LOCUS       sequence-177544-        4139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Streptococci shuttle plasmid for gene expression in 
            streptococci with P23 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Biswas I, Jha JK, Fromm N
  TITLE     Shuttle expression plasmids for genetic studies in Streptococcus 
            mutans.
  JOURNAL   Microbiology. 2008 Aug;154(Pt 8):2275-82. doi: 
            10.1099/mic.0.2008/019265-0.
  PUBMED    18667560
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology."; date: "2008-08"; volume: "154(Pt 8):2275-82. doi"; 
            pages: " 10.1099/mic.0.2008/019265-0"
COMMENT     Sequence Label: pIB165
FEATURES             Location/Qualifiers
     source          1..4139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2952..2963
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4006..4023
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4111..4134
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 agcttaagtg attagtcaaa gaatggtgat gacaattgta caggttatca ataaagccat
       61 tgatactctc attcttatcc cgtatttatt aacttctcct agtattagta attatcatgg
      121 ctgtcatggc gcattaacgg aataaagggt gtgcttaaat cgggccattt tgcgtaataa
      181 gaaaaaggat taattatgag cgaattgaat taataataag gtaatagatt tacattagaa
      241 aatgaaaggg gattttatgc gtgagaatgt tacagtctat cccggcaata gttaccctta
      301 ttatcaagat aagaaagaaa aggatttttc gctacgctca aatcctttaa aaaaacacaa
      361 aagaccacat tttttaatgt ggtcttttat tcttcaacta aagcacccat tagttcaaca
      421 aacgaaaatt ggataaagtg ggatattttt aaaatatata tttatgttac agtaatattg
      481 acttttaaaa aaggattgat tataatgaag aaagcagaca agtaagcctc ctaaattcac
      541 tttagataaa aatttaggag gcatatcaaa tgaactttaa taaaattcac tttagataaa
      601 aatttaggag gcatatcaaa tgaactttaa taaaattgat ttagacaatt ggaagagaaa
      661 agagatattt aatcattatt tgaaccaaca aacgactttt agtataacca cagaaattga
      721 tattagtgtt ttataccgaa acataaaaca agaaggatat aaattttacc ctgcatttat
      781 tttcttagtg acaagggtga taaactcaaa tacagctttt agaactggtt acaatagcga
      841 cggagagtta ggttattggg ataagttaga gccactttat acaatttttg atggtgtatc
      901 taaaacattc tctggtattt ggactcctgt aaagaatgac ttcaaagagt tttatgattt
      961 atacctttct gatgtagaga aatataatgg ttcggggaaa ttgtttccca aaacacctat
     1021 acctgaaaat gctttttctc tttctattat tccatggact tcatttactg ggtttaactt
     1081 aaatatcaat aataatagta attaccttct acccattatt acagcaggaa aattcattaa
     1141 taaaggtaat tcaatatatt taccgctatc tttacaggta catcattctg tttgtgatgg
     1201 ttatcatgca ggattgttta tgaactctat tcaggaattg tcagataggc ctaatgactg
     1261 gcttttataa tatgagataa tgccgactgt actttttaca gtcggttttc taatgtcact
     1321 aacctgcccc gttagttgaa gaaggttttt atattacagc tccagatcca tatccttctt
     1381 tttctgaacc gacttctcct ttttcgcttc tttattccaa ttgctttatt gacgttgagc
     1441 ctcggaaccc ttaacaatcc caaaacttgt cgactttcgt caggggggct tttatttatt
     1501 caataatccc tcctctcaat aaatctattg ttgtacttaa ttcaacttcc atttctctgt
     1561 atctttcaat acgctctttt aagtccttaa tttctttttt taattcctca ttttcagcaa
     1621 ataactcttt ttctttgttt gtcattttat ttcccccgtt tcagcatcaa gaacctttgc
