LOCUS       sequence-177699-       11322 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, 
            H840A ) protein with a human AID mutant protein (p182X). MSCV 
            Retroviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11322)
  AUTHORS   Ma Y, Zhang J, Yin W, Zhang Z, Song Y, Chang X
  TITLE     Targeted AID-mediated mutagenesis (TAM) enables efficient genomic 
            diversification in mammalian cells.
  JOURNAL   Nat Methods. 2016 Dec;13(12):1029-1035. doi: 10.1038/nmeth.4027. 
            Epub 2016 Oct 10.
  PUBMED    27723754
REFERENCE   2  (bases 1 to 11322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4027"; journalName: "Nat Methods"; date: "2016-12"; 
            volume: "13"; issue: "12"; pages: "1029-1035"
COMMENT     Sequence Label: MO91-dCas9-AID(P182X)
FEATURES             Location/Qualifiers
     source          1..11322
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        289..339
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     290..310
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1422..1487
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1494..1514
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5655..5675
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5691..5711
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6333..6356
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6384..6407
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6431..6457
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6458..6484
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        6582..7081
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6617..6640)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7014..7033
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7102..7701
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7102..7121)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7598..7618
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7801..8315
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    8477..8493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8533..8539,8540..8557,8558..8563))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8578..8599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8716..8733)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8887..9475)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8967..8986)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9646..10437,10438..10506))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10269..10288
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10507..10611)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10679..10697
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10735..10757)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10857..10876
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc wryrmctymk yysmaytrac gcaaatgggc
      301 ggtaggcgtg tacggtggga ggtctatata agcagagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctaccat ggactataag gaccacgacg
     1441 gagactacaa ggatcatgat attgattaca aagacgatga cgataagatg gccccaaaga
     1501 agaagcggaa ggtcggtatc cacggagtcc cagcagctac catggacaag aagtattcta
     1561 tcggactggc catcgggact aatagcgtcg ggtgggccgt gatcactgac gagtacaagg
     1621 tgccctctaa gaagttcaag gtgctcggga acaccgaccg gcattccatc aagaaaaatc
     1681 tgatcggagc tctcctcttt gattcagggg agaccgctga agcaacccgc ctcaagcgga
     1741 ctgctagacg gcggtacacc aggaggaaga accggatttg ttaccttcaa gagatattct
     1801 ccaacgaaat ggcaaaggtc gacgacagct tcttccatag gctggaagaa tcattcctcg
     1861 tggaagagga taagaagcat gaacggcatc ccatcttcgg taatatcgtc gacgaggtgg
     1921 cctatcacga gaaataccca accatctacc atcttcgcaa aaagctggtg gactcaaccg
     1981 acaaggcaga cctccggctt atctacctgg ccctggccca catgatcaag ttcagaggcc
     2041 acttcctgat cgagggcgac ctcaatcctg acaatagcga tgtggataaa ctgttcatcc
     2101 agctggtgca gacttacaac cagctctttg aagagaaccc catcaatgca agcggagtcg
     2161 atgccaaggc cattctgtca gcccggctgt caaagagccg cagacttgag aatcttatcg
     2221 ctcagctgcc gggtgaaaag aaaaatggac tgttcgggaa cctgattgct ctttcacttg
     2281 ggctgactcc caatttcaag tctaatttcg acctggcaga ggatgccaag ctgcaactgt
     2341 ccaaggacac ctatgatgac gatctcgaca acctcctggc ccagatcggt gaccaatacg
     2401 ccgacctttt ccttgctgct aagaatcttt ctgacgccat cctgctgtct gacattctcc
     2461 gcgtgaacac tgaaatcacc aaggcccctc tttcagcttc aatgattaag cggtatgatg
     2521 agcaccacca ggacctgacc ctgcttaagg cactcgtccg gcagcagctt ccggagaagt
     2581 acaaggaaat cttctttgac cagtcaaaga atggatacgc cggctacatc gacggaggtg
     2641 cctcccaaga ggaattttat aagtttatca aacctatcct tgagaagatg gacggcaccg
     2701 aagagctcct cgtgaaactg aatcgggagg atctgctgcg gaagcagcgc actttcgaca
     2761 atgggagcat tccccaccag atccatcttg gggagcttca cgccatcctt cggcgccaag
     2821 aggacttcta cccctttctt aaggacaaca gggagaagat tgagaaaatt ctcactttcc
