LOCUS       sequence-177707-       16137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeted CpG methylation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16137)
  AUTHORS   Lei Y, Zhang X, Su J, Jeong M, Gundry MC, Huang YH, Zhou Y, Li W, 
            Goodell MA
  TITLE     Targeted DNA methylation in vivo using an engineered dCas9-MQ1 
            fusion protein.
  JOURNAL   Nat Commun. 2017 Jul 11;8:16026. doi: 10.1038/ncomms16026.
  PUBMED    28695892
REFERENCE   2  (bases 1 to 16137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-07-11"; volume: "8:16026. doi"; pages: " 
            10.1038/ncomms16026"
COMMENT     Sequence Label: pLV hUbC-dCas9-dMQ1-EGFG
FEATURES             Location/Qualifiers
     source          1..16137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        68..1279
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1116..1135
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1308..1317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1317..1382
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1389..1409
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1419..1445,1446..1448,1449..3932,3933..3938,3939..4004,
                     4005..4007,4008..5522)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1419..1445,1446..1448,1449..3935,3936..3938,
                     3939..5522)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1419..5522
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5535..5555
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6726..6779
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6786..6788,6789..7499)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6786..6788,6789..7499)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6828..6849)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7089..7108)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7436..7457
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7515..7548)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7573..8161
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7626..7646)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8044..8055)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8164..8180)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8165..8181)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8686..8866
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8892..8909)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8898..9122
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9168..9596
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9255..9274)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9465..9486
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9605..9625)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9910..10120
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9932..10128
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9979..10114
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10041..10060
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10176..10223
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10242..10616
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNYVHFVAEEQD"
     polyA_signal    10746..10867
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10783..10802)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10837..10856
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10916..10932)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10940..10956
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10964..10970,10971..10988,10989..10994))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11009..11030
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11147..11164)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11318..11906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11398..11417)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12077..12868,12869..12937))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12700..12719
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12938..13042)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13117..13136)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        13308..13687
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13689..13887
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     13842..13862
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             13905..14085
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    14132..14257
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    14750..14983
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             15168..15212
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    15510..15627
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(15672..15705)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(15692..16018)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGATTVSY
                     HNFV"
     primer_bind     15868..15890
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15882..15899
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15883..15899
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15909..15928
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15909..15927
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(15975..15995)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(15975..15993)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(16014..16030)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    16038..16054
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(16062..16068,16069..16086,16087..16092))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16107..16128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgctcaatgc ccgctttcca ctgcatgacg tctccacaat taattaaccc gtgtcggctc
       61 cagatctggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg
      121 agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc
      181 aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac
      241 attttaggac gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca
