LOCUS       sequence-17756-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Hausser A, Storz P, Martens S, Link G, Toker A, Pfizenmaier K
  TITLE     Protein kinase D regulates vesicular transport by phosphorylating 
            and activating phosphatidylinositol-4 kinase IIIbeta at the Golgi 
            complex.
  JOURNAL   Nat Cell Biol. 2005 Sep . 7(9):880-6.
  PUBMED    16100512
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2005 Sep . 7(9):880-6."
COMMENT     Sequence Label: pSUPER.PKD3.RNAi
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(112..128)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(113..129)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(157..175)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(196..212)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(196..212)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(209..231)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    220..236
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(244..250,251..268,269..274))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    289..310
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(427..444)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(678..697)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttggattt gggatcttat aagttctgta tgagaccaca gatccccgac gggactctct
       61 gcccgattca agagatcggg cagagagtcc cgtctttttg gaaaagctta tcgataccgt
      121 cgacctcgag ggggggcccg gtaccagctt ttgttccctt tagtgagggt taatttcgag
      181 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
      241 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
      301 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      361 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
      421 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      481 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      541 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      601 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      661 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      721 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      781 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      841 gctgggctgt gtgcacgaac cccccgttca gccgaacgc
//
