LOCUS       sequence-177682-        4571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Expresses AMPK alpha 1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: pECE HA AMPKa1 AS
FEATURES             Location/Qualifiers
     source          1..4571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    115..249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(152..171)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     206..225
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(308..327)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(592..609)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(763..1351)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(843..862)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1522..2313,2314..2382))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2145..2164
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2383..2487)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2542..2562)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             2877..2903
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 taagtaatga tcataatcag ccatatcaca tctgtagagg ttttacttgc tttaaaaaac
       61 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      121 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
      181 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
      241 gtctggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
      301 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
      361 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
      421 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
      481 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgatatgc ggtgtgaaat
      541 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
      601 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      661 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      721 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      781 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      841 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      901 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      961 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1021 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1081 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1141 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1201 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1261 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1321 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1381 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1441 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1501 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1561 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     1621 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     1681 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1741 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1801 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
     1861 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1921 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1981 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     2041 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     2101 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     2161 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     2221 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     2281 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     2341 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     2401 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2461 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaccttct
     2521 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     2581 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaagg aaagtcccca
     2641 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     2701 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     2761 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     2821 ttccagaagt agtgaagagg cttttttgga ggagatctaa gcttgccgcc accatgtatg
     2881 atgttcctga ttatgctagc ctcccgggga attcggcgac agccgagaag cagaaacacg
     2941 acgggcgggt gaagatcggc cactacattc tgggtgacac gctgggggtc ggcaccttcg
     3001 gcaaagtgaa ggttggcaaa catgaattga ctgggcataa agtagctgtg aagatactca
     3061 atcgacagaa gattcggagc cttgatgtgg taggaaaaat ccgcagagaa attcagaacc
     3121 tcaagctttt caggcatcct catataatta aactgtacca ggtcatcagt acaccatctg
     3181 atattttcat ggtgggggaa tatgtctcag gaggagagct atttgattat atctgtaaga
     3241 atggaaggct ggatgaaaaa gaaagtcggc gtctgttcca acagatcctt tctggtgtgg
     3301 attattgtca caggcatatg gtggtccata gagatttgaa acctgaaaat gtcctgcttg
     3361 atgcacacat gaatgcaaag atagctgatt ttggtctttc aaacatgatg tcagatggtg
     3421 aatttttaag aacaagttgt ggctcaccca actatgctgc accagaagta atttcaggaa
     3481 gattgtatgc aggcccagag gtagatatat ggagcagtgg ggttattctc tatgctttat
     3541 tatgtggaac ccttccattt gatgatgacc atgtgccaac tctttttaag aagatatgtg
     3601 atgggatctt ctatacccct caatatttaa atccttctgt gattagcctt ttgaaacata
     3661 tgctgcaggt ggatcccatg aagagggcca caatcaaaga tatcagggaa catgaatggt
     3721 ttaaacagga ccttccaaaa tatctctttc ctgaggatcc atcatatagt tcaaccatga
     3781 ttgatgatga agccttaaaa gaagtatgtg aaaagtttga gtgctcagaa gaggaagttc
     3841 tcagctgtct ttacaacaga aatcaccagg atcctttggc agttgcctac catctcataa
     3901 tagataacag gagaataatg aatgaagcca aagatttcta tttggcgaca agcccacctg
     3961 attcttttct tgatgatcat cacctgactc ggccccatcc tgaaagagta ccattcttgg
     4021 ttgctgaaac accaagggca cgccataccc ttgatgaatt aaatccacag aaatccaaac
     4081 accaaggtgt aaggaaagca aaatggcatt taggaattag aagtcaaagt cgaccaaatg
     4141 atattatggc agaagtatgt agagcaatca aacaattgga ttatgaatgg aaggttgtaa
     4201 acccatatta tttgcgtgta cgaaggaaga atcctgtgac aagcacttac tccaaaatga
     4261 gtctacagtt ataccaagtg gatagtagaa cttatctact ggatttccgt agtattgatg
     4321 atgaaattac agaagccaaa tcagggactg ctactccaca gagatcggga tcagttagca
     4381 actatcgatc ttgccaaagg agtgattcag atgctgaggc tcaaggaaaa tcctcagaag
     4441 tttctcttac ctcatctgtg acctcacttg actcttctcc tgttgaccta actccaagac
     4501 ctggaagtca cacaatagaa ttttttgaga tgtgtgcaaa tctaattaaa attcttgcac
     4561 aataatctag a
//