     1681 ataacttgct ctatatccac actgataatt gccctcaaac cataatctaa aggcgctaga
     1741 gtttgttgaa acaatatctt ttacatcatt cgtatttaaa attccaaact ccgctcccct
     1801 aaggcgaata aaagccatta actcttttgt atttaccaaa ttatagtcat ccactatatc
     1861 taaaagtaaa ttcttcaatt ttcttttttg gctttcatca agtgttatat agcggtcaat
     1921 atcaaaatca ttaatgttca aaatatcttt tttgtcgtat atatgtttat tcttagcaat
     1981 agcgtccttt gattcatgag tcaaatattc atatgaacct ttgatataat caagtatctc
     2041 aacatgagca actgaactat tccccaattt tcgcttaatc ttgttcctaa cgctttctat
     2101 tgttacagga tttcgtgcaa tatatataac gtgatagtgt ggttttttat agtgctttcc
     2161 atttcgtata acatcactac tattccatgt atctttatct tttttttcgt ccatatcgtg
     2221 taaaggactg acagccatag atacgcccaa actctctaat ttttctttcc aatcattagg
     2281 aattgagtca ggatataata aaaatccaaa atttctagct ttagtatttt taatagccat
     2341 gatataatta ccttatcaaa aacaagtagc gaaaactcgt atccttctaa aaacgcgagc
     2401 tttcgcttat tttttttgtt ctgattcctt tcttgcatat tcttctatag ctaacgccgc
     2461 aaccgcagat tttgaaaaac ctttttgttt cgccatatct gttaattttt tatcttgctc
     2521 ttttgtcaga gaaatcataa ctcttttttt cgattctgaa atcaccattt aaaaaactcc
     2581 aatcaaataa ttttataaaa ttagtgtatc actttgtaat cataaaaaca acaataaagc
     2641 tacttaaata tagatttata aaaaacgttg gcgaaaacgt tggcgattcg ttggcgattg
     2701 aaaaacccct caaacccttg agccagttgg gatagagcgt ttttggcaca aaaattggca
     2761 ctcggcactt aatggggggt cgtagtacgg aagcaaaatt cgcttccttt ccccccattt
     2821 ttttccaaat tccaaatttt tttcaaaaat tttccagcgc taccgctcgg caaaattgca
     2881 agcaattttt aaaatcaaac ccatgaggga atttcattcc ctcaaactcc cttgagcctc
     2941 ctccaaccga aatagaagga cgctgcgctt attatttcat tcagtcatcg gctttcataa
     3001 tctaacagac aacatcttcg ctgcaaagcc acgctacgct caagggcttt tacgctacga
     3061 taacgcctgt tttaacgatt atgccgataa ctaaacgaaa taaacgctaa aacgtctcag
     3121 aaacgatttt gagacgtttt aataaaaaat cgaccgaaaa ataaatataa atcggatata
     3181 caatcggcaa ttgacgaaac tgcaaaatat cctgtaaagg atacggattt tatgaccgat
     3241 gatgaagaaa agaatttgaa acgtttgtct gatttggagg aaggtttaca ccgtaaaagg
     3301 ttaatctcct atggtggttt gttaaaagaa atacataaaa aattaaacct tgatgacaca
     3361 gaagaaggcg atttgattca tacagatgat gacgaaaaag ccgatgaaga tggattttct
     3421 attattgcaa tgtggaattg ggaacggaaa aattatttta ttaaagagta gttcaacaaa
     3481 cgggccagtt tgttgaagat tagatgctat aattgttatt aaaaggattg aaggatgctt
     3541 aggaagacga gttattaata gctgaataag aacggtgctc tccaaatatt cttatttaga
     3601 aaagcaaatc taaaattatc tgaaaaggga atgagaatag tgaatggacc aataataatg
     3661 actagagaag aaagaatgaa gattgttcat gaaattaagg aacgaatatt ggataaatat
     3721 ggggatgatg ttaaggctat tggtgtttat ggctctcttg gtcgtcagac tgatgggccc
     3781 cgaaaagccc tgacaaccct tgttcctaaa aaggaataag cgttcggtca gtaaataata
     3841 gaaataaaaa atcagaccta agactgatga caaaaagaga aaattttgat aaaatagtct
     3901 tagaattaaa ttaaaaaggg aggccaaata taatgaaaaa tatgaatgac aatgatgttg
     3961 gatctcttta agaaggagga tatacaaatg gctagcagag gatcgcatca ccatcaccat
     4021 cacggggccg gagaccgcgg tcccgaattc gagctcggta cccggggatc cctcgaggtc
     4081 gacctgcagg gggaccatgg tctcagcgct tggagccacc cgcagttcga aaaataata
//
