LOCUS       sequence-177564-        6219 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Streptococci shuttle plasmid for gene expression in 
            streptococci with P23 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Biswas I, Jha JK, Fromm N
  TITLE     Shuttle expression plasmids for genetic studies in Streptococcus 
            mutans.
  JOURNAL   Microbiology. 2008 Aug;154(Pt 8):2275-82. doi: 
            10.1099/mic.0.2008/019265-0.
  PUBMED    18667560
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology."; date: "2008-08"; volume: "154(Pt 8):2275-82. doi"; 
            pages: " 10.1099/mic.0.2008/019265-0"
COMMENT     Sequence Label: pIB190
FEATURES             Location/Qualifiers
     source          1..6219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3095..3112
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3200..3223
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(3314..3331)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3485..4073)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3565..3584)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aactaactca acgctagtag tggatttaat cccaaatgag ccaacagaac cagaaccaga
       61 aacagaatca gaacaagtaa cattggattt agaaatggaa gaagaaaaaa gcaatgactt
      121 cgtgtgaata atgcacgaaa tcgttgctta ttttttttaa aagcggtata ctagatataa
      181 cgaaacaacg aactgaatag aaacgaaaaa agagccatga cacatttata aaatgtttga
      241 cgacatttta taaatgcata gcccgataag attgccaaac caacgcttat cagttagtca
      301 gatgaactct tccctcgtaa gaagttattt aattaacttt gtttgaagac ggtatataac
      361 cgtactatca ttatataggg aaatcagaga gttttcaagt atctaagcta ctgaatttaa
      421 gaattgttaa gcaatcaatc ggaaatcgtt tgattgcttt ttttgtattc atttatagaa
      481 ggtggagttt gtatgaatca tgatgaatgt aaaacttata taaaaaatag tttattggag
      541 ataagaaaat tagcaaatat ctatacacta gaaacgttta agaaagagtt agaaaagaga
      601 aatatctact tagaaacaaa atcagataag tatttttctt cggaggggga agattatata
      661 tataagttaa tagaaaataa caaaataatt tattcgatta gtggaaaaaa attgacttat
      721 aaaggaaaaa aatctttttc aaaacatgca atattgaaac agttgaatga aaaagcaaac
      781 caagttaatt aaacaaccta ttttatagga tttataggaa aggagaacag ctgaatgaat
      841 atcccttttg ttgtagaaac tgtgcttcat gacggcttgt taaagtacaa atttaaaaat
      901 agtaaaattc gctcaatcac taccaagcca ggtaaaagca aaggggctat ttttgcgtat
      961 cgctcaaaat caagcatgat tggcggtcgt ggtgttgttc tgacttccga ggaagcgatt
     1021 caagaaaatc aagatacatt tacacattgg acacccaacg tttatcgtta tggaacgtat
     1081 gcagacgaaa accgttcata cacgaaagga cattctgaaa acaatttaag acaaatcaat
     1141 accttcttta ttgattttga tattcacacg gcaaaagaaa ctatttcagc aagcgatatt
     1201 ttaacaaccg ctattgattt aggttttatg cctactatga ttatcaaatc tgataaaggt
     1261 tatcaagcat attttgtttt agaaacgcca gtctatgtga cttcaaaatc agaatttaaa
     1321 tctgtcaaag cagccaaaat aatttcgcaa aatatccgag aatattttgg aaagtctttg
     1381 ccagttgatc taacgtgtaa tcattttggt attgctcgca taccaagaac ggacaatgta
     1441 gaattttttg atcctaatta ccgttattct ttcaaagaat ggcaagattg gtctttcaaa
     1501 caaacagata ataagggctt tactcgttca agtctaacgg ttttaagcgg tacagaaggc
     1561 aaaaaacaag tagatgaacc ctggtttaat ctcttattgc acgaaacgaa attttcagga
     1621 gaaaagggtt taatagggcg taataacgtc atgtttaccc tctctttagc ctactttagt
     1681 tcaggctatt caatcgaaac gtgcgaatat aatatgtttg agtttaataa tcgattagat
     1741 caacccttag aagaaaaaga agtaatcaaa attgttagaa gtgcctattc agaaaactat
     1801 caaggggcta atagggaata cattaccatt ctttgcaaag cttgggtatc aagtgattta
     1861 accagtaaag atttatttgt ccgtcaaggg tggtttaaat tcaagaaaaa aagaagcgaa
     1921 cgtcaacgtg ttcatttgtc agaatggaaa gaagatttaa tggcttatat tagcgaaaaa
     1981 agcgatgtat acaagcctta tttagtgacg accaaaaaag agattagaga agtgctaggc
     2041 attcctgaac ggacattaga taaattgctg aaggtactga aggcgaatca ggaaattttc
     2101 tttaagatta aaccaggaag aaatggtggc attcaacttg ctagtgttaa atcattgttg
     2161 ctatcgatca ttaaagtaaa aaaagaagaa aaagaaagct atataaaggc gctgacaaat
     2221 tcttttgact tagagcatac attcattcaa gagactttaa acaagctagc agaacgccct
     2281 aaaacggaca cacaactcga tttgtttagc tatgatacag gctgaaaata aaacccgcac
     2341 tatgccatta catttatatc tatgatacgt gtttgttttt tctttgctgt ttagcgaatg
     2401 attagcagaa atatacagag taagatttta attaattatt agggggagaa ggagagagta
     2461 gcccgaaaac ttttagttgg cttggactga acgaagtgag ggaaaggcta ctaaaacgtc
     2521 gaggggcagt gagagcgaag cgaacacttg attttttaat tttctatctt ttataggtca
