LOCUS       sequence-177608-       10701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression in S.cerevisiae of 4CL5 and AtSDT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10701)
  AUTHORS   Eudes A, Mouille M, Robinson DS, Benites VT, Wang G, Roux L, Tsai 
            YL, Baidoo EE, Chiu TY, Heazlewood JL, Scheller HV, Mukhopadhyay A, 
            Keasling JD, Deutsch S, Loque D
  TITLE     Exploiting members of the BAHD acyltransferase family to synthesize 
            multiple hydroxycinnamate and benzoate conjugates in yeast.
  JOURNAL   Microb Cell Fact. 2016 Nov 21;15(1):198.
  PUBMED    27871334
REFERENCE   2  (bases 1 to 10701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2016-11-21"; volume: "15(1)"; pages: "198"
COMMENT     Sequence Label: pDRf1-4CL5-AtSDT (JBEI-11535)
FEATURES             Location/Qualifiers
     source          1..10701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      568..1023
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(655..674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     865..886
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1053..1069)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1053..1069)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1066..1088)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1077..1093
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1101..1107,1108..1125,1126..1131))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1146..1167
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1284..1301)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1455..2043)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1535..1554)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2214..3005,3006..3074))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2837..2856
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3075..3179)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3247..3265
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3290..3505
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3506..4309
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      4420..5766
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     primer_bind     6187..6209
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6201..6218
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6202..6218
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(6231..6478)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     6444..6462
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      complement(8631..8818)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     8739..8763
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     8966..8985
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8966..8984
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9047..9071
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10437..10461)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttagacgata atgataggac atttgaaaat aatacaatat ttggcctttt tatataatcg
       61 aaactaatgg aggggagcat aagcggtacc caccatcgac cacgtttcgt caccggtcat
      121 aattggaata aacaaaacaa acaaacgaac taaagagtgt gagagtaaca atttccacag
      181 aggtaaaaaa aaaaaaaagg aaaacagaaa caaaaaaagc tcatagttct actatagctt
      241 cttcagactt tctgtgaacg agggaaaaag acaacaaaaa agcaatggat aaacggtctc
      301 gatcgaggcc tggaagtgcc gcattaggaa atacagatgc aatgcgcgcg agtttacgaa
      361 attctcgcag gagtttctcc ggccatgtgc ggctggttct acgtctcgtg tatgggttta
      421 tttctgcggt aattatcgcc ggatctccgc ttcgagaccg ggtttgtttt caaattaccg
      481 gaaaaggtca ggcaaaacag agtgatgtat gggtgatccc agcaatgcct gtttatgttt
      541 ctccgtttca ggaagcttgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
      601 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      661 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      721 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
      781 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
      841 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
      901 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
      961 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     1021 tttcctgatg cggtaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     1081 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     1141 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     1201 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     1261 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1321 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1381 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1441 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1501 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1561 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1621 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1681 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1741 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1801 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1861 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1921 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1981 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2041 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2101 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2161 