LOCUS       sequence-177663-        8777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human codon optimized RecA protein was fused to SpCas9 for enhanced 
            genome editing efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Lin L, Petersen TS, Jensen KT, Bolund L, Kuhn R, Luo Y
  TITLE     Fusion of SpCas9 to E. coli Rec A protein enhances CRISPR-Cas9 
            mediated gene knockout in mammalian cells.
  JOURNAL   J Biotechnol. 2017 Mar 1;247:42-49. doi: 
            10.1016/j.jbiotec.2017.02.024.
  PUBMED    28259533
REFERENCE   2  (bases 1 to 8777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2017-03-1"; volume: "247:42-49. doi"; pages: " 
            10.1016/j.jbiotec.2017.02.024"
COMMENT     Sequence Label: P628_SpCas9-RecA
FEATURES             Location/Qualifiers
     source          1..8777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             121..4221
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             121..4221
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4222..4269
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5353..5373
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5404..5421)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5410..5617
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5626..5766
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5626..5755
                     /label=AAV2 ITR
     rep_origin      5841..6296
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5928..5947)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6138..6159
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6313..6332)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6432..6454
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6492..6510)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6578..6682
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6683..6751,6752..7543)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6901..6920)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7714..8302
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8203..8222
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8364..8612
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8364..8384
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8535..8554
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8631..8706
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 atggactata aggaccacga cggagactac aaggatcatg atattgatta caaagacgat
       61 gacgataaga tggccccaaa gaagaagcgg aaggtcggta tccacggagt cccagcagcc
      121 gacaagaagt acagcatcgg cctggacatc ggcaccaact ctgtgggctg ggccgtgatc
      181 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
      241 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
      301 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
      361 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
      421 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
      481 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
      541 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
      601 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
      661 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
      721 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg
      781 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg
      841 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
      901 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
      961 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
     1021 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
     1081 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
     1141 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
     1201 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
     1261 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag
     1321 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
     1381 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
     1441 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
     1501 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg
     1561 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac
     1621 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat
     1681 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc
     1741 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
     1801 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
     1861 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
     1921 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
     1981 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
     2041 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
     2101 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
     2161 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc
     2221 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
     2281 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
     2341 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
     2401 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
     2461 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
     2521 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
     2581 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc
     2641 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac
     2701 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
     2761 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
     2821 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
     2881 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
     2941 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
     3001 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
     3061 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac
     3121 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
     3181 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
     3241 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct
     3301 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
     3361 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
     3421 acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc
     3481 agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat
     3541 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
     3601 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
     3661 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
     3721 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag
     3781 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
     3841 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
     3901 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
     3961 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
     4021 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
     4081 gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag
     4141 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
     4201 ctgtctcagc tgggaggcga caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa
     4261 aagaaaaagg aattcggcgg aggcggcagc atggccatcg acgagaacaa gcagaaggcc
     4321 ctggccgctg ccctgggcca gatcgagaag cagtttggca agggcagcat catgcggctg
     4381 ggcgaggaca gatccatgga cgtggaaacc atcagcaccg gcagcctgag cctggatatc
     4441 gctctgggag ctggcggcct gcctatgggc agaatcgtgg aaatctacgg ccccgagagc
     4501 agcggcaaga ccaccctgac actgcaagtg atcgctgccg cccagcggga aggcaagacc
     4561 tgcgccttta tcgatgccga gcacgccctg gaccccatct acgccagaaa gctgggagtg
     4621 gacatcgaca acctgctgtg cagccagccc gatacaggcg aacaggccct ggaaatctgc
     4681 gacgccctgg ctagatctgg cgccgtggat gtgatcgtgg tggattctgt ggccgccctg
     4741 acccccaagg ccgagattga gggcgagatc ggcgatagcc acatgggcct ggccgccaga
     4801 atgatgagcc aggccatgag gaagctggcc ggcaacctga agcagagcaa caccctgctg
     4861 atcttcatca accagatccg catgaagatc ggcgtgatgt tcggcaaccc cgagacaacc
     4921 acaggcggca acgccctgaa gttctacgcc tccgtgcggc tggacatcag acggatcgga
     4981 gccgtgaaag agggggagaa cgtcgtgggc agcgagacaa gagtgaaggt cgtgaagaac
     5041 aagatcgccg ctcccttcaa gcaggccgag ttccagatcc tgtacggcga gggcatcaac
     5101 ttctacggcg agctggtgga cctgggagtg aaagagaagc tgattgagaa ggctggcgcc
     5161 tggtacagct acaagggcga gaagatcgga cagggcaagg ccaatgccac cgcctggctg
     5221 aaggacaacc ccgaaaccgc caaagaaatc gagaagaaag tgcgcgagct gctgctgagc
     5281 aaccccaaca gcacccccga cttcagcgtg gacgattctg agggcgtggc cgagacaaac
     5341 gaggacttcc cacccaagaa aaagcggaaa gtctgagaat tctaactaga gctcgctgat
     5401 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     5461 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     5521 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     5581 gggaggattg ggaagagaat agcaggcatg ctggggagcg gccgcaggaa cccctagtga
     5641 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     5701 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     5761 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     5821 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     5881 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
     5941 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     6001 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     6061 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     6121 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
     6181 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
     6241 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     6301 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     6361 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     6421 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     6481 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     6541 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     6601 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6661 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6721 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     6781 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     6841 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     6901 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     6961 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     7021 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     7081 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     7141 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     7201 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     7261 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7321 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     7381 tggagccggt gagcgtggaa gccgcggtat cattgcagca ctggggccag atggtaagcc
     7441 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     7501 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     7561 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7621 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7681 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7741 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7801 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7861 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7921 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7981 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     8041 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     8101 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     8161 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     8221 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8281 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8341 ttgctggcct tttgctcaca tgtgagggcc tatttcccat gattccttca tatttgcata
     8401 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
     8461 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
     8521 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
     8581 ctttatatat cttgtggaaa ggacgaaaca ccgggtcttc gagaagacct gttttagagc
     8641 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     8701 cggtgctttt ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttttta
     8761 gcgcgtgcgc caattct
//