     2881 gcatccccta ctacgtggga cccctcgcca gaggaaatag ccggtttgct tggatgacca
     2941 gaaagtcaga agaaactatc actccctgga acttcgaaga ggtggtggac aagggagcca
     3001 gcgctcagtc attcatcgaa cggatgacta acttcgataa gaacctcccc aatgagaagg
     3061 tcctgccgaa acattccctg ctctacgagt actttaccgt gtacaacgag ctgaccaagg
     3121 tgaaatatgt caccgaaggg atgaggaagc ccgcattcct gtcaggcgaa caaaagaagg
     3181 caattgtgga ccttctgttc aagaccaata gaaaggtgac cgtgaagcag ctgaaggagg
     3241 actatttcaa gaaaattgaa tgcttcgact ctgtggagat tagcggggtc gaagatcggt
     3301 tcaacgcaag cctgggtacc taccatgatc tgcttaagat catcaaggac aaggattttc
     3361 tggacaatga ggagaaagag gacatccttg aggacattgt cctgactctc actctgttcg
     3421 aggaccggga aatgatcgag gagaggctta agacctacgc ccatctgttc gacgataaag
     3481 tgatgaagca acttaaacgg agaagatata ccggatgggg acgccttagc cgcaaactca
     3541 tcaacggaat ccgggacaaa cagagcggaa agaccattct tgatttcctt aagagcgacg
     3601 gattcgctaa tcgcaacttc atgcaactta tccatgatga ttccctgacc tttaaggagg
     3661 acatccagaa ggcccaagtg tctggacaag gtgactcact gcacgagcat atcgcaaatc
     3721 tggctggttc acccgctatt aagaagggta ttctccagac cgtgaaagtc gtggacgagc
     3781 tggtcaaggt gatgggtcgc cataaaccag agaacattgt catcgagatg gccagggaaa
     3841 accagactac ccagaaggga cagaagaaca gcagggagcg gatgaaaaga attgaggaag
     3901 ggattaagga gctcgggtca cagatcctta aagagcaccc ggtggaaaac acccagcttc
     3961 agaatgagaa gctctatctg tactaccttc aaaatggacg cgatatgtat gtggaccaag
     4021 agcttgatat caacaggctc tcagactacg acgtggacgc catcgtccct cagagcttcc
     4081 tcaaagacga ctcaattgac aataaggtgc tgactcgctc agacaagaac cggggaaagt
     4141 cagataacgt gccctcagag gaagtcgtga aaaagatgaa gaactattgg cgccagcttc
     4201 tgaacgcaaa gctgatcact cagcggaagt tcgacaatct cactaaggct gagaggggcg
     4261 gactgagcga actggacaaa gcaggattca ttaaacggca acttgtggag actcggcaga
     4321 ttactaaaca tgtagcccaa atccttgact cacgcatgaa taccaagtac gacgaaaacg
     4381 acaaacttat ccgcgaggtg aaggtgatta ccctgaagtc caagctggtc agcgatttca
     4441 gaaaggactt tcaattctac aaagtgcggg agatcaataa ctatcatcat gctcatgacg
     4501 catatctgaa tgccgtggtg ggaaccgccc tgatcaagaa gtacccaaag ctggaaagcg
     4561 agttcgtgta cggagactac aaggtctacg acgtgcgcaa gatgattgcc aaatctgagc
     4621 aggagatcgg aaaggccacc gcaaagtact tcttctacag caacatcatg aatttcttca
     4681 agaccgaaat cacccttgca aacggtgaga tccggaagag gccgctcatc gagactaatg
     4741 gggagactgg cgaaatcgtg tgggacaagg gcagagattt cgctaccgtg cgcaaagtgc
     4801 tttctatgcc tcaagtgaac atcgtgaaga aaaccgaggt gcaaaccgga ggcttttcta
     4861 aggaatcaat cctccccaag cgcaactccg acaagctcat tgcaaggaag aaggattggg
     4921 accctaagaa gtacggcgga ttcgattcac caactgtggc ttattctgtc ctggtcgtgg
     4981 ctaaggtgga aaaaggaaag tctaagaagc tcaagagcgt gaaggaactg ctgggtatca
     5041 ccattatgga gcgcagctcc ttcgagaaga acccaattga ctttctcgaa gccaaaggtt
     5101 acaaggaagt caagaaggac cttatcatca agctcccaaa gtatagcctg ttcgaactgg
     5161 agaatgggcg gaagcggatg ctcgcctccg ctggcgaact tcagaagggt aatgagctgg
     5221 ctctcccctc caagtacgtg aatttcctct accttgcaag ccattacgag aagctgaagg
     5281 ggagccccga ggacaacgag caaaagcaac tgtttgtgga gcagcataag cattatctgg
     5341 acgagatcat tgagcagatt tccgagtttt ctaaacgcgt cattctcgct gatgccaacc
     5401 tcgataaagt ccttagcgca tacaataagc acagagacaa accaattcgg gagcaggctg
     5461 agaatatcat ccacctgttc accctcacca atcttggtgc ccctgccgca ttcaagtact
     5521 tcgacaccac catcgaccgg aaacgctata cctccaccaa agaagtgctg gacgccaccc
     5581 tcatccacca gagcatcacc ggactttacg aaactcggat tgacctctca cagctcggag
     5641 gggatgaggg agctcccaag aaaaagcgca aggtaggtag ttccggatct ccgaaaaaga
     5701 aacgcaaagt tggtagtgat gctttagacg attttgactt agatatgctt ggttcagacg
     5761 cgttagacga cttcggtgga ggatccatgg acagcctctt gatgaaccgg aggaagtttc
     5821 tttaccaatt caaaaatgtc cgctgggcta agggtcggcg tgagacctac ctgtgctacg
     5881 tagtgaagag gcgtgacagt gctacatcct tttcactgga ctttggttat cttcgcaata
     5941 agaacggctg ccacgtggaa ttgctcttcc tccgctacat ctcggactgg gacctagacc
     6001 ctggccgctg ctaccgcgtc acctggttca cctcctggag cccctgctac gactgtgccc
     6061 gacatgtggc cgactttctg cgagggaacc ccaacctcag tctgaggatc ttcaccgcgc
     6121 gcctctactt ctgtgaggac cgcaaggctg agcccgaggg gctgcggcgg ctgcaccgcg
     6181 ccggggtgca aatagccatc atgaccttca aagattattt ttactgctgg aatacttttg
     6241 tagaaaacca tgaaagaact ttcaaagcct gggaagggct gcatgaaaat tcagttcgtc
     6301 tctccagaca gcttcggcgc atccttttgc ccgattacaa agacgatgac gataagtgac
     6361 tcgagcacgt acgagcggcc gctgactaca aggacgacga tgacaagtga gaattgtcga
     6421 acgggccgct tacccatacg atgttccgga ttacgcttac ccatatgatg ttccggatta
     6481 cgcttaacta gagtgagggt ccccacctgg gacccttgag agtatcaggt ctcccacgtg
     6541 ggagacaaga aatccctgtt taatatttaa cgaattctac cgggtagggg aggcgctttt
