LOCUS       sequence-177642-        6811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expressio of EGFP tagged rat Syntenin1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   Koroll M, Rathjen FG, Volkmer H
  TITLE     The neural cell recognition molecule neurofascin interacts with 
            syntenin-1 but not with syntenin-2, both of which reveal 
            self-associating activity.
  JOURNAL   J Biol Chem. 2001 Apr 6;276(14):10646-54. doi: 
            10.1074/jbc.M010647200. Epub 2001 Jan 4.
  PUBMED    11152476
REFERENCE   2  (bases 1 to 6811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M010647200"; journalName: "J Biol Chem"; date: 
            "2001-04-6- 6"; volume: "276"; issue: "14"; pages: "10646-54"
COMMENT     Sequence Label: pEGFP-rSyntenin1
FEATURES             Location/Qualifiers
     source          1..6811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..48)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     83..102
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(120..575)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(257..278)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     469..488
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        602..706
                     /gene="bla"
                     /label=AmpR promoter
     promoter        708..1065
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        708..899
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(731..751)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      916..1051
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1100..1894
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1100..1894
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1154..1173)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1764..1783
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2082..2101)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2126..2173
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      2502..3090
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2991..3010
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        3265..3568
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3569..3772
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3723..3743
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3811..3820
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3817..3819,3820..3822,3823..4533)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3817..3819,3820..3822,3823..4533)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3862..3883)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4123..4142)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4470..4491
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    join(6803..6811,1..113)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
       61 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta
      121 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
      181 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
      241 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
      301 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
      361 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
      421 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
      481 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
      541 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
      601 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      661 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
      721 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
      781 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
      841 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
      901 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
      961 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     1021 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
     1081 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     1141 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     1201 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     1261 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     1321 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     1381 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     1441 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     1501 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     1561 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     1621 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     1681 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     1741 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     1801 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     1861 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     1921 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     1981 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     2041 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca
     2101 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt
     2161 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     2221 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     2281 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
     2341 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2401 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2461 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2521 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2581 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2641 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2701 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2761 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2821 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2881 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2941 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3001 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3061 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3121 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3181 gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt
     3241 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     3301 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     3361 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     3421 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     3481 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     3541 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     3601 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     3661 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     3721 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
     3781 gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg
     3841 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3901 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     3961 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     4021 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     4081 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     4141 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     4201 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     4261 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     4321 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     4381 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     4441 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     4501 gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatctcg aggatccgaa
     4561 ttcgcggccg cgtcgacagc ggcaggaaca tcgcggtgtt cggggcagaa aacacctgca
     4621 aaaatgtctc tctatccatc tcttgaagat ttgaaggtag acaaagtaat tcaggctcaa
     4681 actgcctctt ctgctaatcc tgccagccag gccttttttt taaatgtggt cgattctgct
     4741 gcctttcctc caggtggaaa tctctatcct aaactgtatc cagaactctc tcaatacatg
     4801 ggactgtctt taagtgaagc tgaaatatgt gaaaacatgc ctgtggtctc tggagcgcca
     4861 acccaagggc agttggtagc aaggccatcc agtgtgaact acatggtagc acctgtgaca
     4921 ggtaacgatg cggggattag gagagcagag attaagcaag ggattcggga agtcattttg
     4981 tgtaaggatc aagatggaaa aattgggctc agactgaagt cagtagataa tggtatattt
     5041 gttcaactag tccaggctaa ttccccagcc tccttggttg ggctgcgatt tggggaccag
     5101 gtgctccaga tcaatggtga aaactgtgcc ggctggagct ccgataaggc acacaaggtg
     5161 ctcaagcagg cctttggaga gaagattacc atgaccattc gtgatagacc ctttgaacgg
     5221 acagttacca tgcataagga cagcagtgga catgttggct ttatctttaa aagtggaaaa
     5281 atcacatcca tagtgaaaga cagttctgct gccagaaacg gccttctcac tgatcaccac
     5341 atctgtgaga tcaacggaca gaacgtcatt ggcttgaagg atgctcagat tgcagatata
     5401 ctgtcaacag ctgggactgt ggtcaccatc acgatcatgc ctgcctttat cttcgaacat
     5461 attattaaac ggatggctcc cagcattatg aaaagcctga tggatcacac cattcctgaa
     5521 gtttaaacct tgtacagtgc atcaccgccg aacgcttcca gtcttcctct tcggcaactt
     5581 ctctatttca ccgcacatga agctttcccg gatccaggga gactgtgctg catggagacc
     5641 tgtctatctt ccaccatggc tgggaacctc actctatctg agagcgtctt cagattttaa
     5701 gataggtgta gccttatcct ggttttacag atgtgaaact ctcaaaagat tccctgactt
     5761 tctagacttg gttctttact gggaacctga tgtttttcta taagccattg tgattaggat
     5821 gactggtgta ggcttagctg tgtccgagaa ctggtctgct ttctccgaga agtgcacagt
     5881 gctaggcacg tgcttcagtt ctgtggcata tgtggattgt agtacatcag catagtacct
     5941 gtaggatgat tgcctttgat cgctactttt taagtttgtg tgtgagtgtg taaaattcca
     6001 gttaatcaac acttccattc cacaaaagca ttatacagtt ctgtgtaggt ggcaagagac
     6061 taatgattgt cactaaattc taactacctt cacttaatgt gcttcagttg tcgccttaac
     6121 tacagtaagc atttagaata aaagccaaaa tagaattatt gctgccttct gaaacctgag
     6181 aggttagctc tctatcaagc tgttctatgc tagccctgag cagcttactg gtgtcgcaga
     6241 gctgcctagc tttaccaaga ttttctagtc actgcataaa ggaaacgcct aagagttgcc
     6301 gtattgcttt ctgagataca aattgctata tatttcatgt aaaagttgtt atactaaata
     6361 ggataaattt tggagtctct ttttcactcg tagattaagt gggtaatatc taatttggca
     6421 cttgagtcct aacattatgt aactttagct aggttggggt gggtttagaa tgtttcccta
     6481 gtcatctcag tttgttccat tttctgactc tatcctgcaa acaaaaatgg caatgacact
     6541 ttatcctgac tctttccttc ttagtttgtg cctatcttat tattttatgt gatattatcc
     6601 tattaaatgt ataaaaatat taaatgtcaa aaaaaaaaaa aaaaaaaaaa aagtcgacgc
     6661 ggccgcgaat tccagatcca ccggatctag ataactgatc ataatcagcc ataccacatt
     6721 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     6781 aatgaatgca attgttgttg ttaacttgtt t
//