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     2221 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     2281 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     2341 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     2401 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     2461 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     2521 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     2581 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     2641 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     2701 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     2761 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     2821 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     2881 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     2941 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3001 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3061 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3121 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     3181 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     3241 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattagcttt tcaattcatc
     3301 attttttttt tattcttttt tttgatttcg gtttccttga aatttttttg attcggtaat
     3361 ctccgaacag aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat
     3421 atgtagtgtt gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa
     3481 aacatgcagg aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac
     3541 tcatcctagt cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt
     3601 gtgtgcttca ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg
     3661 tcccaaaatt tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg
     3721 cacagttaag ccgctaaagg cattatccgc caagtacaat tttttactct tcgaagacag
     3781 aaaatttgct gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat
     3841 agcagaatgg gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg
     3901 tttgaagcag gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga
     3961 attgtcatgc aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc
     4021 gaagagcgac aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga
     4081 tgaaggttac gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagacgc
     4141 attgggtcaa cagtatagaa ccgtggatga tgtggtctct acaggatctg acattattat
     4201 tgttggaaga ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag
     4261 aaaagcaggc tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt
     4321 ataagtaaat gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt
     4381 attacccaat tctcatgttt gacagcttat catcggatcg atccaatatc aaaggaaatg
     4441 atagcattga aggatgagac taatccaatt gaggagtggc agcatataga acagctaaag
     4501 ggtagtgctg aaggaagcat acgatacccc gcatggaatg ggataatatc acaggaggta
     4561 ctagactacc tttcatccta cataaataga cgcatataag tacgcattta agcataaaca
     4621 cgcactatgc cgttcttctc atgtatatat atatacaggc aacacgcaga tataggtgcg
     4681 acgtgaacag tgagctgtat gtgcgcagct cgcgttgcat tttcggaagc gctcgttttc
     4741 ggaaacgctt tgaagttcct attccgaagt tcctattctc tagctagaaa gtataggaac
     4801 ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac
     4861 gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca
     4921 aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct
     4981 ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt atctctagta
     5041 ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc gaaaacaata
     5101 cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga aaccgttcat
     5161 aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa agtatgcgca
     5221 atccacatcg gtatagaata taatcgggga tgcctttatc ttgaaaaaat gcacccgcag
     5281 cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa
     5341 tagacaccaa agtagccttc ttctaacctt aacggaccta cagtgcaaaa agttatcaag
     5401 agactgcatt atagagcgca caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa
     5461 aatagcgctc tcgggatgca tttttgtaga acaaaaaaga agtatagatt ctttgttggt
     5521 aaaatagcgc tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga ttctttgttt
     5581 gaaaaattag cgctctcgcg ttgcattttt gttttacaaa aatgaagcac agattcttcg
     5641 ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt
     5701 tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa gcacagatgc
     5761 ttcgttaaca aagatatgct attgaagtgc aagatggaaa cgcagaaaat gaaccgggga
     5821 tgcgacgtgc aagattacct atgcaataga tgcaatagtt tctccaggaa ccgaaataca
     5881 tacattgtct tccgtaaagc gctagactat atattattat acaggttcaa atatactatc
     5941 tgtttcaggg aaaactccca ggttcggatg ttcaaaattc aatgatgggt aacaagtacg
     6001 atccgattat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     6061 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     6121 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     6181 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attggcatgc gcaaattaaa
     6241 gccttcgagc gtcccaaaac cttctcaagc aaggttttca gtataatgtt acatgcgtac
     6301 acgcgtctgt acagaaaaaa aagaaaaatt tgaaatataa ataacgttct taatactaac
     6361 ataactataa aaaaataaat agggacctag acttcaggtt gtctaactcc ttccttttcg
     6421 gttagagcgg atgtgggggg agggcgtgaa tgtaagcgtg acataactaa ttacatgact
     6481 cgagcggccg cctatttaga gcacatggtt tccaatttag ctcgcagatc cttcctcaga