      301 ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa
      361 cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga
      421 tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg
      481 ctgggctggc cggggctttc gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag
      541 agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact gggggttggg
      601 gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg
      661 gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc caaggtcttg
      721 aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg caccatctgg
      781 ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg
      841 cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg
      901 tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt
      961 aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct
     1021 cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct
     1081 ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg
     1141 ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt
     1201 tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat tctggccgtt
     1261 tttggctttt ttgttagacg aagcttgggc tgcaggtcga ctctagagcc accatggact
     1321 acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca
     1381 agatggcccc caagaagaag aggaaggtgg gccgcggaat ggacaagaag tactccattg
     1441 ggctcgccat cggcacaaac agcgtcggct gggccgtcat tacggacgag tacaaggtgc
     1501 cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca cagcataaag aagaacctca
     1561 ttggcgccct cctgttcgac tccggggaaa ccgccgaagc cacgcggctc aaaagaacag
     1621 cacggcgcag atatacccgc agaaagaatc ggatctgcta cctgcaggag atctttagta
     1681 atgagatggc taaggtggat gactctttct tccataggct ggaggagtcc tttttggtgg
     1741 aggaggataa aaagcacgag cgccacccaa tctttggcaa tatcgtggac gaggtggcgt
     1801 accatgaaaa gtacccaacc atatatcatc tgaggaagaa gcttgtagac agtactgata
     1861 aggctgactt gcggttgatc tatctcgcgc tggcgcatat gatcaaattt cggggacact
     1921 tcctcatcga gggggacctg aacccagaca acagcgatgt cgacaaactc tttatccaac
     1981 tggttcagac ttacaatcag cttttcgaag agaacccgat caacgcatcc ggagttgacg
     2041 ccaaagcaat cctgagcgct aggctgtcca aatcccggcg gctcgaaaac ctcatcgcac
     2101 agctccctgg ggagaagaag aacggcctgt ttggtaatct tatcgccctg tcactcgggc
     2161 tgacccccaa ctttaaatct aacttcgacc tggccgaaga tgccaagctt caactgagca
     2221 aagacaccta cgatgatgat ctcgacaatc tgctggccca gatcggcgac cagtacgcag
     2281 accttttttt ggcggcaaag aacctgtcag acgccattct gctgagtgat attctgcgag
     2341 tgaacacgga gatcaccaaa gctccgctga gcgctagtat gatcaagcgc tatgatgagc
     2401 accaccaaga cttgactttg ctgaaggccc ttgtcagaca gcaactgcct gagaagtaca
     2461 aggaaatttt cttcgatcag tctaaaaatg gctacgccgg atacattgac ggcggagcaa
     2521 gccaggagga attttacaaa tttattaagc ccatcttgga aaaaatggac ggcaccgagg
     2581 agctgctggt aaagcttaac agagaagatc tgttgcgcaa acagcgcact ttcgacaatg
     2641 gaagcatccc ccaccagatt cacctgggcg aactgcacgc tatcctcagg cggcaagagg
     2701 atttctaccc ctttttgaaa gataacaggg aaaagattga gaaaatcctc acatttcgga
     2761 taccctacta tgtaggcccc ctcgcccggg gaaattccag attcgcgtgg atgactcgca
     2821 aatcagaaga gaccatcact ccctggaact tcgaggaagt cgtggataag ggggcctctg
     2881 cccagtcctt catcgaaagg atgactaact ttgataaaaa tctgcctaac gaaaaggtgc
     2941 ttcctaaaca ctctctgctg tacgagtact tcacagttta taacgagctc accaaggtca
     3001 aatacgtcac agaagggatg agaaagccag cattcctgtc tggagagcag aagaaagcta
     3061 tcgtggacct cctcttcaag acgaaccgga aagttaccgt gaaacagctc aaagaagact
     3121 atttcaaaaa gattgaatgt ttcgactctg ttgaaatcag cggagtggag gatcgcttca
     3181 acgcatccct gggaacgtat cacgatctcc tgaaaatcat taaagacaag gacttcctgg
     3241 acaatgagga gaacgaggac attcttgagg acattgtcct cacccttacg ttgtttgaag
     3301 atagggagat gattgaagaa cgcttgaaaa cttacgctca tctcttcgac gacaaagtca
     3361 tgaaacagct caagaggcgc cgatatacag gatgggggcg gctgtcaaga aaactgatca
     3421 atgggatccg agacaagcag agtggaaaga caatcctgga ttttcttaag tccgatggat
     3481 ttgccaaccg gaacttcatg cagttgatcc atgatgactc tctcaccttt aaggaggaca
     3541 tccagaaagc acaagtttct ggccaggggg acagtcttca cgagcacatc gctaatcttg
     3601 caggtagccc agctatcaaa aagggaatac tgcagaccgt taaggtcgtg gatgaactcg
     3661 tcaaagtaat gggaaggcat aagcccgaga atatcgttat cgagatggcc cgagagaacc
     3721 aaactaccca gaagggacag aagaacagta gggaaaggat gaagaggatt gaagagggta
     3781 taaaagaact ggggtcccaa atccttaagg aacacccagt tgaaaacacc cagcttcaga
     3841 atgagaagct ctacctgtac tacctgcaga acggcaggga catgtacgtg gatcaggaac
     3901 tggacatcaa tcggctctcc gactacgacg tggatgccat cgtgccccag tcttttctca
     3961 aagatgattc tattgataat aaagtgttga caagatccga taaaaataga gggaagagtg
     4021 ataacgtccc ctcagaagaa gttgtcaaga aaatgaaaaa ttattggcgg cagctgctga
     4081 acgccaaact gatcacacaa cggaagttcg ataatctgac taaggctgaa cgaggtggcc
     4141 tgtctgagtt ggataaagcc ggcttcatca aaaggcagct tgttgagaca cgccagatca
     4201 ccaagcacgt ggcccaaatt ctcgattcac gcatgaacac caagtacgat gaaaatgaca
     4261 aactgattcg agaggtgaaa gttattactc tgaagtctaa gctggtctca gatttcagaa
     4321 aggactttca gttttataag gtgagagaga tcaacaatta ccaccatgcg catgatgcct
     4381 acctgaatgc agtggtaggc actgcactta tcaaaaaata tcccaagctt gaatctgaat
     4441 ttgtttacgg agactataaa gtgtacgatg ttaggaaaat gatcgcaaag tctgagcagg
     4501 aaataggcaa ggccaccgct aagtacttct tttacagcaa tattatgaat tttttcaaga
     4561 ccgagattac actggccaat ggagagattc ggaagcgacc acttatcgaa acaaacggag
     4621 aaacaggaga aatcgtgtgg gacaagggta gggatttcgc gacagtccgg aaggtcctgt
     4681 ccatgccgca ggtgaacatc gttaaaaaga ccgaagtaca gaccggaggc ttctccaagg
     4741 aaagtatcct cccgaaaagg aacagcgaca agctgatcgc acgcaaaaaa gattgggacc
     4801 ccaagaaata cggcggattc gattctccta cagtcgctta cagtgtactg gttgtggcca
     4861 aagtggagaa agggaagtct aaaaaactca aaagcgtcaa ggaactgctg ggcatcacaa
     4921 tcatggagcg atcaagcttc gaaaaaaacc ccatcgactt tctcgaggcg aaaggatata
     4981 aagaggtcaa aaaagacctc atcattaagc ttcccaagta ctctctcttt gagcttgaaa
     5041 acggccggaa acgaatgctc gctagtgcgg gcgagctgca gaaaggtaac gagctggcac
