LOCUS       sequence-177565-        6089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Streptococci shuttle plasmid for gene expression in 
            streptococci with Pveg promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Biswas I, Jha JK, Fromm N
  TITLE     Shuttle expression plasmids for genetic studies in Streptococcus 
            mutans.
  JOURNAL   Microbiology. 2008 Aug;154(Pt 8):2275-82. doi: 
            10.1099/mic.0.2008/019265-0.
  PUBMED    18667560
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology."; date: "2008-08"; volume: "154(Pt 8):2275-82. doi"; 
            pages: " 10.1099/mic.0.2008/019265-0"
COMMENT     Sequence Label: pIB185
FEATURES             Location/Qualifiers
     source          1..6089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1432..1449)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1603..2191)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1683..1702)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaaaaagaag taatcaaaat tgttagaagt gcctattcag aaaactatca aggggctaat
       61 agggaataca ttaccattct ttgcaaagct tgggtatcaa gtgatttaac cagtaaagat
      121 ttatttgtcc gtcaagggtg gtttaaattc aagaaaaaaa gaagcgaacg tcaacgtgtt
      181 catttgtcag aatggaaaga agatttaatg gcttatatta gcgaaaaaag cgatgtatac
      241 aagccttatt tagtgacgac caaaaaagag attagagaag tgctaggcat tcctgaacgg
      301 acattagata aattgctgaa ggtactgaag gcgaatcagg aaattttctt taagattaaa
      361 ccaggaagaa atggtggcat tcaacttgct agtgttaaat cattgttgct atcgatcatt
      421 aaagtaaaaa aagaagaaaa agaaagctat ataaaggcgc tgacaaattc ttttgactta
      481 gagcatacat tcattcaaga gactttaaac aagctagcag aacgccctaa aacggacaca
      541 caactcgatt tgtttagcta tgatacaggc tgaaaataaa acccgcacta tgccattaca
      601 tttatatcta tgatacgtgt ttgttttttc tttgctgttt agcgaatgat tagcagaaat
      661 atacagagta agattttaat taattattag ggggagaagg agagagtagc ccgaaaactt
      721 ttagttggct tggactgaac gaagtgaggg aaaggctact aaaacgtcga ggggcagtga
      781 gagcgaagcg aacacttgat tttttaattt tctatctttt ataggtcatt agagtatact
      841 tatttgtcct ataaactatt tagcagcata atagatttat tgaataggtc atttaagttg
      901 agcatattag aggaggaaaa tcttggagaa atatttgaag aacccgatta catggattgg
      961 attagttctt gtggttacgt ggtttttaac taaaagtagt gaatttttga tttttggtgt
     1021 gtgtgtcttg ttgttagtat ttgctagtca aagtgattaa atagaattta aggctattgg
     1081 tgtttatggc tctcttggtc gtcagactga tgggcccctg cagaggagag ctatgcgggt
     1141 tttttatttt acataatgat acataattta ccgaaacttg cggaacataa ttgaggaatc
     1201 atagaatttt gtcaaaataa ttttattgac aacgtcttat taacgttgat ataatttaaa
     1261 ttttatttga caaaaatggg ctcgtgttgt acaataaatg gatccccgcg gtacccggga
     1321 attctagagc tcgagatcta tcgataagct taagtgatta gtcaaagaat ggtgatgaca
     1381 attgtatcga cctgcagcca agcttatcga ttctagacgc tcttccgctt cctcgctcac
     1441 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1501 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1561 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1621 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1681 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1741 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1801 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1861 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1921 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1981 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2041 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2101 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2161 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2221 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2281 gatcttcacc tagatccttt taaattaaat ctagaatcga tacgattttg aagtggcaac
     2341 agataaaaaa aagcagttta aaattgttgc tgaactttta aaacaagcaa atacaatcat
     2401 tgtcgcaaca gatagcgaca gagaaggcga aaacattgcc tggtcgatca ttcataaagc
     2461 aaatgccttt tctaaagata aaacgtataa aagactatgg atcaatagtt tagaaaaaga
     2521 tgtgatccgt agcggttttc aaaatttgca accaggaatg aattactatc ccttttatca
     2581 agaagcgcac aaaaagaaaa acgaaatgat acaccaatca gtgcaaaaaa agatataatg
     2641 ggagataaga cggttcgtgt tcgtgctgac ttgcaccata tcataaaaat cgaaacagca
     2701 aagaatggcg gaaacgtaaa agaagttatg gaaataagac ttagaagcaa acttaagagt
