LOCUS       sequence-177671-       13498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  jStack yeast assembly into plant vectors, Level 2 pYB Vector, Hygro 
            selection .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13498)
  AUTHORS   Shih PM, Vuu K, Mansoori N, Ayad L, Louie KB, Bowen BP, Northen TR, 
            Loque D
  TITLE     A robust gene-stacking method utilizing yeast assembly for plant 
            synthetic biology.
  JOURNAL   Nat Commun. 2016 Oct 26;7:13215. doi: 10.1038/ncomms13215.
  PUBMED    27782150
REFERENCE   2  (bases 1 to 13498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-10-26"; volume: "7:13215. doi"; pages: " 
            10.1038/ncomms13215"
COMMENT     Sequence Label: Pyb1301(JBEI-11629)
FEATURES             Location/Qualifiers
     source          1..13498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    496..517
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(565..1272)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        1284..1499
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             1500..2303
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      2385..2637
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2687..2711
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4012..4641
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5070..6143
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6209..6403
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6639..6661)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6747..6887
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6802..6821
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6902..6919)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7073..7661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7153..7172)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7917..8324
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             8325..9419
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(10499..11293)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11718..11742
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11820..11994
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(12035..13060)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(join(13127..13498,1..305))
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(13170..13190)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc
       61 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
      121 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
      181 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
      241 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
      301 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
      361 tgtcgtgctc caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc
      421 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
      481 tgagcgcaac gcaattaatg tgagttagct cactcattag gcatctagta cgcgtcctgc
      541 aggcgcgccg aggggatcga gcccctgctg agcctcgaca tgttgtcgca aaattcgccc
      601 tggacccgcc caacgatttg tcgtcactgt caaggtttga cctgcacttc atttggggcc
      661 cacatacacc aaaaaaatgc tgcataattc tcggggcagc aagtcggtta cccggccgcc
      721 gtgctggacc gggttgaatg gtgcccgtaa ctttcggtag agcggacggc caatactcaa
      781 cttcaaggaa tctcacccat gcgcgccggc ggggaaccgg agttcccttc agtgagcgtt
      841 attagttcgc cgctcggtgt gtcgtagata ctagcccctg gggcactttt gaaatttgaa
      901 taagatttat gtaatcagtc ttttaggttt gaccggttct gccgcttttt ttaaaattgg
      961 atttgtaata ataaaacgca attgtttgtt attgtggcgc tctatcatag atgtcgctat
     1021 aaacctattc agcacaatat attgttttca ttttaatatt gtacatataa gtagtagggt
     1081 acaatcagta aattgaacgg agaatattat tcataaaaat acgatagtaa cgggtgatat
     1141 attcattaga atgaaccgaa accggcggta aggatctgag ctacacatgc tcaggttttt
     1201 tacaacgtgc acaacagaat tgaaagcaaa tatcatgcga tcataggcgt ctcgcatatc
     1261 tcattaaagc agcacgtgag cttttcaatt catcattttt tttttattct tttttttgat
     1321 ttcggtttcc ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga
     1381 aggagcacag acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt
     1441 gcccagtatt cttaacccaa ctgcacagaa caaaaacatg caggaaacga agataaatca
     1501 tgtcgaaagc tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc
     1561 tatttaatat catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca
     1621 ccaaggaatt actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac
     1681 atgtggatat cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat
     1741 ccgccaagta caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag
     1801 tcaaattgca gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg
     1861 cacacggtgt ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa
     1921 caaaggaacc tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta
     1981 ctggagaata tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg
     2041 gctttattgc tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga
     2101 cacccggtgt gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg
     2161 atgatgtggt ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg
     2221 gaagggatgc taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga
     2281 gaagatgcgg ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact
     2341 cacaaattag agcttcaatt taattatatc agttattacc ccacgtgcgt tcaaacattt
     2401 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
     2461 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
     2521 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
     2581 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg
     2641 cctgcaggac gcgtggcgcg ccagatccaa ttaaactatc agtgtttgac aggatatatt
     2701 ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt
     2761 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg
     2821 atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag
     2881 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg
     2941 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt
     3001 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct
     3061 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg
     3121 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc
     3181 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc
     3241 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg
     3301 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg
     3361 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc
     3421 ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca
     3481 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag
     3541 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg
     3601 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg
     3661 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca
     3721 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc
     3781 ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct
     3841 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac
     3901 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
     3961 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
     4021 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
     4081 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
     4141 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
     4201 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
     4261 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
     4321 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
     4381 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
     4441 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
     4501 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
     4561 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
     4621 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
     4681 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
     4741 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
     4801 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
     4861 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
     4921 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
     4981 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg cctgccggcc ctgcaatggc
     5041 actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac
     5101 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
     5161 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
     5221 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
     5281 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
     5341 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
     5401 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
     5461 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
     5521 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
     5581 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
     5641 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
     5701 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
     5761 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
     5821 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
     5881 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
     5941 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
     6001 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
     6061 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
     6121 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
     6181 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc
     6241 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
     6301 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
     6361 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
     6421 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
     6481 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca
     6541 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag
     6601 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     6661 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     6721 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     6781 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     6841 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     6901 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6961 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7021 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7081 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7141 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7201 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7261 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7321 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7381 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7441 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7501 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7561 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7621 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaacaattc cgaccgtcga
     7681 ggagaacttc tagtatatct acatacctaa tattattgcc ttattaaaaa tggaatccca
     7741 acaattacat caaaatccac attctcttca aaatcaattg tcctgtactt ccttgttcat
     7801 gtgtgttcaa aaacgttata tttataggat aattatactc tatttctcaa caagtaattg
     7861 gttgtttggc cgagcggtct aaggcgcctg attcaagaaa tatcttgacc gcagttaact
     7921 gtgggaatac tcaggtatcg taagatgcaa gagttcgaat ctcttagcaa ccattatttt
     7981 tttcctcaac ataacgagaa cacacagggg cgctatcgca cagaatcaaa ttcgatgact
     8041 ggaaattttt tgttaatttc agaggtcgcc tgacgcatat acctttttca actgaaaaat
     8101 tgggagaaaa aggaaaggtg agagcgccgg aaccggcttt tcatatagaa tagagaagcg
     8161 ttcatgacta aatgcttgca tcacaatact tgaagttgac aatattattt aaggacctat
     8221 tgttttttcc aataggtggt tagcaatcgt cttactttct aacttttctt accttttaca
     8281 tttcagcaat atatatatat atatttcaag gatataccat tctaatgtct gcccctaaga
     8341 agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat cacagccgaa gccattaagg
     8401 ttcttaaagc tatttctgat gttcgttcca atgtcaagtt cgatttcgaa aatcatttaa
