LOCUS       sequence-177726-        7944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Orange color variant of bright luminescent protein, fused to 
            Lysosomal-associated membrane protein 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Suzuki K, Kimura T, Shinoda H, Bai G, Daniels MJ, Arai Y, Nakano M, 
            Nagai T
  TITLE     Five colour variants of bright luminescent protein for real-time 
            multicolour bioimaging.
  JOURNAL   Nat Commun. 2016 Dec 14;7:13718. doi: 10.1038/ncomms13718.
  PUBMED    27966527
REFERENCE   2  (bases 1 to 7944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-12-14"; volume: "7:13718. doi"; pages: " 
            10.1038/ncomms13718"
COMMENT     Sequence Label: LAMP-OeNL_pcDNA3
FEATURES             Location/Qualifiers
     source          1..7944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        121..500
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        501..704
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     655..675
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     749..768
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        749..767
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      782..791
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      786..795
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(788..850,851..2008)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     CDS             2300..2950
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             2300..2950
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     promoter        complement(3383..3401)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3384..3401)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3421..3438)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3427..3651
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3697..4125
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3784..3803)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3994..4015
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4134..4154)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4139..4468
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4319..4454
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4381..4400
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4535..5329
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4589..4608)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5199..5218
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5503..5624
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5540..5559)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5594..5613
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5673..5689)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5673..5689)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5686..5708)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5697..5713
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5721..5727,5728..5745,5746..5751))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5766..5787
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5904..5921)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6075..6663)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6155..6174)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6834..7625,7626..7694))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7457..7476
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7695..7799)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7874..7893)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
       61 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
      121 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      181 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      241 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      301 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      361 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      421 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      481 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      541 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      601 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      661 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      721 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      781 cgccaccatg gcggccccgg gcgcccggcg tccgctgctc ctgttgctgc tggcaggcct
      841 tgcacacagc gccccagcac tgttcgaggt gaaagacaac aacggtacag cgtgtataat
      901 ggccagcttc tctgcctcct ttctgaccac ctatgaggct ggacatgttt ctaaggtctc
      961 gaatatgacc ctgccagcct ctgcagaagt cctgaagaat agcagctctt gtggtgaaaa
     1021 gaatgcttct gagcccaccc tcgcaatcac ctttggagaa ggatatttac tgaaactcac
     1081 cttcacaaaa aacacaacac gttacagtgt ccagcacatg tatttcacat ataacctgtc
     1141 agacacacaa ttctttccca atgccagctc caaagggccc gacactgtgg attccacaac
     1201 tgacatcaag gcagacatca acaaaacata ccgatgtgtc agcgacatca gggtctacat
     1261 gaagaatgtg accattgtgc tctgggacgc tactatccag gcctacctgc cgagtagcaa
     1321 cttcagcaag gaagagacac gctgcccaca ggatcaacct tccccaacta ctgggccacc
     1381 cagcccctca ccaccacttg tgcccacaaa ccccagtgtg tccaagtaca atgtgactgg
     1441 tgacaatgga acctgcctgc tggcctctat ggcactgcaa ctcaacatca cctacatgaa
     1501 gaaggacaac acgactgtga ccagagcatt caacatcaac ccaagtgaca aatatagtgg
     1561 gacttgcggt gcccagttgg tgaccctgaa ggtggggaac aagagcagag tcctggagct
     1621 gcagtttggg atgaatgcca cttctagcct gtttttcctg caaggagttc agttgaacat
     1681 gactcttcct gatgccatag agcccacgtt cagcacctcc aactattccc tgaaagctct
     1741 tcaggccagt gtcggcaact catacaagtg caacagtgag gagcacatct ttgtcagcaa
     1801 ggcgctcgcc ctcaatgtct tcagcgtgca agtccaggct ttcagggtag aaagtgacag
     1861 gtttgggtct gtggaagagt gtgtacagga cggtaacaac atgctgatcc ccattgctgt
     1921 gggcggggcc ctggcagggc tggtcctcat cgtcctcatc gcctacctca tcggcaggaa
     1981 gaggagtcac gcgggctatc agaccatcgg taccggcagc ggtggcggtg gcagcggtgg
     2041 cggtggcagc ggtggatcca tggtcttcac actcgaagat ttcgttgggg actggcgaca
     2101 gacagccggc tacaacctgg accaagtcct tgaacaggga ggtgtgtcca gtttgtttca
     2161 gaatctcggg gtgtccgtaa ctccgatcca aaggattgtc ctgagcggtc tcgagggcgg
     2221 tagcggtggc agcgtgagcg tgatcaagcc cgagatgaag aaggtggagg acgccgtggc
     2281 ccactccact ctcgagggcg tgagcgtgat caagcccgag atgaagatga ggtactacat
     2341 ggacggctcc gtcaatgggc atgagttcac aatcgagggt gagggcacag gcagacctta
     2401 cgagggacat caggagatga cactgcgcgt cacaatggcc gagggcgggc caatgccttt
     2461 cgccttcgac ctggtgtccc acgtgttctg ttacggccac agagttttta ctaaatatcc
     2521 agaagagatc ccagactatt tcaagcaggc ctttcctgag ggcctgtcct gggagaggtc
     2581 cctggagttc gaggacggcg gctccgcctc cgtgagcgcc cacatcagcc tgaggggcaa
     2641 caccttctac cacaagtcca agttcaccgg cgtgaacttc cccgccgacg gccccatcat
     2701 gcagaaccag agcgtggact gggagccctc caccgagaag atcaccgcca gcgacggcgt
     2761 gctgaagggc gacgtgacca tgtacctgaa gctggagggc ggcggcaacc acaagtgcca
     2821 gatgaagacc acctacaagg ccgccaagga gatcctggag atgcccggcg accactacat
     2881 cggccacagg ctggtgagga agaccgaggg caacatcacc gagcaggtgg aggacgccgt
     2941 ggcccactcc gagctcgaaa atgggctgaa gatcgacatc catgtcatca tcccgtatga
     3001 aggtctgagc ggcgaccaaa tgggccagat cgaaaaaatt tttaaggtgg tgtaccctgt
     3061 ggatgatcat cactttaagg tgatcctgca ctatggcaca ctggtaatcg acggggttac
     3121 gccgaacatg atcgactatt tcggacggcc gtatgaaggc atcgccgtgt tcgacggcaa
     3181 aaagatcact gtaacaggga ccctgtggaa cggcaacaaa attatcgacg agcgcctgat
     3241 caaccccgac ggctccctgc tgttccgagt aaccatcaac ggagtgaccg gctggcggct
     3301 gtgcgaacgc attctggcgt aagaattctg cagatatcca tcacactggc ggccgctcga
     3361 gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag
     3421 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     3481 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     3541 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     3601 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     3661 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     3721 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     3781 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3841 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3901 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     3961 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4021 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     4081 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     4141 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4201 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     4261 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4321 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4381 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     4441 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     4501 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     4561 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     4621 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     4681 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     4741 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     4801 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     4861 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     4921 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     4981 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     5041 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     5101 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     5161 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     5221 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     5281 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     5341 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     5401 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     5461 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     5521 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5581 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     5641 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     5701 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     5761 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5821 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5881 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5941 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6001 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6061 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6121 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6181 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6241 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6301 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6361 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6421 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6481 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6541 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6601 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6661 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6721 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6781 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6841 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6901 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6961 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7021 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7081 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7141 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7201 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7261 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7321 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7381 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7441 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7501 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7561 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7621 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7681 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7741 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7801 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     7861 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     7921 cttgtgtgtt ggaggtcgct gagt
//
