgga
      421 ttacgagccg gatgactggg aatcggtatt cagcgaattc cacgatgctg atgcgcagaa
      481 ctcgcatagc tattgtttcg aaatcctcga gcgtcgtgcc ggcagcgctt ggagccaccc
      541 gcagttcgaa aaataatgta caggtaccac gcgtgcgcgc tgatccggct gctaacaaag
      601 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      661 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tcgggattag
      721 cccgcctaat gagcgggctt ttttttaatt cccctatttg tttatttttc taaatacatt
      781 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaa
//
