LOCUS       sequence-177543-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-HISNDHFR
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(466..486)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(514..531)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             551..573
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(610..629)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(611..629)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gatttcgaaa caatagctat gcgagttctg cgcatcagca tcgtggaatt cgctgaatac
       61 cgattcccag tcatccggct cgtaatccgg aaaatgggtg tcgccttcca cttctgcatc
      121 gatatgcgtc agatacagtt tttgcgcttt tggcaagaac tgttcataaa cgcgtccgcc
      181 gccaatcacc atgatttctg gtacgtcacc acacgccgcg atggcttcgt cgaccgattt
      241 aacccaggta acccgatcat cggtcccggg ttgactgctg aggataatat ttttgcggcc
      301 gggcaaaggc ctaccgattg attcccaggt atggcgcccc ataatcacgg gtttatttaa
      361 ggtgttgcgt ttaaaccagg cgagatcggc aggcaggttc catggcatgg cgttttccat
      421 gccgataacg cgatctaccg ctagcgccgc aatcagactg atggatccct ggaagtacag
      481 gttttcacca ccaccaccac cagatccgct gctgtgatga tgatgatgat ggctgctgcc
      541 catatgtata tctccttctt aaagttaaac aaaattattt ccggagccgg agggaaaccg
      601 ttgtggtctc cctatagtga gtcgtattaa tttcgcggga tcgatgcatg gtgccaacac
      661 ctacatctgt attaacga
//
