LOCUS       sequence-177627-        9874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complements mutations in Aspergillus nidulans gene choA (AN2154) 
            (mutants require choline).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9874)
  AUTHORS   Dohn JW Jr, Grubbs AW, Oakley CE, Oakley BR
  TITLE     New multi-marker strains and complementing genes for Aspergillus 
            nidulans molecular biology.
  JOURNAL   Fungal Genet Biol. 2018 Feb;111:1-6. doi: 10.1016/j.fgb.2018.01.003.
            Epub 2018 Jan 5.
  PUBMED    29309843
REFERENCE   2  (bases 1 to 9874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2018-02"; volume: "111:1-6. doi"; pages: " 
            10.1016/j.fgb.2018.01.003. Epub 2018 Jan 5"
COMMENT     Sequence Label: pLO102
FEATURES             Location/Qualifiers
     source          1..9874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        133..151
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     133..150
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(223..234)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6656..6675)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6657..6675)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6682..6699)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6682..6698)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6691..6713)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             6836..7138
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7017..7036
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             7487..8281
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7487..8281
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7541..7560)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8151..8170
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     regulatory      8481..8490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8487..8861
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      8999..9587
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9488..9507
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9741..9758
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag ctatttaggt gacactatag aatactcaag ctatgcatca agcttggtac
      181 cgagctcgga tccactagta acggccgcca gtgtgctgga attcgccctt caatgctctt
      241 caccctcttc tgccaaggtg ggactgtaga tggtgaatta acgtcatgga ttagtaataa
      301 tagacaacgt ggtggagtgg ttaacacgca gccctgctat ttcgtaaggc tgtctcttag
      361 gaggcgtagg ttcgaatcct gccgttgtcg catatttttg tttgcccaac gcatcgccat
      421 gtcatggtag gaggttattc cgtacagtgg gagcaggttg attttgacgg gagggcagac
      481 tggaaaagct ggcgacacaa ttttgccaac cagaacctac ctaacaacat caatcggtat
      541 gcagtttatc gtgtatggta atttcggttc aacggccttc gcacgaacaa gcatagcatc
      601 ggagaaattc cccggaagtc tcgtttctta actatccggt tggagcggcg tcgacattct
      661 tttactacta tggggtatgc agagtaacag ttctctttaa acggtaggcg gtctagtgcg
      721 gcatgccaat cgtccgacct gaagccccta cccctacaat tgcttaagca ctcattttgc
      781 ctacgtaatg acaacgtggt ggagtggtta acacgcagcc ctgctaaggc tgtctcttag
      841 gaggcgtagg ttcgaatcct gccgttgtcg cgaaaccttt ttgcgtctat gctttagccc
      901 tccttttttt ttgtcgcctc ttaccgctgt agagaacaga attactacgt atgccatgtc
      961 caaccttcgg gtagatgcgg actaagctag gtagctatct cagggcttgg tttcctgccg
     1021 gttgttcttg cgttgtttaa tacgcagaat aagatgcatt attcgtagcg tgctatgaca
     1081 ttactagttg cccttctctc tatttccgtc tcattcttcg ctgtagccct catggctgct
     1141 ctgattggcg ttttccgact caacggagcc ccgtttctct gccccagcgg aggggggttc
     1201 cactccgaga gtcggcgaga atagttacct gtacgcagca gctagagagc acttgaatgg
     1261 atagctgact gcgcgattac cgttaggtag tatgagttac ttacggtgta gtcccttatt
     1321 gatacccgta ccgactaggt aggggattgt ggcattttgg tcattacggt atggtgctaa
     1381 aataaacacg ggcctgtctc ggttgtccct ctcgatgtaa atcgtcaaca cccctccttc
     1441 tcccctgttt tctgcaacaa gttttcctcc tcgtcctctc ctcttcttcc tctcgcccgc
     1501 catatttaat ccccttctgg ttcccctttc cctctgtctt gaccatcgca gttttcaact
     1561 ttcaaagaag tcgaattcga cataccacca cggccgcggc taactaaatc gggactggcg
     1621 tctcacatga cgtaaaccaa tcaagatctg gccgcttaag taccccaacc ctttccggct
     1681 gtcacatgct ggtgacctcg tggttgaatc ctggtcgtgt ttttggcttt gattccccct
     1741 ctctttcttc atcaacccca aaccacaatc atggatcgtg gcctttcaac aggcactcac
     1801 aatgataacg aggggcttcg ggagagaccc gttgcctctc aatcggcttc aacgctcagt
     1861 cccgaggctc ttaccgccac tggtgacgta gagccgaagg acactgggaa ggaattgaag
     1921 acctatggac gcaccccgga tggcacaggt aagtccttgc tctgtacatc tataaatctg
     1981 ttgggccggt caattttgtc gcctctgcgg cgtttggctg gcatcctcac ctatactcag
     2041 tgctaaccat gatggattct cagtatttac tgtgccccaa acacatgaca tggtttccca