     6601 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     6661 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     6721 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     6781 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     6841 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     6901 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     6961 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     7021 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     7081 gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     7141 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     7201 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     7261 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     7321 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     7381 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     7441 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     7501 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     7561 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     7621 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     7681 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     7741 gcacgacccc atgcatcgat aaaataaaag attttattta gtctccagaa aaagggggga
     7801 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca
     7861 tggaaaatac ataactgaga atagagaagt tcagatcaag gttaggaaca gagagacagc
     7921 agaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     7981 aacagatggt ccccagatgc ggtcccgccc tcagcagttt ctagagaacc atcagatgtt
     8041 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
     8101 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
     8161 cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg tgtatccaat
     8221 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct
     8281 gagtgattga ctacccgtca gcgggggtct ttcatgggta acagtttctt gaagttggag
     8341 aacaacattc tgagggtagg agtcgaatat taagtaatcc tgactcaatt agccactgtt
     8401 ttgaatccac atactccaat actcctgaaa tagttcatta tggacagcgc agaaagagct
     8461 ggggagaatt gtgaaattgt tatccgctca caattccaca cwryrwamkw gyyrkmmgcw
     8521 yamartkcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8581 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8641 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8701 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8761 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8821 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8881 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8941 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9001 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9061 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9121 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9181 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9241 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9301 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9361 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9421 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9481 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9541 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9601 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9661 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9721 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9781 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9841 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9901 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9961 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10021 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10081 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10141 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10201 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10261 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10321 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10381 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10441 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10501 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10561 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10621 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    10681 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    10741 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    10801 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    10861 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    10921 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
    10981 gcgatcggtg cgggcctctt cgctattacg ccagcgcgtt gacattgatt attgactagt
    11041 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
    11101 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
    11161 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
    11221 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
    11281 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct gg
//
