LOCUS       sequence-177539-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-HISDHFR
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(491..508)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             528..550
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(587..606)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(588..606)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cggcacgacg ctcgaggatt tcgaaacaat agctatgcga gttctgcgca tcagcatcgt
       61 ggaattcgct gaataccgat tcccagtcat ccggctcgta atccggaaaa tgggtgtcgc
      121 cttccacttc tgcatcgata tgcgtcagat acagtttttg cgcttttggc aagaactgtt
      181 cataaacgcg tccgccgcca atcaccatga tttctggtac gtcaccacac gccgcgatgg
      241 cttcgtcgac cgatttaacc caggtaaccc gatcatcggt cccgggttga ctgctgagga
      301 taatattttt gcggccgggc aaaggcctac cgattgattc ccaggtatgg cgccccataa
      361 tcacgggttt atttaaggtg ttgcgtttaa accaggcgag atcggcaggc aggttccatg
      421 gcatggcgtt ttccatgccg ataacgcgat ctaccgctag cgccgcaatc agactgatgg
      481 atccgctgct gtgatgatga tgatgatggc tgctgcccat atgtatatct ccttcttaaa
      541 gttaaacaaa attatttccg gagccggagg gaaaccgttg tggtctccct atagtgagtc
      601 gtattaattt cgcgggatcg atgcatggtg ccaacaccta catctgtatt aacgaagcgc
      661 tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta
      721 ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc
      781 ctctctcgtt tcatcggtat cattacccca tgaacagaaa tcccccttac acggaggcat
      841 cagtgaccaa acaggaaaaa accgccctta acatggccc
//
