LOCUS       sequence-177733-       10281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For nucleus-specific RFP labeling in single cells, pK098 should be 
            used with pK031..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10281)
  AUTHORS   Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T
  TITLE     Supernova: A Versatile Vector System for Single-Cell Labeling and 
            Gene Function Studies in vivo.
  JOURNAL   Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
  PUBMED    27775045
REFERENCE   2  (bases 1 to 10281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-24"; volume: "6:35747. doi"; pages: " 
            10.1038/srep35747"
COMMENT     Sequence Label: pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WP...
FEATURES             Location/Qualifiers
     source          1..10281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        392..667
                     /label=chicken beta-actin promoter
     intron          668..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1733..1766)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          2300..2365
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2495..2515
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2940..3074
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2977..2996)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3031..3050
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3116..3149)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3165..3185
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3189..3899
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDM
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     primer_bind     complement(4100..4117)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4327..4346
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4499..5116,5117..5122,5123..5158,5159..5161,5162..5197,
                     5198..5200,5201..5236,5237..5245)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5332..5352)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5750..5761)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5890..5909)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5955..6010
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6009..6028)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(6394..6982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6474..6493)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7311..7358
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7383..7402
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7590..8384)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(7590..8384)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7701..7720)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8311..8330
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(8483..8502)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        8581..8772
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8729..8749
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(8832..9623,9624..9692))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9455..9474
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9693..9797)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9824..10279
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9911..9930)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10121..10142
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg gtcgaggtga gccccacgtt ctgcttcact
      421 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
      481 tgtgcagcga tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga
      541 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
      601 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
      661 gcgggcggga gtcgctgcgt tgccttcgcc ccgtgccccg ctccgcgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctc gtttcttttc tgtggctgcg
      841 tgaaagcctt aaagggctcc gggagggccc tttgtgcggg ggggagcggc tcggggggtg
      901 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggccc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcgtg tgcgcgaggg gagcgcggcc
     1021 gggggcggtg ccccgcggtg cgggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcggcggt cgggctgtaa cccccccctg
     1141 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtgcgggg
     1201 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     1261 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctggc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccatc tccagcctcg gggctgccgc
     1561 agggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc taataacttc
     1741 gtatagcata cattatacga agttatatta agggttccgg atcctcgggg acaccaaata
     1801 tggcgatctc ggccttttcg tttcttggag ctgggacatg tttgccatcg atccatctac
     1861 caccagaacg gccgttagat ctgctgccac cgttgtttcc accgaagaaa ccaccgttgc
     1921 cgtaaccacc acgacggttg ttgctaaaga agctgccacc gccacggcca ccgttgtagc
     1981 cgccgttgtt gttattgtag ttgctactgt tatttctggc acttcttggt tttcctctta
     2041 agtgaggagg aacataacca ttctcgttgt tgtcgttgat gcttaaattt tgcacttgtt
     2101 cgctcagttc agccataata tgaaatgctt ttcttgttgt tcttacggaa taccacttgc
     2161 cacctatcac cacaactaac tttttcccgt tcctccatct cttttatatt ttttttctcg
     2221 agggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
     2281 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
     2341 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
     2401 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
     2461 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
     2521 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
     2581 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
     2641 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
     2701 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
     2761 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
     2821 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     2881 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     2941 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3001 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3061 atcatgtctg gatctgacat ggtaagtaag cttgggctgc aggtcgaggg acctaataac
     3121 ttcgtatagc atacattata cgaagttata tgtcgaccat gggcccaaag aagaagagaa
     3181 aggtttcggt gtctaagggc gaagagctga ttaaggagaa catgcacatg aagctgtaca
     3241 tggagggcac cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct
     3301 acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct
     3361 tcgacatcct ggctaccagc ttcatgtacg gcagcagaac cttcatcaac cacacccagg
     3421 gcatccccga cttctttaag cagtccttcc ctgagggctt cacatgggag agagtcacca
     3481 catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc
     3541 tcatctacaa cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga
     3601 agaaaacact cggctgggag gccaacaccg agatgctgta ccccgctgac ggcggcctgg
     3661 aaggcagaag cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttca
     3721 agaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg
     3781 tggaccacag actggaaaga atcaaggagg ccgacaaaga gacctacgtc gagcagcacg
     3841 aggtggctgt ggccagatac tgcgacctcc ctagcaaact ggggcacaaa cttaattgaa
     3901 gatatcctag atcgagttaa ttaaggattc cgcccctctc cctccccccc ccctaacgtt
     3961 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     4021 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     4081 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     4141 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     4201 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     4261 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     4321 gtaaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     4381 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     4441 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg tttcctttga aaaacacgat
     4501 gtctagactg gacaagagca aagtcataaa ctctgctctg gaattactca atgaagtcgg
     4561 tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct
     4621 gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctggcaatcg agatgctgga
     4681 caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa
     4741 caacgccaag tcattccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct
     4801 cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg
     4861 tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac
     4921 actgggctgc gtattggagg atcaggagca tcaagtagca aaagaggaaa gagagacacc
     4981 taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg accatcaggg
     5041 agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct
     5101 aaagtgcgaa agcggcgggc cggccgacgc ccttgacgat tttgacttag acatgctccc
     5161 agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt
     5221 tgaccttgac atgctccccg ggtaactaag taagcggccg cgaattcgcc cttcggccgc
     5281 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5341 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5401 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5461 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5521 ttggggcatt gccaccacct gtcagctcct ttccggaact ttcgctttcc ccctccctat
     5581 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     5641 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     5701 ctatgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     5761 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     5821 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcagc ggccgcggcg
     5881 actcgaggat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     5941 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     6001 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     6061 tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct
     6121 ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa
     6181 agccttgact tgaggttaga tttttttata ttttgttttg tgttattttt ttctttaaca
     6241 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     6301 ccagtcatag ctgtccctct tctcttatga agccatgtga gcaaaaggcc agcaaaaggc
     6361 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6421 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6481 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6541 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6601 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6661 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6721 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6781 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6841 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6901 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6961 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7021 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7081 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaacc
     7141 tgaggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga
     7201 acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct
     7261 cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga
     7321 cccaacaccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg
     7381 tattgtctcc ttccgtgttt cagttagcct ccccctaggg tgggcgaaga actccagcat
     7441 gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa
     7501 cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg
     7561 gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     7621 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     7681 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     7741 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     7801 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc
     7861 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     7921 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     7981 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     8041 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     8101 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     8161 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca
     8221 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     8281 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     8341 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     8401 cctgtctctt gatcgatctt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     8461 ctggaatagc tcagaggccg ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     8521 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     8581 gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     8641 cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg
     8701 cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca cagctggttc
     8761 tttccgcctc aggactcttc ctttttcaat aaatcaatct aaagtatata tgagtaaact
     8821 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8881 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8941 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9001 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9061 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9121 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9181 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9241 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9301 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9361 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9421 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9481 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9541 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9601 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9661 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9721 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9781 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc
     9841 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     9901 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     9961 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
    10021 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
    10081 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    10141 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    10201 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
    10261 atattaacgc ttacaattta c
//