     5101 tgccctctaa atacgttaat ttcttgtatc tggccagcca ctatgaaaag ctcaaagggt
     5161 ctcccgaaga taatgagcag aagcagctgt tcgtggaaca acacaaacac taccttgatg
     5221 agatcatcga gcaaataagc gaattctcca aaagagtgat cctcgccgac gctaacctcg
     5281 ataaggtgct ttctgcttac aataagcaca gggataagcc catcagggag caggcagaaa
     5341 acattatcca cttgtttact ctgaccaact tgggcgcgcc tgcagccttc aagtacttcg
     5401 acaccaccat agacagaaag cggtacacct ctacaaagga ggtcctggac gccacactga
     5461 ttcatcagtc aattacgggg ctctatgaaa caagaatcga cctctctcag ctcggtggag
     5521 acagcagggc tgaccccaag aagaagagga aggtggctag ggacagcaaa gtggagaaca
     5581 aaacaaagaa gctgagagtg ttcgaagcct tcgccggcat tggcgcccag agaaaggccc
     5641 tggagaaagt gaggaaggac gagtacgaga tcgtgggact ggccgagtgg tatgtgcccg
     5701 ccatcgtcat gtaccaggcc atccataaca acttccacac caagctcgag tacaagtccg
     5761 tcagcagaga ggagatgatc gactacctcg agaacaagac cctgtcctgg aacagcaaga
     5821 accccgtcag caacggatac tggaagagga agaaggatga cgagctgaag atcatctaca
     5881 acgccatcaa gctgtccgaa aaggagggca acattttcga catcagggac ctctacaaga
     5941 ggacactgaa gaacatcgac ctgctcacct acagcttccc ttcccaggac ctgagccagc
     6001 aaggcatcca gaagggcatg aagaggggaa gcggcaccag atccggcctg ctctgggaga
     6061 tcgaaagagc cctggactcc accgagaaga acgacctgcc taagtacctc ctcatggaga
     6121 acgtgggagc cctgctgcac aagaagaacg aggaggagct gaaccaatgg aagcagaagc
     6181 tggagagcct gggctaccag aacagcatcg aagtcctcaa tgctgccgat ttcggatcca
     6241 gccaggccag gaggagagtg ttcatgatct ccaccctcaa tgagttcgtg gaactgccta
     6301 agggcgacaa gaagcccaag agcatcaaaa aggtgctgaa caagatcgtg agcgagaagg
     6361 acatcctcaa caacctgctg aaatacaacc tcaccgaatt caagaagacc aagtccaaca
     6421 tcaacaaggc cagcctgatc ggctactcca agttcaactc cgagggctat gtgtacgacc
     6481 ccgagttcac aggccccaca ctgacagctg ccggcgccaa ctccaggatc aagatcaagg
     6541 acggcagcaa catcaggaag atgaacagcg acgagacctt cctgtacatc ggctttgaca
     6601 gccaggacgg caagagagtg aacgaaatcg agttcctgac cgagaaccag aagatcttcg
     6661 tgtgtggcaa cagcatcagc gtggaggtgc tggaggccat cattgacaag atcggcggcc
     6721 ctagcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggccctg
     6781 gtaccgtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     6841 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     6901 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     6961 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     7021 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     7081 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     7141 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     7201 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     7261 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     7321 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     7381 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     7441 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaaga
     7501 ccggttgata atagataact tcgtatagca tacattatac gaagttatga attcgatatc
     7561 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     7621 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     7681 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     7741 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7801 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7861 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7921 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     7981 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     8041 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     8101 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     8161 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
     8221 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     8281 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     8341 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     8401 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     8461 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     8521 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     8581 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     8641 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     8701 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8761 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     8821 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     8881 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     8941 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     9001 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     9061 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     9121 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     9181 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     9241 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     9301 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     9361 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     9421 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     9481 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     9541 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     9601 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     9661 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     9721 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     9781 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggcgca
     9841 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     9901 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     9961 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
    10021 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
    10081 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
    10141 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
    10201 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
    10261 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
    10321 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
    10381 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
    10441 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
