LOCUS       sequence-177643-        5021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human Syntenin2a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Koroll M, Rathjen FG, Volkmer H
  TITLE     The neural cell recognition molecule neurofascin interacts with 
            syntenin-1 but not with syntenin-2, both of which reveal 
            self-associating activity.
  JOURNAL   J Biol Chem. 2001 Apr 6;276(14):10646-54. doi: 
            10.1074/jbc.M010647200. Epub 2001 Jan 4.
  PUBMED    11152476
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M010647200"; journalName: "J Biol Chem"; date: 
            "2001-04-6- 6"; volume: "276"; issue: "14"; pages: "10646-54"
COMMENT     Sequence Label: pSG5-hSyntenin2a
FEATURES             Location/Qualifiers
     source          1..5021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     83..105
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     97..114
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     98..114
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(151..171)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        259..469
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        281..477
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      328..463
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          508..1080
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1088..1107
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1135..1154
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1135..1153
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2141..2262
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2178..2197)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2232..2251
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2296..2313)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2467..3055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2547..2566)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3226..4017,4018..4086))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3849..3868
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4087..4191)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4259..4277
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4315..4337)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4437..4456
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(4523..4978)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4660..4681)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4872..4891
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
       61 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg
      121 tcgacttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
      181 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaagga
      241 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
      301 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
      361 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
      421 tctgagctat tccagaagta gtgaagaggc ttttttggag gcctaggctt ttgcaaaaag
      481 ctccggatcg atcctgagaa cttcagggtg agtttgggga cccttgattg ttctttcttt
      541 ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt gttgtttaga
      601 atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt ctttcacttt
      661 ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt aactttttcg
      721 ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta tttgtcagat
      781 tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt atattgtact
      841 tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat atttctgcat
      901 ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacat cctggtcatc
      961 atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag gataaaatac
     1021 tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctt tttcctacag
     1081 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgtaatac
     1141 gactcactat agggcgaatt catgtcatcc ctgtacccat ctctagagga cctaaaagtg
     1201 gaccaagcca ttcaggccca ggtcagagcc tcacccaaga tgccagccct gccagtccag
     1261 gcaacagcca tttccccacc accagttttg tacccaaact tggcagaact ggaaaattat
     1321 atgggtcttt ccctctccag ccaagaagtc caggagagcc tgcttcatat tccagagggt
     1381 gacagtacag cggtctcggg ccccgggccc ggccagatgg tggcaccggt aaccgggtac
     1441 agcctgggcg tgcggcgagc tgagatcaag cccggggtgc gcgagatcca cctgtgcaag
     1501 gacgagcgcg gcaagaccgg gctgaggctg cggaaggtcg accaggggct ctttgtgcag
     1561 ttggtccagg ccaacacccc tgcatccctt gtggggctgc gctttgggga ccagctcctg
     1621 cagattgacg ggcgtgactg tgctgggtgg agctcgcaca aagcccatca ggtggtgaag
     1681 aaggcatcag gcgataagat tgtcatggtg gttcgggaca ggccgttcca gcggactgtc
     1741 accatgcaca aggacagcat gggccatgtc ggcttcgtga tcaagaaggg gaagattgtc
     1801 tctctggtca aagggagttc tgcggcccgc aacgggctcc tcaccaacca ctacgtgtgt
     1861 gaggtggacg ggcagaatgt tatcgggctg aaggacaaaa agatcatgga gattctggcc
     1921 acggctggga acgttgtcac cctgaccatc atccccagtg tgatctacga gcacatggtc
     1981 aaaaagttgc ctccagtcct gctccaccac accatggacc actccatccc agatgcctga
     2041 agccactgca gggcagggca ggcagggggg gcttcccgcc ctcctgcagc aaagggcaac
     2101 caccctcgga tgaattcgga tccagatctt attaaagcag aacttgttta ttgcagctta
     2161 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2221 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggtcgactct
     2281 agactcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2341 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2401 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2461 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2521 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2581 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2641 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2701 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2761 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2821 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2881 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2941 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3001 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3061 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3121 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3181 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3241 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3301 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3361 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3421 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3481 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3541 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3601 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3661 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3721 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3781 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3841 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3901 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3961 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     4021 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     4081 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4141 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4201 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     4261 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     4321 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     4381 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     4441 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     4501 aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta
     4561 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     4621 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     4681 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     4741 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     4801 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     4861 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     4921 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     4981 attcgccatt caggctgcgc aactgttggg aagggcgatc g
//
