LOCUS       sequence-177753-       10563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector to introduce an osTir1 expressing cassette at the 
            mouse Rosa26 locus using Neomycine selection. Auxin-inducible degron
            system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10563)
  AUTHORS   Nora EP, Goloborodko A, Valton AL, Gibcus JH, Uebersohn A, Abdennur 
            N, Dekker J, Mirny LA, Bruneau BG
  TITLE     Targeted Degradation of CTCF Decouples Local Insulation of 
            Chromosome Domains from Genomic Compartmentalization.
  JOURNAL   Cell. 2017 May 18;169(5):930-944.e22. doi: 
            10.1016/j.cell.2017.05.004.
  PUBMED    28525758
REFERENCE   2  (bases 1 to 10563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.05"; journalName: "Cell"; date: "2017-05-18- 
            18"; volume: "169"; issue: "5"; pages: "930-944.e22"
COMMENT     Sequence Label: pEN113 -
            pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-b...
FEATURES             Location/Qualifiers
     source          1..10563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..657
                     /label=chicken beta-actin promoter
     intron          658..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1760..3484
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             3485..3526
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(3617..3636)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3682..3737
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3736..3755)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4098..4114)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4122..4138
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4146..4152,4153..4170,4171..4176))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4191..4212
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    4276..4309
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        4373..4872
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4408..4431)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4805..4824
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        4884..4931
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4950..5753
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5013..5032)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5623..5642
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5785..5802)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5791..6015
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6070..6103
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        complement(6929..6947)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6968..6984)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6992..7008
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7016..7022,7023..7040,7041..7046))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7061..7082
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7199..7216)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7370..7958)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7450..7469)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8129..8920,8921..8989))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8752..8771
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8990..9094)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9120..9575)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9257..9278)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9469..9488
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9702..9724
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9716..9733
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9717..9733
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9740..9759
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9740..9758
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc
      541 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcct cgagaattct
     1741 cacgcgtctg caggatatca tgacatactt ccctgaggag gtggtcgaac atattttctc
     1801 attccttccc gcccaacgag ataggaatac cgtgtccctg gtgtgcaagg tctggtatga
     1861 gatcgaacgc ttgagccgca gaggtgtttt tgtgggtaat tgttatgccg tacgagccgg
     1921 gcgagtggcc gcccggttcc cgaacgtcag ggcccttaca gtgaagggca agcctcactt
     1981 tgccgacttc aatttggtgc ctccggactg gggcggctac gccgggccct ggatcgaggc
     2041 cgctgcgcgc gggtgtcatg gtctggaaga actcaggatg aaaaggatgg tggtgtcaga
     2101 cgagtcactg gaactgcttg cccgcagctt cccccgattc cgcgccctgg tgctgatcag
     2161 ctgtgaagga tttagcaccg acggtctggc tgctgtagct agccattgta aactgctgcg
     2221 agaactggac ctgcaggaaa atgaggtaga ggatagggga cctcggtggc tgagctgttt
     2281 cccggatagc tgcactagcc tggtgagctt gaatttcgct tgtatcaagg gcgaagtgaa
     2341 tgccgggagt ttggagagac tcgtttcccg atcccccaac ctgagatctc tgcggctgaa
     2401 cagatccgtt tctgtggaca cgctcgccaa gattttgctc cggactccca acctggagga
     2461 tctggggacc ggaaatctga ccgatgattt ccagacagag agctacttta agctcactag
     2521 tgctctggaa aaatgcaaaa tgcttaggtc actgagcggg ttttgggacg ctagccctgt
     2581 ctgcctgtct tttatctatc ccctgtgtgc acagttgacc ggacttaatc tgagttacgc
     2641 tccaaccctc gacgctagtg accttactaa gatgatcagc cgctgcgtga agctccaaag
     2701 gctctgggta ctggattgta tttcagacaa gggactgcaa gttgtggcct cctcctgcaa
     2761 agatctgcag gagctgaggg tgttcccttc agatttctat gtggccggat acagcgcagt
     2821 caccgaggag ggattggtag cagtgtccct ggggtgcccc aaactcaata gtttgctgta
     2881 cttctgtcat cagatgacca atgctgccct tgttaccgtt gccaaaaact gtccaaactt
     2941 cacaaggttt agactgtgca tcttggagcc cggtaagccc gacgtcgtaa cgtcacagcc
     3001 actggacgaa gggttcggtg ccatagtacg cgaatgtaag ggtctccagc ggctgagcat
     3061 atccggcctg ctgactgaca aagtgttcat gtatattggg aagtacgcca agcagctgga
     3121 aatgctgtca atcgcattcg ccggagattc agataaaggg atgatgcacg tgatgaacgg
     3181 gtgcaagaac ctgagaaaac tcgaaattcg cgattcccct tttggggacg ccgcactgct
     3241 gggcaatttc gcccggtacg agacgatgag gtcactttgg atgtcaagct gcaacgtgac
     3301 cctcaaggga tgccaagtcc tggctagtaa aatgccaatg ctgaatgttg aggtcatcaa
     3361 cgaacgcgac ggtagtaatg aaatggagga aaaccacggc gatctgccca aagtggagaa
     3421 actgtacgtc tatcggacca cagctggcgc acgagatgac gctcccaatt tcgtgaaaat
     3481 tctgggcaag cccatcccca accccctgct gggcctggac agcacctgac ccgggtaagc
     3541 ggcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
     3601 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     3661 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     3721 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     3781 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     3841 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     3901 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     3961 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     4021 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca
     4081 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     4141 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     4201 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     4261 cagcggatca attccgaagt tcctattctc tagaaagtat aggaacttca ggtctgaaga