     2761 gtgttgatag tgcagtatct taaaattttg tataatagga attgaagtta aattagatgc
     2821 taaaaatttg taattaagaa ggagtgatta catgaacaaa aatataaaat attctcaaaa
     2881 ctttttaacg agtgaaaaag tactcaacca aataataaaa caattgaatt taaaagaaac
     2941 cgataccgtt tacgaaattg gaacaggtaa agggcattta acgacgaaac tggctaaaat
     3001 aagtaaacag gtaacgtcta ttgaattaga cagtcatcta ttcaacttat cgtcagaaaa
     3061 attaaaactg aatactcgtg tcactttaat tcaccaagat attctacagt ttcaattccc
     3121 taacaaacag aggtataaaa ttgttgggag tattccttac catttaagca cacaaattat
     3181 taaaaaagtg gtttttgaaa gccatgcgtc tgacatctat ctgattgttg aagaaggatt
     3241 ctacaagcgt accttggata ttcaccgaac actagggttg ctcttgcaca ctcaagtctc
     3301 gattcagcaa ttgcttaagc tgccagcgga atgctttcat cctaaaccaa aagtaaacag
     3361 tgtcttaata aaacttaccc gccataccac agatgttcca gataaatatt ggaagctata
     3421 tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt caactgttta ctaaaaatca
     3481 gtttcatcaa gcaatgaaac acgccaaagt aaacaattta agtaccgtta cttatgagca
     3541 agtattgtct atttttaata gttatctatt atttaacggg aggaaataat tctatgagtc
     3601 gcttttgtaa atttggaaag ttacacgtta ctaaagggaa tgtagataaa ttattaggta
     3661 tactactgac agcttccaag gagctaaaga ggtccctagc gctcttatca tggggaagct
     3721 cggatcatat gcaagacaaa ataaactcgc aacagcactt ggagaaatgg gacgaatcga
     3781 gaaaaccctc tttacgctgg attacatatc taataaagcc gtaaggagac gggttcaaaa
     3841 aggtttaaat aaaggagaag caatcaatgc attagctaga attatatttt ttggacaacg
     3901 tggagaattt agagaacgtg ctctccaaga ccagttacaa agagctagtg cactaaacat
     3961 aattattaac gctataagtg tgtggaacac tgtatatatg gaaaaagccg tagaagaatt
     4021 aaaagcaaga ggagaattta gagaagattt aatgccatat gcgtggccgt taggatggga
     4081 acatatcaat tttcttggag aatacaaatt tgaaggatta catgacactg ggcaaatgaa
     4141 tttacgtcct ttacgtataa aagagccgtt ttattcttaa tataacggct ctttttatag
     4201 aaaaaatcct tagcgtggtt tttttccgaa atgctggcgg taccccaaga attagaaatg
     4261 agtagatcaa attattcacg aatagaatca ggaaaatcag atccaaccat aaaaacacta
     4321 gaacaaattg caaagttaac taactcaacg ctagtagtgg atttaatccc aaatgagcca
     4381 acagaaccag aaccagaaac agaatcagaa caagtaacat tggatttaga aatggaagaa
     4441 gaaaaaagca atgacttcgt gtgaataatg cacgaaatcg ttgcttattt tttttaaaag
     4501 cggtatacta gatataacga aacaacgaac tgaatagaaa cgaaaaaaga gccatgacac
     4561 atttataaaa tgtttgacga cattttataa atgcatagcc cgataagatt gccaaaccaa
     4621 cgcttatcag ttagtcagat gaactcttcc ctcgtaagaa gttatttaat taactttgtt
     4681 tgaagacggt atataaccgt actatcatta tatagggaaa tcagagagtt ttcaagtatc
     4741 taagctactg aatttaagaa ttgttaagca atcaatcgga aatcgtttga ttgctttttt
     4801 tgtattcatt tatagaaggt ggagtttgta tgaatcatga tgaatgtaaa acttatataa
     4861 aaaatagttt attggagata agaaaattag caaatatcta tacactagaa acgtttaaga
     4921 aagagttaga aaagagaaat atctacttag aaacaaaatc agataagtat ttttcttcgg
     4981 agggggaaga ttatatatat aagttaatag aaaataacaa aataatttat tcgattagtg
     5041 gaaaaaaatt gacttataaa ggaaaaaaat ctttttcaaa acatgcaata ttgaaacagt
     5101 tgaatgaaaa agcaaaccaa gttaattaaa caacctattt tataggattt ataggaaagg
     5161 agaacagctg aatgaatatc ccttttgttg tagaaactgt gcttcatgac ggcttgttaa
     5221 agtacaaatt taaaaatagt aaaattcgct caatcactac caagccaggt aaaagcaaag
     5281 gggctatttt tgcgtatcgc tcaaaatcaa gcatgattgg cggtcgtggt gttgttctga
     5341 cttccgagga agcgattcaa gaaaatcaag atacatttac acattggaca cccaacgttt
     5401 atcgttatgg aacgtatgca gacgaaaacc gttcatacac gaaaggacat tctgaaaaca
     5461 atttaagaca aatcaatacc ttctttattg attttgatat tcacacggca aaagaaacta
     5521 tttcagcaag cgatatttta acaaccgcta ttgatttagg ttttatgcct actatgatta
     5581 tcaaatctga taaaggttat caagcatatt ttgttttaga aacgccagtc tatgtgactt
     5641 caaaatcaga atttaaatct gtcaaagcag ccaaaataat ttcgcaaaat atccgagaat
     5701 attttggaaa gtctttgcca gttgatctaa cgtgtaatca ttttggtatt gctcgcatac
     5761 caagaacgga caatgtagaa ttttttgatc ctaattaccg ttattctttc aaagaatggc
     5821 aagattggtc tttcaaacaa acagataata agggctttac tcgttcaagt ctaacggttt
     5881 taagcggtac agaaggcaaa aaacaagtag atgaaccctg gtttaatctc ttattgcacg
     5941 aaacgaaatt ttcaggagaa aagggtttaa tagggcgtaa taacgtcatg tttaccctct
     6001 ctttagccta ctttagttca ggctattcaa tcgaaacgtg cgaatataat atgtttgagt
     6061 ttaataatcg attagatcaa cccttagaa
//
