LOCUS       sequence-177545-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-CBPNDHFR
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..126
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             151..171
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(726..744)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      740..787
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        852..943
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tccggaaata attttgttta actttaagaa ggagatatac atatgtctaa gcgacgatgg
       61 aaaaagaatt tcatagccgt ctcagcagcc aaccgcttta agaaaatctc atcctccggg
      121 gcacttctgg gatctggtgg tggtggtggt gaaaacctgt acttccaggg atccatcagt
      181 ctgattgcgg cgctagcggt agatcgcgtt atcggcatgg aaaacgccat gccatggaac
      241 ctgcctgccg atctcgcctg gtttaaacgc aacaccttaa ataaacccgt gattatgggg
      301 cgccatacct gggaatcaat cggtaggcct ttgcccggcc gcaaaaatat tatcctcagc
      361 agtcaacccg ggaccgatga tcgggttacc tgggttaaat cggtcgacga agccatcgcg
      421 gcgtgtggtg acgtaccaga aatcatggtg attggcggcg gacgcgttta tgaacagttc
      481 ttgccaaaag cgcaaaaact gtatctgacg catatcgatg cagaagtgga aggcgacacc
      541 cattttccgg attacgagcc ggatgactgg gaatcggtat tcagcgaatt ccacgatgct
      601 gatgcgcaga actcgcatag ctattgtttc gaaatcctcg agcgtcgtgc cggctaatgt
      661 acaacgcgtg cgcgctgatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      721 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
      781 ttttttgctg aaaggaggaa ctatatcggg attagcccgc ctaatgagcg ggcttttttt
      841 taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      901 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      961 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     1021 a
//
