LOCUS       sequence-177589-        6086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Streptococci shuttle plasmid for gene expression in 
            streptococci with P23 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Biswas I, Jha JK, Fromm N
  TITLE     Shuttle expression plasmids for genetic studies in Streptococcus 
            mutans.
  JOURNAL   Microbiology. 2008 Aug;154(Pt 8):2275-82. doi: 
            10.1099/mic.0.2008/019265-0.
  PUBMED    18667560
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology."; date: "2008-08"; volume: "154(Pt 8):2275-82. doi"; 
            pages: " 10.1099/mic.0.2008/019265-0"
COMMENT     Sequence Label: pIB184 (EM)
FEATURES             Location/Qualifiers
     source          1..6086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      5233..5821
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5722..5741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5975..5992
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaaaagccc tgacaaccct tgttcctaaa aaggaataag cgttcggtca gtaaataata
       61 gaaataaaaa atcagaccta agactgatga caaaaagaga aaattttgat aaaatagtct
      121 tagaattaaa ttaaaaaggg aggccaaata taatgaaaaa tatgaatgac aatgatgttg
      181 gatccccgcg gtacccggga attctagagc tcgagatcta tcgataagct taagtgatta
      241 gtcaaagaat ggtgatgaca attgtaaatt ctatttaatc actttgacta gcaaatacta
      301 acaacaagac acacacacca aaaatcaaaa attcactact tttagttaaa aaccacgtaa
      361 ccacaagaac taatccaatc catgtaatcg ggttcttcaa atatttctcc aagattttcc
      421 tcctctaata tgctcaactt aaatgaccta ttcaataaat ctattatgct gctaaatagt
      481 ttataggaca aataagtata ctctaatgac ctataaaaga tagaaaatta aaaaatcaag
      541 tgttcgcttc gctctcactg cccctcgacg ttttagtagc ctttccctca cttcgttcag
      601 tccaagccaa ctaaaagttt tcgggctact ctctccttct ccccctaata attaattaaa
      661 atcttactct gtatatttct gctaatcatt cgctaaacag caaagaaaaa acaaacacgt
      721 atcatagata taaatgtaat ggcatagtgc gggttttatt ttcagcctgt atcatagcta
      781 aacaaatcga gttgtgtgtc cgttttaggg cgttctgcta gcttgtttaa agtctcttga
      841 atgaatgtat gctctaagtc aaaagaattt gtcagcgcct ttatatagct ttctttttct
      901 tcttttttta ctttaatgat cgatagcaac aatgatttaa cactagcaag ttgaatgcca
      961 ccatttcttc ctggtttaat cttaaagaaa atttcctgat tcgccttcag taccttcagc
     1021 aatttatcta atgtccgttc aggaatgcct agcacttctc taatctcttt tttggtcgtc
     1081 actaaataag gcttgtatac atcgcttttt tcgctaatat aagccattaa atcttctttc
     1141 cattctgaca aatgaacacg ttgacgttcg cttctttttt tcttgaattt aaaccaccct
     1201 tgacggacaa ataaatcttt actggttaaa tcacttgata cccaagcttt gcaaagaatg
     1261 gtaatgtatt ccctattagc cccttgatag ttttctgaat aggcacttct aacaattttg
     1321 attacttctt tttcttctaa gggttgatct aatcgattat taaactcaaa catattatat
     1381 tcgcacgttt cgattgaata gcctgaacta aagtaggcta aagagagggt aaacatgacg
     1441 ttattacgcc ctattaaacc cttttctcct gaaaatttcg tttcgtgcaa taagagatta
     1501 aaccagggtt catctacttg ttttttgcct tctgtaccgc ttaaaaccgt tagacttgaa
     1561 cgagtaaagc ccttattatc tgtttgtttg aaagaccaat cttgccattc tttgaaagaa
     1621 taacggtaat taggatcaaa aaattctaca ttgtccgttc ttggtatgcg agcaatacca
     1681 aaatgattac acgttagatc aactggcaaa gactttccaa aatattctcg gatattttgc
     1741 gaaattattt tggctgcttt gacagattta aattctgatt ttgaagtcac atagactggc
     1801 gtttctaaaa caaaatatgc ttgataacct ttatcagatt tgataatcat agtaggcata
     1861 aaacctaaat caatagcggt tgttaaaata tcgcttgctg aaatagtttc ttttgccgtg
     1921 tgaatatcaa aatcaataaa gaaggtattg atttgtctta aattgttttc agaatgtcct
     1981 ttcgtgtatg aacggttttc gtctgcatac gttccataac gataaacgtt gggtgtccaa
     2041 tgtgtaaatg tatcttgatt ttcttgaatc gcttcctcgg aagtcagaac aacaccacga
     2101 ccgccaatca tgcttgattt tgagcgatac gcaaaaatag cccctttgct tttacctggc
     2161 ttggtagtga ttgagcgaat tttactattt ttaaatttgt actttaacaa gccgtcatga
     2221 agcacagttt ctacaacaaa agggatattc attcagctgt tctcctttcc tataaatcct
     2281 ataaaatagg ttgtttaatt aacttggttt gctttttcat tcaactgttt caatattgca
     2341 tgttttgaaa aagatttttt tcctttataa gtcaattttt ttccactaat cgaataaatt
     2401 attttgttat tttctattaa cttatatata taatcttccc cctccgaaga aaaatactta
     2461 tctgattttg tttctaagta gatatttctc ttttctaact ctttcttaaa cgtttctagt
     2521 gtatagatat ttgctaattt tcttatctcc aataaactat tttttatata agttttacat
