LOCUS       sequence-177557-         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-HPCHISNDHFR
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(295..315)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(343..360)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(370..405)
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     RBS             419..441
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(478..497)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(479..497)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcgtccgccg ccaatcacca tgatttctgg tacgtcacca cacgccgcga tggcttcgtc
       61 gaccgattta acccaggtaa cccgatcatc ggtcccgggt tgactgctga ggataatatt
      121 tttgcggccg ggcaaaggcc taccgattga ttcccaggta tggcgcccca taatcacggg
      181 tttatttaag gtgttgcgtt taaaccaggc gagatcggca ggcaggttcc atggcatggc
      241 gttttccatg ccgataacgc gatctaccgc tagcgccgca atcagactga tggatccctg
      301 gaagtacagg ttttcaccac caccaccacc agatccgctg ctgtgatgat gatgatgatg
      361 gctagatcct ttaccgtcga ttaaacgtgg atctacctga tcttcagaca tatgtatatc
      421 tccttcttaa agttaaacaa aattatttcc ggagccggag ggaaaccgtt gtggtctccc
      481 tatagtgagt cgtattaatt tcgcgggatc gatgcatggt gccaacacct acatctgtat
      541 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg
      601 ccagttgttt accctcacaa cgttccagta accgggg
//
