LOCUS       sequence-177549-         528 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-HPCNDHFR
FEATURES             Location/Qualifiers
     source          1..528
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(460..480)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gaaacaatag ctatgcgagt tctgcgcatc agcatcgtgg aattcgctga ataccgattc
       61 ccagtcatcc ggctcgtaat ccggaaaatg ggtgtcgcct tccacttctg catcgatatg
      121 cgtcagatac agtttttgcg cttttggcaa gaactgttca taaacgcgtc cgccgccaat
      181 caccatgatt tctggtacgt caccacacgc cgcgatggct tcgtcgaccg atttaaccca
      241 ggtaacccga tcatcggtcc cgggttgact gctgaggata atatttttgc ggccgggcaa
      301 aggcctaccg attgattccc aggtatggcg ccccataatc acgggtttat ttaaggtgtt
      361 gcgtttaaac caggcgagat cggcaggcag gttccatggc atggcgtttt ccatgccgat
      421 aacgcgatct accgctagcg ccgcaatcag actgatggat ccctggaagt acaggttttc
      481 accaccacca ccaccagatc ctttaccgtc gattaaacgt ggatctac
//
