LOCUS       sequence-177604-        7394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter for mouse MMP10 2.5kb promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Wu L, Chien WM, Hartman ME, Moussavi-Harami F, Liu Y, Chin MT
  TITLE     Regulation of MMP10 expression by the transcription factor CHF1/Hey2
            is mediated by multiple E boxes.
  JOURNAL   Biochem Biophys Res Commun. 2011 Dec 2;415(4):662-8. doi: 
            10.1016/j.bbrc.2011.10.132. Epub 2011 Nov 3.
  PUBMED    22079635
REFERENCE   2  (bases 1 to 7394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2011.10.132"; journalName: "Biochem Biophys Res 
            Commun"; date: "2011-12-2- 2"; volume: "415"; issue: "4"; pages: 
            "662-8"
COMMENT     Sequence Label: -2.5Kb MMP10
FEATURES             Location/Qualifiers
     source          1..7394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(112..129)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(283..871)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(363..382)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1042..1833,1834..1902))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1665..1684
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1903..2007)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2034..2489
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2121..2140)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2331..2352
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    2620..2668
                     /note="synthetic polyadenylation signal"
     misc_feature    2682..2773
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2722..2741
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      5438..5447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5444..7096
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5510..5528)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    7137..7258
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7148..7167)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7202..7221
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
       61 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac
      121 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      181 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      241 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      301 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      361 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      421 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      481 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      541 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      601 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      661 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      721 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      781 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      841 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      901 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
      961 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1021 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1081 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     1141 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     1201 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1261 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1321 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1381 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1441 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1501 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1561 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1621 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1681 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1741 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1801 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1861 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1921 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1981 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc
     2041 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     2101 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     2161 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     2221 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     2281 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2341 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     2401 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2461 ttttaacaaa atattaacgc ttacaatttg ccattcgcca ttcaggctgc gcaactgttg
     2521 ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac catgataagt
     2581 aagtaatatt aaggtacggg aggtacttgg agcggccgca ataaaatatc tttattttca
     2641 ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa
     2701 aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc
     2761 agaacatttc tctatcgata ggtaccgagc tcttacgcgt gctagtaacg gccgccagtg
     2821 tgctggaatt cgcccttaat tcaaactcga gccttcatta gaatctttcc ttgtacccct
     2881 cccataccca tttcttgaag aatcaatcat tttagaatag atattgttta gatctggaaa
     2941 tcccctactc cccccattct cctttcccct ctgagggcct ataaaaactg ggacctcttt
     3001 cccctcgagg tcaactcctc tacccctgcg tgggatatga gtcatcccca gagctctggc