     2101 gctcctgtcg ccttcagagc cgaagaacct atcagatatc atcgtcctgg ctatcttggg
     2161 tgctcatatt ctccttatct gggcgctgcc caaaggtgcg aaggttcctg tctctgcggt
     2221 cttgtatctc ttctggcgtg cgggctacaa tgcggggatt ggatggcttc ttcacaacca
     2281 atcccaccac aaaaccctcg ttcgctgggc tgagaaaacc aagatcttcg tcaatcctgc
     2341 gacgggacag aatccgcacc ctcatctgta caacctgatc aaacgggaac tggaaaccaa
     2401 gatcccccac gactattctt tcgagaaggc acctctcgag tacaacacct ggctcgtttt
     2461 taggcgcctg gtcgatttga tcttgatgtg tgactttaca tcgtactgtc tctttgcgat
     2521 agcctgtagt caccatcctg tcaatgagag cctgctaatg accgtgctga ggtggttctc
     2581 cggcattgtg ctggtgctct tcaacctctg ggtcaagtta gacgcgcatc gtgtggtaaa
     2641 ggactatgcg tggtactggg gcgacttctt ctacctgatc gaccaggaat tgacattcga
     2701 cggtgtcttc gagatggcgc cccacccgat gtactcggtt ggttacgcgg ggtactacgg
     2761 tatctcgcta atggccgcta gctacaaggt gctgttcatc tctatcatcg cccacgcagc
     2821 tcagttcgct ttcttggtct tcgtcgaaaa cccacacatt gacaagacgt acaatccccc
     2881 accaccccgc aaacgtacaa tcgaccagga tacggcttcc acgactaccc acaccacgga
     2941 ctctcccatc gcgccagcac ccgttgatga aaatgtgccc cacgcaccaa cgttttcaag
     3001 ccggcctcct ccatcggtcc acaatctgtt aggcttccac aatttggatc tgtatcgcat
     3061 aaccgatact tcgtccatgc tggtccaatt cctagtattc gctgtcactg tccttacgcc
     3121 ctccacgccc tggtatcagt tcctgttcgt ggccaatgca gcggtgtgga ggctctggta
     3181 ctctgttggc attggatacc tcctcaatcg ccaatccaac tgcaaagctt ggacccggca
     3241 tttcgtcaag tatggcgaaa caccccatga ggcgtggaac cagtggaagg gcacgtatca
     3301 cctaagcatg atcatgtgct acgcgagctt cattgctgct gtatggaaaa tgtatactct
     3361 cccctccgac tggggctatg gccttgtctt actccggcac gttctcgggg cagggctgat
     3421 ctgcctacaa atctggacgt ccgttagtat ctatgagtcc cttggagaat ttggctggtt
     3481 ctacggagac ttcttctacg acgagtcgcc caagttgacg tacaacggga tctatcgctt
     3541 cctgaacaac ccagagcgag tccttggtct tgccggggtc tggggtgcgg tccttatcac
     3601 tagtagtgga gcaatcacgt tcctcgcgct tctgagccac atcctcagcc tggcgttcat
     3661 tcagttcatt gagcgtccac acatgcaaaa gctctatggc cagagtatcc gtcgcgatgc
     3721 cggtcttgtt aagaacctga aacggtcctt gcctcctcct ctcaagcaac ttcatgggag
     3781 tatggacaaa atcgtcgacg gttctttcga gtttatagag gatttcttgg aaaatgcccg
     3841 ccccaagctt gctgccggtg ttgacacgtt cgtgaaggat acaaccgccc tcttccagaa
     3901 gtaccccgcc cgtgttacca tcacccgcat cgatgaggac gtggccggat atgattcgcg
     3961 agattactct ctggaggttg aaggcaccga ttcatcagct cttgccgaat gcgaccaaag
     4021 tagtggtaga gagggtgcta atgcccgcat gccccttgat agacgcggtg atctgaaaaa
     4081 cttggtattc gaatacggag ccccgatcaa ggtcaaatgg actgcgcctc tcaatcacag
     4141 caagaaagat tggattggac tttatcgcgt gaccgataac acttcccggg agatcacaaa
     4201 ggtttcttcc caaggacgat ggatcgcggt gaatgaagga tcctacgaca atttgacctg
     4261 cgaaaagggg attgtttcga gcgatgtggt catcccagct ccgcatgggg acaaccgcga
     4321 gatggcctcg ggggaagtca tcttctccag cgacaagcta ttctggaccc aaggcgtctt
     4381 cgagttccgg taccaccaca atggcaagca taacgtgatg gcgatctcaa gaccgtttga
     4441 aatccgcatc cgccggtttg aggaagaagg ccaccatgag atgatgcaga cctctgttga
     4501 aaatagtctg ttgccagtcg ttcgcaactg ctttgaccga gacccagagg tcgcgcctga
     4561 gaccgtcgac gaacagtttg gatccctggt cgaacgtgac ggcaagtatg ccaagcgggt
     4621 tgtttttgcc gtgcatcaga tgtaaggctt cctattttcc tatcataata tcgtctaccg
     4681 agtgcatgtt aacacggtgt tcaggttcgg catcgaattc gccccggaag tcgtccgcgc
     4741 cgatggaact gtacgcaacc ttgcttggcg aatatgcaat gcaaagaagg ttttggtaag
     4801 tttttctcca cgtataaatc accagcgtgg cttctaataa ctgctcctat gacaggcgcc
     4861 ttacagtatg tcgcgcaacg gtgcgtccac cccgaccgaa agaaaagaat aaagcggata
     4921 cgcctacacc ttgcgtgtcc tccctcccta ttgaacaaat gctatcacga cgatttgctt
     4981 ctctgctgtt tctttgaata tgagccacga aagcttttca tagataggtc ttagatgctg
     5041 cgtgtctgtt tgtcgcatcg gtcgttttct ttcctttcac ctgccttgat cacatacgat
     5101 tgcttttgaa cccgaatggt gaagctacat ggcgaataaa atccgaatag atggagacgg
     5161 atgggatgtg gagaagatgg ttgcctgcag gggtttcctg atgagacctg atgttgtgag
     5221 cttctgccgg agcttgctgg ggggtatgta aagtactaca ttgagcttcg tggtatatat
     5281 tatgaatgaa ttatcgtttt ctagataaag atatagttcg tagaatggct atgtttcatt
     5341 tccaagttat ctacctaggg gtactatatg aaagctgaca cgaatctaca aagacagtta
     5401 gcataagctc gcaatcctgt cgagtggtag aagccagaaa ccacctgccg ccagatagaa
     5461 ggcgaccggt agtatagcga aaggatcagc caagccggta aattcatccc atcccctgcc
     5521 aatttcgacc cccccgcgag ccatccagga cggacgacaa gccgagaaac cgacaaagac
     5581 acaagtcaag cacagcaaca cgcagccagg aacaaacgcg gaagacacaa cgagggagcc
     5641 cgccacccaa cgctccacaa ccatcctcca tttcatcgac cgtgtcttcc gagaaagaca