     2581 ttagagtata cttatttgtc ctataaacta tttagcagca taatagattt attgaatagg
     2641 tcatttaagt tgagcatatt agaggaggaa aatcttggag aaatatttga agaacccgat
     2701 tacatggatt ggattagttc ttgtggttac gtggttttta actaaaagta gtgaattttt
     2761 gatttttggt gtgtgtgtct tgttgttagt atttgctagt caaagtgatt aaatagaatt
     2821 taaggctatt ggtgtttatg gctctcttgg tcgtcagact gatgggcccc gaaaagccct
     2881 gacaaccctt gttcctaaaa aggaataagc gttcggtcag taaataatag aaataaaaaa
     2941 tcagacctaa gactgatgac aaaaagagaa aattttgata aaatagtctt agaattaaat
     3001 taaaaaggga ggccaaatat aatgaaaaat atgaatgaca atgatgttgg atctctttaa
     3061 gaaggaggat atacaaatgg ctagcagagg atcgcatcac catcaccatc acggggccgg
     3121 agaccgcggt cccgaattcg agctcggtac ccggggatcc ctcgaggtcg acctgcaggg
     3181 ggaccatggt ctcagcgctt ggagccaccc gcagttcgaa aaataataag cttaagtgat
     3241 tagtcaaaga atggtgatga caattgtatc gacctgcagc caagcttatc gattctagac
     3301 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3361 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3421 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3481 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3541 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3601 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3661 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3721 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3781 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3841 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3901 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3961 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4021 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4081 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4141 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa atctagaatc
     4201 gatacgattt tgaagtggca acagataaaa aaaagcagtt taaaattgtt gctgaacttt
     4261 taaaacaagc aaatacaatc attgtcgcaa cagatagcga cagagaaggc gaaaacattg
     4321 cctggtcgat cattcataaa gcaaatgcct tttctaaaga taaaacgtat aaaagactat
     4381 ggatcaatag tttagaaaaa gatgtgatcc gtagcggttt tcaaaatttg caaccaggaa
     4441 tgaattacta tcccttttat caagaagcgc acaaaaagaa aaacgaaatg atacaccaat
     4501 cagtgcaaaa aaagatataa tgggagataa gacggttcgt gttcgtgctg acttgcacca
     4561 tatcataaaa atcgaaacag caaagaatgg cggaaacgta aaagaagtta tggaaataag
     4621 acttagaagc aaacttaaga gtgtgttgat agtgcagtat cttaaaattt tgtataatag
     4681 gaattgaagt taaattagat gctaaaaatt tgtaattaag aaggagtgat tacatgaaca
     4741 aaaatataaa atattctcaa aactttttaa cgagtgaaaa agtactcaac caaataataa
     4801 aacaattgaa tttaaaagaa accgataccg tttacgaaat tggaacaggt aaagggcatt
     4861 taacgacgaa actggctaaa ataagtaaac aggtaacgtc tattgaatta gacagtcatc
     4921 tattcaactt atcgtcagaa aaattaaaac tgaatactcg tgtcacttta attcaccaag
     4981 atattctaca gtttcaattc cctaacaaac agaggtataa aattgttggg agtattcctt
     5041 accatttaag cacacaaatt attaaaaaag tggtttttga aagccatgcg tctgacatct
     5101 atctgattgt tgaagaagga ttctacaagc gtaccttgga tattcaccga acactagggt
     5161 tgctcttgca cactcaagtc tcgattcagc aattgcttaa gctgccagcg gaatgctttc
     5221 atcctaaacc aaaagtaaac agtgtcttaa taaaacttac ccgccatacc acagatgttc
     5281 cagataaata ttggaagcta tatacgtact ttgtttcaaa atgggtcaat cgagaatatc
     5341 gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa acacgccaaa gtaaacaatt
     5401 taagtaccgt tacttatgag caagtattgt ctatttttaa tagttatcta ttatttaacg
     5461 ggaggaaata attctatgag tcgcttttgt aaatttggaa agttacacgt tactaaaggg
     5521 aatgtagata aattattagg tatactactg acagcttcca aggagctaaa gaggtcccta
     5581 gcgctcttat catggggaag ctcggatcat atgcaagaca aaataaactc gcaacagcac
     5641 ttggagaaat gggacgaatc gagaaaaccc tctttacgct ggattacata tctaataaag
     5701 ccgtaaggag acgggttcaa aaaggtttaa ataaaggaga agcaatcaat gcattagcta
     5761 gaattatatt ttttggacaa cgtggagaat ttagagaacg tgctctccaa gaccagttac
     5821 aaagagctag tgcactaaac ataattatta acgctataag tgtgtggaac actgtatata
     5881 tggaaaaagc cgtagaagaa ttaaaagcaa gaggagaatt tagagaagat ttaatgccat
     5941 atgcgtggcc gttaggatgg gaacatatca attttcttgg agaatacaaa tttgaaggat
     6001 tacatgacac tgggcaaatg aatttacgtc ctttacgtat aaaagagccg ttttattctt
     6061 aatataacgg ctctttttat agaaaaaatc cttagcgtgg tttttttccg aaatgctggc
     6121 ggtaccccaa gaattagaaa tgagtagatc aaattattca cgaatagaat caggaaaatc
     6181 agatccaacc ataaaaacac tagaacaaat tgcaaagtt
//