     3061 tttccctgaa gcctcatgtg gtttgcaaca atcttggtct atcgtgagtt cttgggtgtc
     3121 tgttattgtc ctgaggccta agcgaggggc tccttttgga gtctttcaat accatcacat
     3181 caatggttgg caaggcaact cagtagaagg aaaagggtcc cacaagtatg caaaagagtc
     3241 agggacactg ctaccccttc ctattgttag gattcccaca agaacaccaa gttagcttca
     3301 gccataacat atattcaaag gacccaggtc agacccccac aggctccctg gtttctacat
     3361 gccaccctga gtcccagtca gttgattctg tgggctgtat tcttctggtg tgtccctgac
     3421 ccctctgctt tctcatatcc ttcctacccc tcctcagcag gactcccaca gctccaccgg
     3481 tgtgtctgtg gaactctgca tctgctccca tcagttgctg gatgaagtct ctctggtgct
     3541 aggctctgga tccgaacact ctgcaggcag aacaaactgg aagccggagt ttttgtgatt
     3601 gtgttggtgc cccagtccct ccttgaggcc ttgcctggtt acagaaggtg gctggttcag
     3661 gatccatttc tctatcacta gcagtcttac aaagggatac tcttggagtt tacactgcac
     3721 tagctttctt cttcaccccc aaaacgcctc acaaatacag cttttctccc agtatttctc
     3781 ccttccccac cacctgatcc tttgttccca ctcctacctg caaccagtcc atcggctaaa
     3841 tctattctct ctccccttcc cagggaaatt tttgtgtccc ttcttaacct accaacccag
     3901 agaagccata acatttttga tcggcatata agtatggtga gctagtcttc tgtgaaatca
     3961 ttcttcagtt tttatgattc taatcttgac cttcttaggt tgttctgcag attaaagata
     4021 acatgtagtt aaaaatttca cataaaagag ctctgttcta ggattccact ttgccttaat
     4081 gaactgcata agcctgcttg ttttactcta taaatattcg gtacatttaa taaataataa
     4141 cttacttaaa aataaacagg actccattac ccccaagtta ttaatctcct tcaggtgtgg
     4201 tggcctaaag tagagttctt ttatccacca aaatcactcc tgcaaggcac accctttcat
     4261 ggttagtcaa ccacagtcaa aatcataact ctgatcaaca agaaccatca gcaggatgtg
     4321 ccaccaacaa caaaggaatg attcaaatga atatgaggca tctcagaatg agtcgactca
     4381 gcacctcagg cagttagcaa tctgtcctgc taacaagccc tatcagctaa taacccagga
     4441 ataaagtaaa aaggcacttg ctgcccgcat gaagtacaca taaaaccaag accttgtttg
     4501 ttttccatag ggaaagcaat cacgggataa aattacatac ttgctcaagt ctagctgatg
     4561 gttcagtctg tcagcctctt tgcctctctg aattttaggt tggcatattc tctgtgtagt
     4621 gcctacacta agccacaagc tgacttggct ggcctagtgt acacatccct catccaagct
     4681 gtggctcaaa ggcctcaaag cctcctggtt taggaagagg aggacagagg taggaagaat
     4741 attttcattt aatagttaat taactgattt ccatttgaca cgtctttatg gagccagtat
     4801 atgcttatag caattattct gaaacagaga gtaggtgggc cccaagcttc ttacctctgt
     4861 acatttcctc ctccgtattg actgctttct cagagtactc ttaccccagg gctggaaata
     4921 cctcttaccc aaggactcca gcaatggagt ttctgccatt atagcttcct agacagcagt
     4981 ccttacattc actttgcaat ctctttccat taaactattc actccttgcc tgaatgtatg
     5041 taagcctaag gcatgttttt aaaaattaca ctgctttcaa aatatctaaa cattttgcaa
     5101 atattaatta cccagacagt cccttttcct ctttgtaaac acagaagcca catccttgag
     5161 attaagcatt ctttcaagtt tactaggttg agggattcac tgatgtaagc tattctctct
     5221 tcatcttatg aatgttcttg tagtcatttg gattaaaaat agcacccatg tgagtcatgc
     5281 ttagtgacct aaacaccgac tccctataaa agagtggcca ctaaagggaa gatatggaaa
     5341 ccaggtgcta gaagcttttt gaattaaggg cgaattctgc agatgggctc gagatctgcg
     5401 atctaagtaa gcttggcatt ccggtactgt tggtaaagcc accatggaag acgccaaaaa
     5461 cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg gagagcaact
     5521 gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca
     5581 tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt tggcagaagc
     5641 tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct
     5701 tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa
     5761 cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc ctaccgtggt
     5821 gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat
     5881 ccaaaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac
     5941 gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtgc cagagtcctt
     6001 cgatagggac aagacaattg cactgatcat gaactcctct ggatctactg gtctgcctaa
     6061 aggtgtcgct ctgcctcata gaactgcctg cgtgagattc tcgcatgcca gagatcctat
     6121 ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg
     6181 ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta
     6241 tagatttgaa gaagagctgt ttctgaggag ccttcaggat tacaagattc aaagtgcgct
     6301 gctggtgcca accctattct ccttcttcgc caaaagcact ctgattgaca aatacgattt
     6361 atctaattta cacgaaattg cttctggtgg cgctcccctc tctaaggaag tcggggaagc
     6421 ggttgccaag aggttccatc tgccaggtat caggcaagga tatgggctca ctgagactac
     6481 atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt
     6541 tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca
     6601 aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa acaatccgga
     6661 agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg
     6721 ggacgaagac gaacacttct tcatcgttga ccgcctgaag tctctgatta agtacaaagg
     6781 ctatcaggtg gctcccgctg aattggaatc catcttgctc caacacccca acatcttcga
     6841 cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt
     6901 tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt
     6961 aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct
     7021 taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg
     7081 aaagatcgcc gtgtaattct agagtcgggg cggccggccg cttcgagcag acatgataag
     7141 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     7201 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     7261 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     7321 aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct
     7381 tgagagcctt caac
//