     2581 tcatcatgat tcatacaaac tccaccttct ataaatgaat acaaaaaaag caatcaaacg
     2641 atttccgatt gattgcttaa caattcttaa attcagtagc ttagatactt gaaaactctc
     2701 tgatttccct atataatgat agtacggtta tataccgtct tcaaacaaag ttaattaaat
     2761 aacttcttac gagggaagag ttcatctgac taactgataa gcgttggttt ggcaatctta
     2821 tcgggctatg catttataaa atgtcgtcaa acattttata aatgtgtcat ggctcttttt
     2881 tcgtttctat tcagttcgtt gtttcgttat atctagtata ccgcttttaa aaaaaataag
     2941 caacgatttc gtgcattatt cacacgaagt cattgctttt ttcttcttcc atttctaaat
     3001 ccaatgttac ttgttctgat tctgtttctg gttctggttc tgttggctca tttgggatta
     3061 aatccactac tagcgttgag ttagttaact ttgcaatttg ttctagtgtt tttatggttg
     3121 gatctgattt tcctgattct attcgtgaat aatttgatct actcatttct aattcttggg
     3181 gtaccgccag catttcggaa aaaaaccacg ctaaggattt tttctataaa aagagccgtt
     3241 atattaagaa taaaacggct cttttatacg taaaggacgt aaattcattt gcccagtgtc
     3301 atgtaatcct tcaaatttgt attctccaag aaaattgata tgttcccatc ctaacggcca
     3361 cgcatatggc attaaatctt ctctaaattc tcctcttgct tttaattctt ctacggcttt
     3421 ttccatatat acagtgttcc acacacttat agcgttaata attatgttta gtgcactagc
     3481 tctttgtaac tggtcttgga gagcacgttc tctaaattct ccacgttgtc caaaaaatat
     3541 aattctagct aatgcattga ttgcttctcc tttatttaaa cctttttgaa cccgtctcct
     3601 tacggcttta ttagatatgt aatccagcgt aaagagggtt ttctcgattc gtcccatttc
     3661 tccaagtgct gttgcgagtt tattttgtct tgcatatgat ccgagcttcc ccatgataag
     3721 agcgctaggg acctctttag ctccttggaa gctgtcagta gtatacctaa taatttatct
     3781 acattccctt tagtaacgtg taactttcca aatttacaaa agcgactcat agaattattt
     3841 cctcccgtta aataatagat aactattaaa aatagacaat acttgctcat aagtaacggt
     3901 acttaaattg tttactttgg cgtgtttcat tgcttgatga aactgatttt tagtaaacag
     3961 ttgacgatat tctcgattga cccattttga aacaaagtac gtatatagct tccaatattt
     4021 atctggaaca tctgtggtat ggcgggtaag ttttattaag acactgttta cttttggttt
     4081 aggatgaaag cattccgctg gcagcttaag caattgctga atcgagactt gagtgtgcaa
     4141 gagcaaccct agtgttcggt gaatatccaa ggtacgcttg tagaatcctt cttcaacaat
     4201 cagatagatg tcagacgcat ggctttcaaa aaccactttt ttaataattt gtgtgcttaa
     4261 atggtaagga atactcccaa caattttata cctctgtttg ttagggaatt gaaactgtag
     4321 aatatcttgg tgaattaaag tgacacgagt attcagtttt aatttttctg acgataagtt
     4381 gaatagatga ctgtctaatt caatagacgt tacctgttta cttattttag ccagtttcgt
     4441 cgttaaatgc cctttacctg ttccaatttc gtaaacggta tcggtttctt ttaaattcaa
     4501 ttgttttatt atttggttga gtactttttc actcgttaaa aagttttgag aatattttat
     4561 atttttgttc atgtaatcac tccttcttaa ttacaaattt ttagcatcta atttaacttc
     4621 aattcctatt atacaaaatt ttaagatact gcactatcaa cacactctta agtttgcttc
     4681 taagtcttat ttccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta
     4741 tgatatggtg caagtcagca cgaacacgaa ccgtcttatc tcccattata tctttttttg
     4801 cactgattgg tgtatcattt cgtttttctt tttgtgcgct tcttgataaa agggatagta
     4861 attcattcct ggttgcaaat tttgaaaacc gctacggatc acatcttttt ctaaactatt
     4921 gatccatagt cttttatacg ttttatcttt agaaaaggca tttgctttat gaatgatcga
     4981 ccaggcaatg ttttcgcctt ctctgtcgct atctgttgcg acaatgattg tatttgcttg
     5041 ttttaaaagt tcagcaacaa ttttaaactg ctttttttta tctgttgcca cttcaaaatc
     5101 gtatcgattc tagatttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5161 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5221 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5281 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5341 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5401 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5461 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5521 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5581 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5641 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5701 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5761 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5821 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5881 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     5941 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6001 agagcgtcta gaatcgataa gcttggctgc aggtcgataa ggctattggt gtttatggct
     6061 ctcttggtcg tcagactgat gggccc
//
