LOCUS       sequence-177683-        9208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the N1041 truncation to mouse TET2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Nakagawa T, Lv L, Nakagawa M, Yu Y, Yu C, D'Alessio AC, Nakayama K, 
            Fan HY, Chen X, Xiong Y
  TITLE     CRL4(VprBP) E3 Ligase Promotes Monoubiquitylation and Chromatin 
            Binding of TET Dioxygenases.
  JOURNAL   Mol Cell. 2015 Jan 22;57(2):247-60. doi: 
            10.1016/j.molcel.2014.12.002. Epub 2014 Dec 31.
  PUBMED    25557551
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.12.002"; journalName: "Mol Cell"; date: 
            "2015-01-22- 22"; volume: "57"; issue: "2"; pages: "247-60"
COMMENT     Sequence Label: pcDNA3-FLAG-mTET2 (N1041)
FEATURES             Location/Qualifiers
     source          1..9208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      175..310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     237..256
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             391..1185
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(445..464)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1055..1074
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1359..1480
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1396..1415)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1450..1469
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1529..1545)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1529..1545)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1542..1564)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1553..1569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1577..1583,1584..1601,1602..1607))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1622..1643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1760..1777)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1931..2519)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2011..2030)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2690..3481,3482..3550))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3313..3332
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3551..3655)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3730..3749)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3921..4300
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4301..4504
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4455..4475
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4549..4568
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4549..4567
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          4604..5176
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             5222..5245
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(8447..8465)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8448..8465)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8485..8502)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8491..8715
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8761..9189
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8848..8867)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9058..9079
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(join(9198..9208,1..10))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(9203..9208,1..324)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
       61 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      121 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
      181 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
      241 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
      301 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
      361 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
      421 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
      481 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
      541 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
      601 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
      661 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
      721 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
      781 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
      841 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
      901 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
      961 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     1021 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     1081 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     1141 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     1201 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     1261 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     1321 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     1381 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     1441 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     1501 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     1561 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     1621 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     1681 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1741 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1801 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1861 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1921 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1981 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2041 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2101 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2161 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2221 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2281 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2341 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2401 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2461 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2521 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2581 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2641 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2701 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2761 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2821 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2881 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2941 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3001 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3061 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3121 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3181 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3241 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3301 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3361 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3421 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3481 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3541 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3601 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3661 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     3721 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     3781 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     3841 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     3901 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
