LOCUS       sequence-177563-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as 
            positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-DHFRFLAG
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..47)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             544..566
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(603..622)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(604..622)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cagcgcgcac gcgttgtaca ttacttgtca tcatcgtcct tgtagtcgcc ggcacgacgc
       61 tcgaggattt cgaaacaata gctatgcgag ttctgcgcat cagcatcgtg gaattcgctg
      121 aataccgatt cccagtcatc cggctcgtaa tccggaaaat gggtgtcgcc ttccacttct
      181 gcatcgatat gcgtcagata cagtttttgc gcttttggca agaactgttc ataaacgcgt
      241 ccgccgccaa tcaccatgat ttctggtacg tcaccacacg ccgcgatggc ttcgtcgacc
      301 gatttaaccc aggtaacccg atcatcggtc ccgggttgac tgctgaggat aatatttttg
      361 cggccgggca aaggcctacc gattgattcc caggtatggc gccccataat cacgggttta
      421 tttaaggtgt tgcgtttaaa ccaggcgaga tcggcaggca ggttccatgg catggcgttt
      481 tccatgccga taacgcgatc taccgctagc gccgcaatca gactgatgga tcccatatgt
      541 atatctcctt cttaaagtta aacaaaatta tttccggagc cggagggaaa ccgttgtggt
      601 ctccctatag tgagtcgtat taatttcgcg ggatcgatgc atggtgccaa cacctacatc
      661 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca
      721 taccgccagt tgtttaccct cacaacgt
//