     8461 ttggtggtgc tgctatcgat gctacaggtg ttccacttcc agatgaggcg ctggaagcct
     8521 ccaagaaggc tgatgccgtt ttgttaggtg ctgtgggtgg tcctaaatgg ggaaccggta
     8581 gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga acttcaattg tacgccaact
     8641 taagaccatg taactttgca tccgactctc ttttagactt atctccaatc aagccacaat
     8701 ttgctaaagg tactgacttc gttgttgtca gagaattagt gggaggtatt tactttggta
     8761 agagaaagga agacgatggt gatggtgtcg cttgggatag tgaacaatac accgttccag
     8821 aagtgcaaag aatcacaaga atggccgctt tcatggccct acaacatgag ccaccattgc
     8881 ctatttggtc cttggataaa gctaatgttt tggcctcttc aagattatgg agaaaaactg
     8941 tggaggaaac catcaagaac gaatttccta cattgaaggt tcaacatcaa ttgattgatt
     9001 ctgccgccat gatcctagtt aagaacccaa cccacctaaa tggtattata atcaccagca
     9061 acatgtttgg tgatatcatc tccgatgaag cctccgttat cccaggttcc ttgggtttgt
     9121 tgccatctgc gtccttggcc tctttgccag acaagaacac cgcatttggt ttgtacgaac
     9181 catgccacgg ttctgctcca gatttgccaa agaataaggt caaccctatc gccactatct
     9241 tgtctgctgc aatgatgttg aaattgtcat tgaacttgcc tgaagaaggt aaggccattg
     9301 aagatgcagt taaaaaggtt ttggatgcag gtatcagaac tggtgattta ggtggttcca
     9361 acagtaccac cgaagtcggt gatgctgtcg ccgaagaagt taagaaaatc cttgcttaaa
     9421 aagattctct ttttttatga tatttgtaca taaactttat aaatgaaatt cataatagaa
     9481 acgacacgaa attacaaaat ggaatatgtt catagggtag acgaaactat atacgcaatc
     9541 tacatacatt tatcaagaag gagaaaaagg aggatgtaaa ggaatacagg taagcaaatt
     9601 gatactaatg gctcaacgtg ataaggaaaa agaattgcac tttaacatta atattgacaa
     9661 ggaggagggc accacacaaa aagttaggtg taacagaaaa tcatgaaact atgattccta
     9721 atttatatat tggaggattt tctctaaaaa aaaaaaaata caacaaataa aaaacactca
     9781 atgacctgac catttgatgg agtttaagtc aataccttct tgaaccattt cccataatgg
     9841 tgaaagttcc ctcaagaatt ttactctgtc agaaacggcc ttaacgacgt agtcgatgac
     9901 ggatcgcttg cctgtaactt acacgcgcct cgtatctttt aatgatggaa taatttggga
     9961 atttactctg tgtttattta tttttatgtt ttgtatttgg attttagaaa gtaaataaag
    10021 aaggtagaag agttacggaa tgaagaaaaa aaaataaaca aaggtttaaa aaatttcaac
    10081 aaaaagcgta ctttacatat atatttatta gacaagaaaa gcagattaaa tagatataca
    10141 ttcgattaac gataagtaaa atgtaaaatc acaggatttt cgtgtgtggt cttctacaca
    10201 gacaagatga aacaattcgg cattaatacc tgagagcagg aagagcaaga taaaaggtag
    10261 tatttgttgg cgatccccct agagtctttt acatcttcgg aaaacaaaaa ctattttttc
    10321 tttaatttct ttttttactt tctattttta atttatatat ttatattaaa aaatttaaat
    10381 tataattatt tttatagcac gtcatctgcg agctcgatcc tttgatcttt tctacggggt
    10441 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact
    10501 aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca
    10561 gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga
    10621 cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc
    10681 cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga
    10741 caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa
    10801 atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt
    10861 aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga
    10921 tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt
    10981 catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc
    11041 agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata
    11101 gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca
    11161 tttttaaata taggttttca ttttctccca ccagcttata taccttagca ggagacattc
    11221 cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc
    11281 tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga
    11341 agctaattat aacaagacga actccaattc actgttcctt gcattctaaa accttaaata
    11401 ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga
    11461 gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca
    11521 tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg
    11581 aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg
    11641 tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
    11701 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
    11761 cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt aattcggggg
    11821 atctggattt tagtactgga ttttggtttt aggaattaga aattttattg atagaagtat
    11881 tttacaaata caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct
    11941 ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga gaaactcgag
    12001 cttgtcgatc gacagatccc ggtcggcatc tactctattt ctttgccctc ggacgagtgc
    12061 tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg
    12121 cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt
    12181 cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga
    12241 gttggtcaag accaatgcgg agcatatacg cccggagtcg tggcgatcct gcaagctccg
    12301 gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga
    12361 agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga
    12421 ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga
    12481 ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga
    12541 cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa
    12601 tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc
    12661 cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatagcc tccgcgaccg
    12721 gttgtagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgaac
    12781 ggcgggagat gcaataggtc aggctctcgc taaactcccc aatgtcaagc acttccggaa
    12841 tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg
    12901 cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag
    12961 attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa
    13021 acttctcgac agacgtcgcg gtgagttcag gctttttcat atctcattgc cccccggatc
    13081 tgcgaaagct cgagagagat agatttgtag agagagactg gtgatttcag cgtgtcctct
    13141 ccaaatgaaa tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg
    13201 cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg
    13261 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
    13321 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
    13381 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
    13441 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctga
//