    10501 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
    10561 ccctgcgcga cccggccggc aactacgtgc acttcgtggc cgaggagcag gactgacacg
    10621 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
    10681 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
    10741 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    10801 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    10861 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
    10921 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    10981 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    11041 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    11101 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    11161 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    11221 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    11281 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    11341 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    11401 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    11461 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    11521 actgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    11581 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    11641 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    11701 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    11761 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    11821 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    11881 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    11941 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    12001 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    12061 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    12121 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    12181 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    12241 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    12301 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    12361 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    12421 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    12481 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    12541 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    12601 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    12661 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    12721 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    12781 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    12841 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    12901 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    12961 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    13021 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
    13081 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
    13141 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
    13201 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
    13261 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
    13321 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
    13381 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
    13441 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
    13501 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
    13561 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
    13621 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
    13681 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
    13741 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
    13801 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
    13861 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
    13921 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    13981 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    14041 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
    14101 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
    14161 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
    14221 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
    14281 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
    14341 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
    14401 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
    14461 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
    14521 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
    14581 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
    14641 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
    14701 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
    14761 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
    14821 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
    14881 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
    14941 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
    15001 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
    15061 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
    15121 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
    15181 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
    15241 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
    15301 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
    15361 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
    15421 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
    15481 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
    15541 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
    15601 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
    15661 tggttaatta aataacttcg tatagcatac attatacgaa gttatgataa gagacggtgg
    15721 tggcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc
    15781 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
    15841 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
    15901 agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcc
    15961 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
    16021 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    16081 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttg
//
