LOCUS       sequence-177644-        4696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expressio of human Syntenin2b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Koroll M, Rathjen FG, Volkmer H
  TITLE     The neural cell recognition molecule neurofascin interacts with 
            syntenin-1 but not with syntenin-2, both of which reveal 
            self-associating activity.
  JOURNAL   J Biol Chem. 2001 Apr 6;276(14):10646-54. doi: 
            10.1074/jbc.M010647200. Epub 2001 Jan 4.
  PUBMED    11152476
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M010647200"; journalName: "J Biol Chem"; date: 
            "2001-04-6- 6"; volume: "276"; issue: "14"; pages: "10646-54"
COMMENT     Sequence Label: pSG5-hSyntenin2b
FEATURES             Location/Qualifiers
     source          1..4696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..229
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        41..237
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      88..223
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          268..840
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     848..867
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     895..914
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        895..913
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1576..1697
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1613..1632)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1667..1686
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1731..1748)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1902..2490)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1982..2001)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2661..3452,3453..3521))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3284..3303
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3522..3626)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3694..3712
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3750..3772)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3872..3891
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(3958..4413)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4095..4116)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4307..4326
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4539..4561
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4553..4570
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4554..4570
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4607..4627)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
       61 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
      121 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
      181 tctgagctat tccagaagta gtgaagaggc ttttttggag gcctaggctt ttgcaaaaag
      241 ctccggatcg atcctgagaa cttcagggtg agtttgggga cccttgattg ttctttcttt
      301 ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt gttgtttaga
      361 atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt ctttcacttt
      421 ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt aactttttcg
      481 ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta tttgtcagat
      541 tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt atattgtact
      601 tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat atttctgcat
      661 ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacat cctggtcatc
      721 atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag gataaaatac
      781 tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctt tttcctacag
      841 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgtaatac
      901 gactcactat agggcgaatt ccagatggtg gcaccggtaa ccgggtacag cctgggcgtg
      961 cggcgagctg agatcaagcc cggggtgcgc gagatccacc tgtgcaagga cgagcgcggc
     1021 aagaccgggc tgaggctgcg gaaggtcgac caggggctct ttgtgcagtt ggtccaggcc
     1081 aacacccctg catcccttgt ggggctgcgc tttggggacc agctcctgca gattgacggg
     1141 cgtgactgtg ctgggtggag ctcgcacaaa gcccatcagg tggtgaagaa ggcatcaggc
     1201 gataagattg tcgtggtggt tcgggacagg ccgttccagc ggactgtcac catgcacaag
     1261 gacagcatgg gccacgtcgg cttcgtgatc aagaagggga agattgtctc tctggtcaaa
     1321 gggagttctg cggcctgcaa cgggctcctc accaaccact acgtgtgtga ggtggacggg
     1381 cagaatgtta tcgggctgaa ggacaaaaag atcatggaga ttctggccac ggctgggaac
     1441 gttgtcaccc tgaccatcat ccccagtgtg atctacgagc acatggtcaa aaagttgcct
     1501 ccagtcctgc tccaccacac catggaccac tccatcccag atgcctgaat tcggatccag
     1561 atcttattaa agcagaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     1621 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     1681 ctcatcaatg tatcttatca tgtctggtcg actctagact cttccgcttc ctcgctcact
     1741 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1801 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1861 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1921 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1981 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2041 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2101 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2161 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2221 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2281 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2341 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2401 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2461 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2521 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2581 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2641 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     2701 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     2761 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     2821 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     2881 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     2941 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3001 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3061 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3121 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3181 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3241 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3301 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3361 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3421 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3481 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3541 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3601 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     3661 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     3721 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     3781 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     3841 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     3901 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa
     3961 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     4021 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     4081 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     4141 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     4201 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     4261 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     4321 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     4381 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg
     4441 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     4501 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     4561 gacggccagt gaattgtcga cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     4621 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4681 aattagtcag caagga
//
