LOCUS       sequence-177758-        6070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6070)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-ILK-N-17
FEATURES             Location/Qualifiers
     source          1..6070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        47..404
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        47..238
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(70..90)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      255..390
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             439..1233
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             439..1233
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(493..512)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1103..1122
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1421..1440)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1465..1512
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1841..2429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2330..2349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2604..2907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2908..3111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3062..3082
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4569..4578
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4575..5276
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(4708..4726)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4976..4995
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5170..5193
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    5401..5522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5438..5457)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5492..5511
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5529..5984)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5666..5687)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5878..5897
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga
       61 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
      121 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
      181 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
      241 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
      301 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
      361 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca
      421 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
      481 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
      541 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
      601 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
      661 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
      721 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
      781 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
      841 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
      901 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
      961 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     1021 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     1081 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     1141 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     1201 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     1261 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     1321 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     1381 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa
     1441 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg
     1501 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     1561 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     1621 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct
     1681 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1741 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1801 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1981 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2101 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2161 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2221 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2281 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2341 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2401 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2461 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2521 ataaccgtat taccgccatg cattagttat taatagtaat caattacggg gtcattagtt
     2581 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     2641 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     2701 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     2761 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     2821 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     2881 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     2941 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     3001 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     3061 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
     3121 aaccgtcaga tccgctagcg ctaccggact cagatctcga gaccgccatg gacgacattt
     3181 tcactcagtg ccgggagggc aacgcagtcg ccgttcgcct gtggctggac aacacggaga
     3241 acgacctcaa ccagggggac gatcatggct tctccccctt gcactgggcc tgccgagagg
     3301 gccgctctgc tgtggttgag atgttgatca tgcggggggc acggatcaat gtaatgaacc
     3361 gtggggatga cacccccctg catctggcag ccagtcatgg acaccgtgat attgtacaga
     3421 agctattgca gtacaaggca gacatcaatg cagtgaatga acatgggaat gtgcccctgc
     3481 actatgcctg tttttggggc caagatcaag tggcagagga cctggtggca aatggggccc
     3541 ttgtcagcat ctgtaacaag tatggagaga tgcctgtgga caaagccaag gcacccctga
     3601 gagagcttct ccgagagcgg gcagagaaga tgggccagaa tctcaaccgt attccataca
     3661 aggacacatt ctggaagggg accacccgca ctcggccccg aaatggaacc ctgaacaaac
     3721 actctggcat tgacttcaaa cagcttaact tcctgacgaa gctcaacgag aatcactctg
     3781 gagagctatg gaagggccgc tggcagggca atgacattgt cgtgaaggtg ctgaaggttc
     3841 gagactggag tacaaggaag agcagggact tcaatgaaga gtgtccccgg ctcaggattt
     3901 tctcgcatcc aaatgtgctc ccagtgctag gtgcctgcca gtctccacct gctcctcatc
     3961 ctactctcat cacacactgg atgccatatg gatccctcta caatgtacta catgaaggca
     4021 ccaatttcgt cgtggaccag agccaggctg tgaagtttgc tttggacatg gcaaggggca
     4081 tggccttcct acacacacta gagcccctca tcccacgaca tgcactcaat agccgtagtg
     4141 taatgattga tgaggacatg actgcccgaa ttagcatggc tgatgtcaag ttctctttcc
     4201 aatgtcctgg tcgcatgtat gcacctgcct gggtagcccc cgaagctctg cagaagaagc
     4261 ctgaagacac aaacagacgc tcagcagaca tgtggagttt tgcagtgctt ctgtgggaac
     4321 tggtgacacg ggaggtaccc tttgctgacc tctccaatat ggagattgga atgaaggtgg
     4381 cattggaagg ccttcggcct accatcccac caggtatttc ccctcatgtg tgtaagctca
     4441 tgaagatctg catgaatgaa gaccctgcaa agcgacccaa atttgacatg attgtgccta
     4501 tccttgagaa gatgcaggac aagggaattc tgcagtcgac ggtaccgcgg gcccgggatc
     4561 caccggtcgc caccatggtg agcaagggcg aggaggtcat caaagagttc atgcgcttca
     4621 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     4681 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     4741 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     4801 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     4861 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     4921 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     4981 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     5041 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     5101 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     5161 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     5221 agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaagtaag
     5281 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     5341 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     5401 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5461 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5521 taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     5581 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     5641 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     5701 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     5761 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     5821 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     5881 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     5941 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     6001 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6061 gctcatgaga
//