     6541 atcttgcccg aaactgcttt tggtataact tctatgaaaa acaccatctt aatccgcttg
     6601 tagtgaacca cctgtttgtt tacgtaactc ttgacatcat cttcagttaa ctgagaccct
     6661 tgagatcttg ctacaaatgc tactggaacc tcatcagcta cttcatcctt cattgcaaca
     6721 actgcggcat catcgataga aggatgagaa ataagcaatg cttcaagctc agctggagcc
     6781 acttggtagc ctttgaattt gatcagttcc ttcaaccgat caacaatgaa gatctcatca
     6841 tcatcatcca caaacccaat atctcctgta tgtaaccacc cgtctttgtc tatggttcgt
     6901 gcagtagctt ccggatcatt caaataacct ttcatgagct gatgacctcg gacgcagatc
     6961 tcgccagatt tgttgcgcgg caaggagatt ccggtttcgg tatcgaccac tttcatctcg
     7021 gcgtttctga tcacagtccc acatgcaccg gacttggttt taaatgggtt ctttgcaaac
     7081 gctaatgact tagctaccgt tccggactcg gtcattccat aaccctgacc aaaaatggca
     7141 ttaggaaact tgagtcgcac ggcgtcttca agctccttct ttagtgtagc tgcacctgat
     7201 agcataattc tcaccgagct caagtcgtat ctctccgtct caggggattt aatgaatgct
     7261 aaaaccaccg gaggagccac cggcacaacc gtgactttgt acctttgaat cagctccatc
     7321 accagattca actcgaacct cggcacgatc agtaacgctg caccggttct catcgccgaa
     7381 agcatcaacg cgtcgagagc gtaaatgtga aacattggga gaaaacagag gatgacgtca
     7441 ttggccgtga agttaaggtt agggttttct ccgtcgactt tctgagcgat gcttgtaact
     7501 aaacccttgt gagtaatcat cactcccttt ggaagtcccg ttgttccgga agagtacggc
     7561 atcgccaccg tgtcttccgg cgatatttta ggttttagaa gttctgtttc gtccgcttga
     7621 gtcagttccg tgaaactcac gcaaccatca tcactcgacg aaacaacgcc gttgtcaccg
     7681 tcatcgtcta ggcaaacgat caaaacgccg tcgtttttga ggtttgttag tttatcgact
     7741 aaacatttct tcgtgatgat catcttcgcg gcggaggctt tagcctgttt agcgatctcc
     7801 ggctgagtgt agaacggatt cgcggtagtg gaaacggctc caaggtaagc aacggcaaga
     7861 aaagacagag caaactccgg cgagttcggg aggagaagca ttacgacgtc tccgtggcgg
     7921 atcccgagcc ggtggattcc ggcagctatc ctccgcatgt tggtttgtac atcggcgtag
     7981 gtcaaaatac ggccagtggc accgtcgatg atacaggttg ttgaggaatc tccgtcgcca
     8041 tcgccggaga acctttggaa gacgtaatcg gtgagaggga ggtggttagg gatgaagata
     8101 tcaggaagct tagatcggaa aatgaaatca tgagaaggtt cttcttcttc gtcttcttga
     8161 tctatcttct ttgtgagaaa atgcgtttgt tgttggagca ccatgcggcc gcccggggga
     8221 tccactagaa ctttttgatt aaaattaaaa aaactttttg tttttgtgtt tattctttgt
     8281 tcttagaaaa gacaagttga gcttgtttgt tcttgatgtt ttattatttt acaatagctg
     8341 caaatgaaga atagattcga acattgtgaa gtattggcat atatcgtctc tatttatact
     8401 tttttttttt cagttctagt atattttgta ttttcctcct tttcattctt tcagttgcca
     8461 ataagttaca ggggatctcg aaagatggtg gggatttttc cttgaaagac gactttttgc
     8521 catctaattt ttccttgttg cctctgaaaa ttatccagca gaagcaaatg taaaagatga
     8581 acctcagaag aacacgcagg ggcccgaaat tgttcctacg agctcgcatg ccggtagagg
     8641 tgtggtcaat aagagcgacc tcatgctata cctgagaaag caacctgacc tacaggaaag
     8701 agttactcaa gaataagaat tttcgtttta aaacctaaga gtcactttaa aatttgtata
     8761 cacttatttt ttttataact tatttaataa taaaaatcat aaatcataag aaattcgctt
     8821 atttagaagt gtcaacaacg tatctaccaa cgatttgacc cttttccatc ttttcgtaaa
     8881 tttctggcaa ggtagacaag ccgacaacct tgattggaga cttgaccaaa cctctggcga
     8941 agaagtccaa agctggatcc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
     9001 gccccccctc gaggtcgacc tgcagcggcc atcatgaatt aactacacca ctttgtacaa
     9061 gaaagctggg tccacttatt ccaaggcctc catttcctct ttgaacttga ccattgcgat
     9121 ccttggcaaa gtaacgacaa tctgaacacc tccgaccata tcagattcca atctagatgg
     9181 cctcatgaac atgttaactg tccttgcagt ctcagatgga accacaggaa cgatattaac
     9241 tggctcatcc caaccgaagt ccattgatcc gaaaataccg atgtttctcc aatcggttaa
     9301 acagaacaaa ccgtctttct tacccttaat agttaaattc atcttgatga ttttttctgt
     9361 attggctaat tcttcttgca aataatcctt atcgtgagcg ttcctcttgg cctctttaat
     9421 caacttcacg atgtcactaa tggtgaattc ctcaacttct ctagcagtta aaggaacata
     9481 catatcaatg taggcgttac cataataacc gtcaggaagt ggaggatcca cgacgttacg
     9541 gataccgact gataaaccca aaacagtcac accgtctcta tctaaattca atgctttgac
     9601 cctggacttc cataaataag caccgatgac ctcgaaagta gtgattgtct cattagaaaa
     9661 gtcgtactct ttcaaggtgg cctccttcaa tcttctaata gagtcggccc tgatggtaat
     9721 cttttcagta acccaatcgt ctgttggtaa atatggactt gcggcggtat cgtcaccagg
     9781 aacgaaagga ggttgatcgt cttcaggttt tccgaccaac ctttctctct cccaaatagg
     9841 agtcaccata ggcttctttt taccaccagc caaatctgtc aatgcacaca tgatatgtcc
     9901 ctcaccataa ccatcacaca ttgcatgaga catggccata cccaaaatga aaccaccaca
     9961 ctcaaacttg gtgacctgta atgcgaaagg acggtaaccg tcgatatcaa cgtgcaagac
    10021 tggtaaaaaa ttcaaggctg tgtcggaatc gatgttttct aagtttttca aagaactcaa
    10081 ttcgacgtta gcagtggcaa ctgtgaaagg aacaccacct ccatcaccac cacaagataa
    10141 ttgtaacttc ctatcagatt cttgtctttt caaagaaccg gataaaggat aataataacc
    10201 taataagtcg gacaacgcct ctcttaacaa ggcttcaggt ctattatcgt catcagcgac
    10261 attttttgcc ttaaacacgt agcaagcctt ataaatgacc tcgttataag ggtcgttatc
    10321 taatgtagat aaattaaggg tttgctgagg gatatgtttt gaaggcttaa ccatctcggt
    10381 caacattttt tcaaccatca atgggattga agatccaatg tggattggca tgtggaagcc
    10441 tgcttttttg tacaaacttg tgatggccgc gaattcacta gtcgaggctg gggtatattt
    10501 tttttctttc ttttgaatgt gtgtataaaa gagagaaaaa atggttattt tccaattaat
    10561 taattattaa cgattgttaa agaaaaaatt tatcaacgag gttgatagaa aaaaaaagtt
    10621 tttgtagata tagaagaaaa aaagattttc actattggtg ttataggaaa gaaagagaaa
    10681 aaatttaaga gaaattagat g
//
