LOCUS       sequence-177646-        5925 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of rat Syntenin1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Koroll M, Rathjen FG, Volkmer H
  TITLE     The neural cell recognition molecule neurofascin interacts with 
            syntenin-1 but not with syntenin-2, both of which reveal 
            self-associating activity.
  JOURNAL   J Biol Chem. 2001 Apr 6;276(14):10646-54. doi: 
            10.1074/jbc.M010647200. Epub 2001 Jan 4.
  PUBMED    11152476
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M010647200"; journalName: "J Biol Chem"; date: 
            "2001-04-6- 6"; volume: "276"; issue: "14"; pages: "10646-54"
COMMENT     Sequence Label: pCMV-Myc-rSyntenin1
FEATURES             Location/Qualifiers
     source          1..5925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     11..33
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     25..42
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     26..42
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        69..372
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        373..576
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     527..547
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     573..597
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          714..810
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    3106..3240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3130..3149)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3184..3203
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3351..3367)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3351..3367)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3364..3386)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3375..3391
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3399..3405,3406..3423,3424..3429))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3444..3465
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3582..3599)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3753..4341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3833..3852)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4512..5303,5304..5372))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5135..5154
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5373..5477)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5545..5563
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5601..5623)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5723..5742
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagttcga gcttgcatgc
       61 ctgcaggtcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      121 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      181 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      241 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      301 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      361 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      421 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      481 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      541 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      601 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
      661 cggactctag aggatccggt actagaggaa ctgaaaaacc agaaagttaa ctggtaagtt
      721 tagtcttttt gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg
      781 ctcctcagtg gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa
      841 aagctgcgga attgtacccg cgggcccacc atggcatcaa tgcagaagct gatctcagag
      901 gaggacctgc ttatggccat ggaggcccga attcggtcga ccgagatctc tcgaggatcc
      961 gaattcgcgg ccgcgtcgac agcggcagga acatcgcggt gttcggggca gaaaacacct
     1021 gcaaaaatgt ctctctatcc atctcttgaa gatttgaagg tagacaaagt aattcaggct
     1081 caaactgcct cttctgctaa tcctgccagc caggcctttt ttttaaatgt ggtcgattct
     1141 gctgcctttc ctccaggtgg aaatctctat cctaaactgt atccagaact ctctcaatac
     1201 atgggactgt ctttaagtga agctgaaata tgtgaaaaca tgcctgtggt ctctggagcg
     1261 ccaacccaag ggcagttggt agcaaggcca tccagtgtga actacatggt agcacctgtg
     1321 acaggtaacg atgcggggat taggagagca gagattaagc aagggattcg ggaagtcatt
     1381 ttgtgtaagg atcaagatgg aaaaattggg ctcagactga agtcagtaga taatggtata
     1441 tttgttcaac tagtccaggc taattcccca gcctccttgg ttgggctgcg atttggggac
     1501 caggtgctcc agatcaatgg tgaaaactgt gccggctgga gctccgataa ggcacacaag
     1561 gtgctcaagc aggcctttgg agagaagatt accatgacca ttcgtgatag accctttgaa
     1621 cggacagtta ccatgcataa ggacagcagt ggacatgttg gctttatctt taaaagtgga
     1681 aaaatcacat ccatagtgaa agacagttct gctgccagaa acggccttct cactgatcac
     1741 cacatctgtg agatcaacgg acagaacgtc attggcttga aggatgctca gattgcagat
     1801 atactgtcaa cagctgggac tgtggtcacc atcacgatca tgcctgcctt tatcttcgaa
     1861 catattatta aacggatggc tcccagcatt atgaaaagcc tgatggatca caccattcct
     1921 gaagtttaaa ccttgtacag tgcatcaccg ccgaacgctt ccagtcttcc tcttcggcaa
     1981 cttctctatt tcaccgcaca tgaagctttc ccggatccag ggagactgtg ctgcatggag
     2041 acctgtctat cttccaccat ggctgggaac ctcactctat ctgagagcgt cttcagattt
     2101 taagataggt gtagccttat cctggtttta cagatgtgaa actctcaaaa gattccctga
     2161 ctttctagac ttggttcttt actgggaacc tgatgttttt ctataagcca ttgtgattag
     2221 gatgactggt gtaggcttag ctgtgtccga gaactggtct gctttctccg agaagtgcac
     2281 agtgctaggc acgtgcttca gttctgtggc atatgtggat tgtagtacat cagcatagta
     2341 cctgtaggat gattgccttt gatcgctact ttttaagttt gtgtgtgagt gtgtaaaatt
     2401 ccagttaatc aacacttcca ttccacaaaa gcattataca gttctgtgta ggtggcaaga
     2461 gactaatgat tgtcactaaa ttctaactac cttcacttaa tgtgcttcag ttgtcgcctt
     2521 aactacagta agcatttaga ataaaagcca aaatagaatt attgctgcct tctgaaacct
     2581 gagaggttag ctctctatca agctgttcta tgctagccct gagcagctta ctggtgtcgc
     2641 agagctgcct agctttacca agattttcta gtcactgcat aaaggaaacg cctaagagtt
     2701 gccgtattgc tttctgagat acaaattgct atatatttca tgtaaaagtt gttatactaa
     2761 ataggataaa ttttggagtc tctttttcac tcgtagatta agtgggtaat atctaatttg
     2821 gcacttgagt cctaacatta tgtaacttta gctaggttgg ggtgggttta gaatgtttcc
     2881 ctagtcatct cagtttgttc cattttctga ctctatcctg caaacaaaaa tggcaatgac
     2941 actttatcct gactctttcc ttcttagttt gtgcctatct tattatttta tgtgatatta
     3001 tcctattaaa tgtataaaaa tattaaatgt caaaaaaaaa aaaaaaaaaa aaaaaagtcg
     3061 acgcggccgc gaattccaga tctctcgagg taccgcggcc gcggggatcc agacatgata
     3121 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     3181 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     3241 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     3301 tcggatcctc tagagtcgat ctgcaggcat gctagcttgg cgtaatcatg gtcatagctg
     3361 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     3421 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     3481 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     3541 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     3601 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     3661 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     3721 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     3781 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     3841 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3901 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     3961 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4021 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4081 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4141 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     4201 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4261 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     4321 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     4381 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     4441 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     4501 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4561 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4621 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     4681 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     4741 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     4801 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     4861 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     4921 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     4981 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5041 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5101 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5161 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5221 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5281 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5341 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     5401 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5461 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     5521 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     5581 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     5641 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     5701 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     5761 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     5821 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     5881 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacg
//
