LOCUS       sequence-177586-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of 
            the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 
            2009 ), expressed via the TEF1 promoter. HO locus integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Papagiannakis A, Niebel B, Wit EC, Heinemann M
  TITLE     Autonomous Metabolic Oscillations Robustly Gate the Early and Late 
            Cell Cycle.
  JOURNAL   Mol Cell. 2017 Jan 19;65(2):285-295. doi: 
            10.1016/j.molcel.2016.11.018. Epub 2016 Dec 15.
  PUBMED    27989441
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.11.018"; journalName: "Mol Cell"; date: 
            "2017-01-19- 19"; volume: "65"; issue: "2"; pages: "285-295"
COMMENT     Sequence Label: pTEF:ATP
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        109..507
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 aggagggtat tctgggcctc catgtcgctg gccgggtgac ccggcgggga cgaggcaagc
       61 taaacagatc tggcgcgcct taattaaccc gcagctggaa ttccacacca tagcttcaaa
      121 atgtttctac tcctttttta ctcttccaga ttttctcgga ctccgcgcat cgccgtacca
      181 cttcaaaaca cccaagcaca gcatactaaa tttcccctct ttcttcctct agggtgtcgt
      241 taattacccg tactaaaggt ttggaaaaga aaaaagagac cgcctcgttt ctttttcttc
      301 gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt cttgaaaatt ttttttttga
      361 tttttttctc tttcgatgac ctcccattga tatttaagtt aataaacggt cttcaatttc
      421 tcaagtttca gtttcatttt tcttgttcta ttacaacttt ttttacttct tgctcattag
      481 aaagaaagca tagcaatcta atctaagttt cctaggttat taaaatggtt tctaagggtg
      541 aagaattatt cactggtgtt gttccaatct tggttgaatt ggatggtgat gttaacggtc
      601 acagattttc tgtttctggt gaaggtgaag gtgatgctac ttatggtaaa ttgaccttga
      661 agttcatctg taccacaggt aaattgccag ttccatggcc aactttggtt actactttga
      721 cttggggtgt tcaatgtttt gctagatacc cagatcatat gaagcaacac gatttcttca
      781 aatccgctat gccagaaggt tacgttcaag aaagaaccat cttcttcaag gatgacggta
      841 actacaaaac tagagccgaa gttaagttcg aaggtgatac cttggttaac agaatcgaat
      901 tgaagggtat cgacttcaaa gaagatggta acatcttggg tcacaagttg gaatacaact
      961 acatctccca taacgtttac attaccgccg ataagcaaaa ga
//
