LOCUS       sequence-177616-        2335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  jStack yeast assembly into plant vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Shih PM, Vuu K, Mansoori N, Ayad L, Louie KB, Bowen BP, Northen TR, 
            Loque D
  TITLE     A robust gene-stacking method utilizing yeast assembly for plant 
            synthetic biology.
  JOURNAL   Nat Commun. 2016 Oct 26;7:13215. doi: 10.1038/ncomms13215.
  PUBMED    27782150
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-10-26"; volume: "7:13215. doi"; pages: " 
            10.1038/ncomms13215"
COMMENT     Sequence Label: pms5102(JBEI-10536)
FEATURES             Location/Qualifiers
     source          1..2335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(244..1035,1036..1104))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     867..886
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1105..1209)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1277..1295
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1299..1342)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1363..1611
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(1649..1666)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(1820..2335,1..73))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1900..1919)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
       61 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
      121 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
      181 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
      241 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
      301 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
      361 ccccagtgct gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat
      421 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
      481 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
      541 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
      601 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
      661 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
      721 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
      781 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
      841 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
      901 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
      961 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     1021 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     1081 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     1141 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     1201 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     1261 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
     1321 agctttcgct aaggatgatt tctggaattc ggtctcagtc catatgaaga tgaagatgaa
     1381 atatttggtg tgtcaaataa aaagcttgtg tgcttaagtt tgtgtttttt tcttggcttg
     1441 ttgtgttatg aatttgtggc tttttctaat attaaatgaa tgtaagatct cattataatg
     1501 aataaacaaa tgtttctata atccattgtg aatgttttgt tggatctctt ctgcagcata
     1561 taactactgt atgtgctatg gtatggacta tggaatatga ttaaagataa gggagtgaga
     1621 ccggatccta actcgagctg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1681 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1741 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1801 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc
     1861 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1921 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1981 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2041 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2101 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2161 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2221 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2281 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaa
//