     3961 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     4021 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     4081 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     4141 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     4201 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     4261 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     4321 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     4381 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     4441 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     4501 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
     4561 ctatagggag acccaagcta gagtcgatcc tgagaacttc agggtgagtt tggggaccct
     4621 tgattgttct ttctttttcg ctattgtaaa attcatgtta tatggagggg gcaaagtttt
     4681 cagggtgttg tttagaatgg gaagatgtcc cttgtatcac catggaccct catgataatt
     4741 ttgtttcttt cactttctac tctgttgaca accattgtct cctcttattt tcttttcatt
     4801 ttctgtaact ttttcgttaa actttagctt gcatttgtaa cgaattttta aattcacttt
     4861 tgtttatttg tcagattgta agtactttct ctaatcactt ttttttcaag gcaatcaggg
     4921 tatattatat tgtacttcag cacagtttta gagaacaatt gttataatta aatgataagg
     4981 tagaatattt ctgcatataa attctggctg gcgtggaaat attcttattg gtagaaacaa
     5041 ctacatcctg gtcatcatcc tgcctttctc tttatggtta caatgatata cactgtttga
     5101 gatgaggata aaatactctg agtccaaacc gggcccctct gctaaccatg ttcatgcctt
     5161 cttctttttc ctacagctcc tgggcaacgt gctggtttgt gctgtcgacc ccaagcttat
     5221 ggactacaaa gacgatgacg acaagggatc cactagtaac ggccgccagt gtgctggaat
     5281 tcgcccttta gaacaggaca gaaccaccca tgctgagggc accagactga gtccattcct
     5341 gatagcacca ccttctccca tcagccatac agaacctctg gctgtcaaac tccagaatgg
     5401 aagcccgtta gcagagagac ctcatccaga agtaaatgga gacaccaagt ggcaatcttc
     5461 ccaaagctgt tatggaatat cccacatgaa aggcagccag agcagtcatg agagtccaca
     5521 tgaagacaga gggtattcca ggtgcttaca gaatggaggg ataaaacgca cagtcagtga
     5581 accatctctc tctgggctcc atccaaacaa gatattgaaa ctagaccaaa aagctaaggg
     5641 agaaagcaat atcttcgagg aaagccaaga aagaaaccac ggtaaaagca gccgtcagcc
     5701 aaatgtctcc ggactaagtg ataatggaga gcccgtgacc tcgaccaccc aggaaagttc
     5761 aggtgcagat gctttcccaa cacggaacta caatggagtt gaaattcagg ttctcaacga
     5821 gcaggaaggg gaaaaaggca ggagcgttac attacttaaa aacaagattg tgctaatgcc
     5881 taatggtgct acagtttctg cccattctga ggagaacaca cgtggtgaac tcctggaaaa
     5941 aacacagtgt tatccagatt gtgtctccat tgccgtgcag agtaccgcat ctcatgtaaa
     6001 cacccctagc agtcaggctg ctatcgagtt gtcccacgag atccctcaac catcgcttac
     6061 ctcagcgcag atcaatttct cccagacctc aagcttgcag ctgcctccag agccagctgc
     6121 aatggtgact aaggcctgtg atgctgataa tgccagtaaa ccagctatag taccaggtac
     6181 ctgtcctttt cagaaagcag aacaccaaca aaagtcagct ttggacatag gcccatctcg
     6241 tgcagaaaac aaaaccatcc aaggaagcat ggagctattt gctgaagaat actatcctag
     6301 ttccgaccgg aatttgcaag cttcgcatgg cagctctgaa cagtattcaa agcaaaagga
     6361 aaccaatggt gcttacttca ggcaaagctc gaagttccct aaagattcca tctctcccac
     6421 tactgtgacc ccaccgtcac aatcacttct tgctccccgt cttgttcttc agcctccttt
     6481 agaaggaaaa ggcgctctaa atgatgtagc tttggaagaa caccatgact accccaaccg
     6541 aagcaaccga actcttttaa gggaagggaa aatagaccat caacccaaga catcatctag
     6601 ccagagtctg aatccatctg tacatacacc caaccccccc ttgatgcttc cagaacagca
     6661 tcagaatgat tgtggctcac cgagccctga aaagtcaaga aaaatgtcag aatatctcat
     6721 gtattacctg ccaaatcatg gccacagtgg aggtttacaa gaacatagcc aatacctgat
     6781 ggggcacagg gagcaagaga ttccgaagga tgcaaacggg aaacaaacgc aaggctctgt
     6841 acaggcagca cctggctgga tagaactgaa agccccgaat ttgcatgaag cactccatca
     6901 gacaaaacgc aaggatatat ccttgcactc agtcctccac tctcagaccg gccctgtcaa
     6961 tcagatgagc tccaaacagt ccactggcaa tgtcaacatg ccaggaggat tccaaaggct
     7021 accttacctc cagaaaacag cccagccaga gcagaaggca caaatgtacc aagtgcaagt
     7081 gaaccaagga ccgtctccag gtatggggga ccaacatctt cagttccaga aagctttata
     7141 ccaggagtgc atccccagga cagatccgtc atctgaggct cacccgcaag caccgagcgt
     7201 tcctcagtat catttccagc aaagagtaaa tccctccagt gataagcatt tgagtcaaca
     7261 ggccacagag actcaacggt tatcaggctt tttacaacat actcctcaga cgcaggcatc
     7321 acaaacacca gcatcccaga actcaaattt ccctcaaatc tgccagcagc agcagcagca
     7381 gcagcagcag ttacagagga agaataaaga gcaaatgcct cagactttct ctcatctcca
     7441 aggtagcaat gataagcaaa gagaaggctc gtgctttggc cagattaaag tggaagaaag
     7501 cttttgtgtc ggaaatcagt actccaaatc aagtaatttc caaactcaca ataataccca
     7561 aggggggttg gagcaagtac aaaatataaa taaaaatttt ccttattcga agatcttaac
     7621 accaaattcg agcaacttac agattctccc ttcaaatgac acacacccgg cttgtgagcg
     7681 ggaacaagct ctacatcccg taggaagtaa gacctcaaac ctgcagaaca tgcagtattt
     7741 cccgaataat gtgaccccaa atcaggacgt tcaccggtgc tttcaggaac aagcgcagaa
     7801 gcctcagcaa gcttcgtctc tacaggggct taaggacaga agccagggtg agtctccagc
     7861 cccaccagct gaggcagctc aacagaggta tttggtgcat aatgaagcaa aggcactccc
     7921 tgtgcctgag caaggaggaa gtcagacaca gacccctcct cagaaggaca ctcagaagca
     7981 cgctgcctta aggtggcttc tcttacagaa gcaagaacag cagcaaacac agcaatccca
     8041 gcctggtcat aaccagatgc ttaggccaat caagactgag cctgtatcca aaccttcttc
     8101 ctatagatac cccttgtcac cgccacaaga aaatatgtcc agcaggataa agcaagagat
     8161 ctcctctcca agccgtgaca atgggcagcc aaagagcatc attgagacca tggaacagca
     8221 cctgaagcag tttcagctca agtcactctg tgactataag gctctgactc tcaagtcaca
     8281 gaaacacgtg aaagtgccaa cagatatcca ggctgcagaa tcggagaacc acgcccgagc
     8341 tgcagagcct caagcaacca aaagcacaga ttgttctgtt ctcgacgatg tttcagaatc
     8401 agatactcct ggtgaatgac tcgagcatgc atctagaggg ccctattcta tagtgtcacc
     8461 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     8521 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     8581 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     8641 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     8701 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     8761 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     8821 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8881 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8941 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     9001 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     9061 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     9121 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     9181 acgcgaatta attctgtgga atgtgtgt
//
