LOCUS       sequence-177574-        8553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cytosolic APEX2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Hung V, Lam SS, Udeshi ND, Svinkina T, Guzman G, Mootha VK, Carr SA,
            Ting AY
  TITLE     Proteomic mapping of cytosol-facing outer mitochondrial and ER 
            membranes in living human cells by proximity biotinylation.
  JOURNAL   Elife. 2017 Apr 25;6. pii: e24463. doi: 10.7554/eLife.24463.
  PUBMED    28441135
REFERENCE   2  (bases 1 to 8553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-25"; volume: "6. pii: e24463. doi"; pages: " 
            10.7554/eLife.24463"
COMMENT     Sequence Label: pLX304-Flag-APEX2-NES
FEATURES             Location/Qualifiers
     source          1..8553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             640..663
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             664..1410
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             1417..1443
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     protein_bind    complement(1451..1475)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1477..1518
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1560..2148
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1613..1633)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2031..2042)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2220..2453
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2531..2652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2568..2587)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2622..2641
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2692..2827
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2847..2866)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2848..2866)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2876..2893)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2876..2892)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2885..2907)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3034..3489
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3121..3140)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3331..3352
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3515..3619
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3620..3688,3689..4480)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3838..3857)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4651..5239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5140..5159
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5393..5410
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5527..5548
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5563..5568,5569..5586,5587..5593)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5601..5617
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5606..5628
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5625..5641
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5625..5641
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5660..5680
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5662..5680
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5708..5934
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5935..6115
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6162..6287
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6780..7013
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7198..7242
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7417..7917
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7836..7854
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7929..8324
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8384..8501
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagcaggctt cgaaggagat agaaccatgg actacaagga tgacgacgat
      661 aagggaaagt cttacccaac tgtgagtgct gattaccagg acgccgttga gaaggcgaag
      721 aagaagctca gaggcttcat cgctgagaag agatgcgctc ctctaatgct ccgtttggca
      781 ttccactctg ctggaacctt tgacaagggc acgaagaccg gtggaccctt cggaaccatc
      841 aagcaccctg ccgaactggc tcacagcgct aacaacggtc ttgacatcgc tgttaggctt
      901 ttggagccac tcaaggcgga gttccctatt ttgagctacg ccgatttcta ccagttggct
      961 ggcgttgttg ccgttgaggt cacgggtgga cctaaggttc cattccaccc tggaagagag
     1021 gacaagcctg agccaccacc agagggtcgc ttgcccgatc ccactaaggg ttctgaccat
     1081 ttgagagatg tgtttggcaa agctatgggg cttactgacc aagatatcgt tgctctatct
     1141 gggggtcaca ctattggagc tgcacacaag gagcgttctg gatttgaggg tccctggacc
     1201 tctaatcctc ttattttcga caactcatac ttcacggagt tgttgagtgg tgagaaggaa
     1261 ggtctccttc agctaccttc tgacaaggct cttttgtctg accctgtatt ccgccctctc
     1321 gttgacaaat atgcagcgga cgaagatgcc ttctttgctg attacgctga ggctcaccaa
     1381 aagctttccg agcttgggtt tgctgatgcc ctgcagctgc ctcccctgga gcgcctgacc
     1441 ctggactaag acccagcttt cttgtacaaa gtggttggta agcctatccc taaccctctc
     1501 ctcggtctcg attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca
     1561 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     1621 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     1681 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     1741 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     1801 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     1861 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     1921 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     1981 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     2041 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     2101 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag
     2161 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     2221 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     2281 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     2341 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     2401 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta
     2461 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     2521 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     2581 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     2641 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     2701 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     2761 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     2821 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     2881 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     2941 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     3001 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     3061 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     3121 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3181 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     3241 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     3301 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3361 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     3421 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     3481 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3541 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3601 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3661 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3721 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     3781 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     3841 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     3901 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     3961 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4021 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4081 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4141 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4201 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4261 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4321 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4381 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4441 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4501 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     4561 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4621 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4681 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4741 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4801 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4861 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4921 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4981 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5041 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5101 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5161 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5221 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5281 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     5341 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     5401 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     5461 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     5521 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     5581 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     5641 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     5701 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
     5761 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
     5821 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
     5881 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
     5941 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6001 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6061 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     6121 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     6181 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     6241 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     6301 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     6361 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     6421 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     6481 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     6541 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     6601 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     6661 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     6721 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     6781 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     6841 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     6901 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     6961 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     7021 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     7081 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     7141 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     7201 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     7261 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     7321 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     7381 tcgtttcaga cccacctccc aaccccgagg ggacccttgc gccttttcca aggcagccct
     7441 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac
     7501 cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc
     7561 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg
     7621 ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac
     7681 ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc
     7741 tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt
     7801 ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg
     7861 gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg
     7921 gtaccaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     7981 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
     8041 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     8101 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     8161 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
     8221 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
     8281 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggcctgcag ctgcagtagt
     8341 aagaattcta gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg
     8401 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     8461 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     8521 gcagagatcc actttggcgc cggctcgagg ggg
//