     5701 tggacgacga aatgtccagt acaagggagg tgggcaagcg gatcgttgca cttcaagtgc
     5761 gatgtgtgga agccgatgca ggctcgaggg gcggtttctc cgaagagaat cattgaatgg
     5821 attgacttgt tgtcatcatc aaggagtggt gtgagggagg attgtctgcg tggtagttca
     5881 tacctatgct agtgtgctag tggctgacgt gatgcgagct gagactacga tatctgtatc
     5941 ttgaagctgt tagcttgggt cggcatgggt gatgtgattg tgtcgtgatg aacaagaagg
     6001 gagtttccat atcagtagat ttggtaaggt atttggtttt cttcaatgag tttgcagaag
     6061 cagaactgta aaaaatgttt ctcatcatgg aaacgggtgg gggaaagacg aggccgaaaa
     6121 gaaaaggaat aaaaggcata cctttcgaag agtctacagg tttgatgtga aatagccact
     6181 gagagctcag ctacctgtct gatattaagt tagccaacag ttcccaagaa caggctaaga
     6241 taaaagtgag atgagaaagc attttcagat ggtagtgcat gttgttcacg gcccatttgt
     6301 ttgtcgccga actaagattt ttggatcgtt ttcctcatag aatgcattgt gcggtggaag
     6361 cgatgatggg acaacttaca gtaaaggtta gtatcctgga agagaagaaa aggtgctaat
     6421 gtagctggat tgagccttcg tcattaaaag gtcaattcgc cagagataaa gcggatgttg
     6481 tgtggaagag aagcgaggaa gagaagaaag gaggaggaga agaaaaagag gaaaaagtga
     6541 gaggaaaaac tagcgccgac tccacacagt gcctcctctc agacagaagg gcgaattctg
     6601 cagatatcca tcacactggc ggccgctcga gcatgcatct agagggccca attcgcccta
     6661 tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     6721 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     6781 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctatacg tacggcagtt
     6841 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
     6901 tattattgac acgccggggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
     6961 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat
     7021 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct
     7081 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
     7141 gtcaggcatg agattatcaa aaaggatctt cacctagatc cttttcacgt agaaagccag
     7201 tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga
     7261 aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga
     7321 ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa
     7381 ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg
     7441 caggggatca agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     7501 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     7561 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     7621 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     7681 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     7741 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     7801 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     7861 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     7921 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     7981 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     8041 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     8101 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     8161 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     8221 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     8281 aattattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     8341 ttcacaccgc atcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8401 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     8461 aataatagca cgtgaggagg gccaccatgg ccaagttgac cagtgccgtt ccggtgctca
     8521 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
     8581 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
     8641 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
     8701 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
     8761 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
     8821 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta aaacttcatt
     8881 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8941 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9001 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9061 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9121 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9181 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9241 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9301 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9361 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9421 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9481 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9541 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9601 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9661 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9721 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     9781 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     9841 ccgactggaa agcgggcagt gagcgcaacg caat
//