     4321 ggagtttacg tccagccaag ctagcttggc tgcaggtcgt cgaaattcta ccgggtaggg
     4381 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     4441 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     4501 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     4561 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     4621 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     4681 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     4741 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
     4801 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     4861 ccgggccttt cgacctgcag cctgttgaca attaatcatc ggcatagtat atcggcatag
     4921 tataatacga caaggtgagg aactaaacca tgggatcggc cattgaacaa gatggattgc
     4981 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     5041 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     5101 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     5161 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     5221 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     5281 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     5341 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     5401 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     5461 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc gtcgtgaccc
     5521 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     5581 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     5641 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     5701 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaggggatc
     5761 aattctctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     5821 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     5881 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     5941 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6001 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgact agagcttgcg
     6061 gaacccttcg aagttcctat tctctagaaa gtataggaac ttcatcagtc aggtacataa
     6121 tggatccgga agagggggaa gggattctcc caggcccagg gcggtcctca gaagccagga
     6181 ggcagcagag aactcccaga aaggtattgc aacactcccc tcccccctcc ggagaagggt
     6241 gcggccttct ccccgcctac tccactgcag ctcccttact gataacaact cagagcgact
     6301 ttgggagagc aagtgcttcc tgcctccaaa acagcccaac tgagccctcg ctccttccct
     6361 ccactccccg gagtgcgcga tggaggtctg gctcagcacg cccctcttga ggcaactcaa
     6421 gtcggaaacg tgcttgcacc cgccccgcag ccgctcagcc ctactgcccg tccccgcccc
     6481 cagcgcgcgc ttcctgccac gttgcgcagg ggcgcgcggc cagactctgc ggcgcggggc
     6541 cgaggggagg gccggaacct gggagcgcct cctcgccgcc cccgctggcc ggcggatgga
     6601 ctcaacttgc acgaacacga gccaatggca agggccagtt ttctgggccc cgagagccaa
     6661 tcagacgacg aggcccggcc ggcggcgggt aaaacgactc ccccagagga aggggagggt
     6721 gggcggccgc tcgcgcgcga gctactttcg ctgaccctcc ctcccctccc ccgccccgcc
     6781 agaggccgac cgcgcccgca cgtccagctc gcctcacccc acctacctcc cgccccaccc
     6841 agtgggcaga gcgaggctgc cggcggctgc gcactccggc tgccgttaac tggcggccgc
     6901 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt
     6961 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7021 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7081 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7141 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7201 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7261 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7321 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7381 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7441 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7501 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7561 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7621 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7681 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7741 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7801 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7861 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7921 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7981 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8041 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8101 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8161 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8221 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8281 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8341 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8401 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8461 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8521 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8581 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8641 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8701 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8761 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8821 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8881 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8941 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9001 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9061 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
     9121 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     9181 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     9241 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     9301 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     9361 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     9421 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     9481 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     9541 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa
     9601 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     9661 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     9721 aacgacggcc agtgaattgt aatacgactc actatagggc gaattgggta ccataaatgg
     9781 agaaaaagga gagaggcatt catgggagtg gaaagttaag ccttaggttg gattctcaat
     9841 acatctattg ttgtaaaact ccctggactg agaataggcc caaatgtgga acaccacctg
     9901 acgggagagg tgatagacac tgaaaattaa ggatcaaggc aaaggatcaa caaaaagtgt
     9961 actaaggagt tataaaagaa ctgcagtgtc gaggccccag ctacagcctc gatttgtggt
    10021 gtatgtaact aatctgtctg gtttcatgag tcatcagact tctaagatca ggaaagggaa
    10081 aatgccaatg ctctgtctag gggttggata agccagtcta ataaatgaaa atggggctaa
    10141 aatgagtgtt ctaaaatacc ttttgataag gctgcagaag gagcgggaga aatggatatg
    10201 aagtactggg ctctttaaaa atgattaaaa ttctgcttac atagtctaac tcgcgacact
    10261 gtaatttcat actgtagtaa ggatctcaag caggagagta taaaactcgg gtgagcatgt
    10321 ctttaatcta cctcgatgga aaatactccg aggcggatca caagcaataa taacctgtag
    10381 ttttgctgca taaaacccca gatgactacc tatcctccca ttttccttat ttgcccctat
    10441 taaaaaactt cccgacaaaa ccgaaaatct gtgggaagtc ttgtccctcc aattttacac
    10501 ctgttcaatt cccctgcagg acaacgccca cacaccaggt tagcctttaa gcctctcgag
    10561 gtc
//
