LOCUS       sequence-177541-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-FLAGDHFR
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(519..542)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             556..578
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(615..634)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(616..634)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cagccggatc agcgcgcacg cgttgtacat tagccggcac gacgctcgag gatttcgaaa
       61 caatagctat gcgagttctg cgcatcagca tcgtggaatt cgctgaatac cgattcccag
      121 tcatccggct cgtaatccgg aaaatgggtg tcgccttcca cttctgcatc gatatgcgtc
      181 agatacagtt tttgcgcttt tggcaagaac tgttcataaa cgcgtccgcc gccaatcacc
      241 atgatttctg gtacgtcacc acacgccgcg atggcttcgt cgaccgattt aacccaggta
      301 acccgatcat cggtcccggg ttgactgctg aggataatat ttttgcggcc gggcaaaggc
      361 ctaccgattg attcccaggt atggcgcccc ataatcacgg gtttatttaa ggtgttgcgt
      421 ttaaaccagg cgagatcggc aggcaggttc catggcatgg cgttttccat gccgataacg
      481 cgatctaccg ctagcgccgc aatcagactg atggatccct tgtcatcatc gtccttgtag
      541 tctcccatat gtatatctcc ttcttaaagt taaacaaaat tatttccgga gccggaggga
      601 aaccgttgtg gtctccctat agtgagtcgt attaatttcg cgggatcgat gcatggtgcc
      661 aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc
      721 ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc
      781 atcatcagta acccgtatcg tgagcatcct ctctcgttt
//
