LOCUS       sequence-177548-         405 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-FLAGNDHFR
FEATURES             Location/Qualifiers
     source          1..405
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..43
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gggatctggt ggtggtggtg gtgaaaacct gtacttccag ggatccatca gtctgattgc
       61 ggcgctagcg gtagatcgcg ttatcggcat ggaaaacgcc atgccatgga acctgcctgc
      121 cgatctcgcc tggtttaaac gcaacacctt aaataaaccc gtgattatgg ggcgccatac
      181 ctgggaatca atcggtaggc ctttgcccgg ccgcaaaaat attatcctca gcagtcaacc
      241 cgggaccgat gatcgggtta cctgggttaa atcggtcgac gaagccatcg cggcgtgtgg
      301 tgacgtacca gaaatcatgg tgattggcgg cggacgcgtt tatgaacagt tcttgccaaa
      361 agcgcaaaaa ctgtatctga cgcatatcga tgcagaagtg gaagg
//
