LOCUS       sequence-177766-       10119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split-BioID plasmid: Expresses N-terminally tagged 
            CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3e.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10119)
  AUTHORS   Schopp IM, Amaya Ramirez CC, Debeljak J, Kreibich E, Skribbe M, Wild
            K, Bethune J
  TITLE     Split-BioID a conditional proteomics approach to monitor the 
            composition of spatiotemporally defined protein complexes.
  JOURNAL   Nat Commun. 2017 Jun 6;8:15690. doi: 10.1038/ncomms15690.
  PUBMED    28585547
REFERENCE   2  (bases 1 to 10119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-6"; volume: "8:15690. doi"; pages: " 
            10.1038/ncomms15690"
COMMENT     Sequence Label: pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e
FEATURES             Location/Qualifiers
     source          1..10119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             402..995
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1058..1415
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     1826..1848
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1864..1880
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    1933..1966
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     polyA_signal    2074..2208
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2111..2130)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3991..4020)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(4118..4155)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(4172..4190,4191..4213,4214..4232,4233..4255,4256..4274,
                     4275..4297,4298..4316,4317..4339,4340..4358,4359..4381,
                     4382..4400,4401..4423,4424..4442)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        4457..4495
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4607..4630
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    6357..6404
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(7491..7508)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7491..7507)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7500..7522)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7716..7735)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7835..7857
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7896..7914)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7982..8086
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8087..8155,8156..8947)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8305..8324)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9118..9706
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9607..9626
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9860..9877
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9994..10015
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10030..10035,10036..10053,10054..10060)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10068..10084
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10073..10095
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10092..10108
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10092..10108
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 caagctagct ttgctcttag gagtttccta atacatccca aactcaaata tataaagcat
       61 ttgacttgtt ctatgcccta gggggcgggg ggaagctaag ccagcttttt ttaacattta
      121 aaatgttaat tccattttaa atgcacagat gtttttattt cataagggtt tcaatgtgca
      181 tgaatgctgc aatattcctg ttaccaaagc tagtataaat aaaaatagat aaacgtggaa
      241 attacttaga gtttctgtca ttaacgtttc cttcctcagt tgacaacata aatgcgctgc
      301 tgagcaagcc agtttgcatc tgtcaggatc aatttcccat tatgccagtc atattaatta
      361 ctagtcaatt agttgatttt tatttttgac atatacatgt gaatgaaaga ccccacctgt
      421 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg
      481 agaatagaaa agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac
      541 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct
      601 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      661 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt
      721 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      781 gcttctcgct tctgttcgcg cgcttatgct ccccgagctc aataaaagag cccacaaccc
      841 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
      901 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
      961 agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga
     1021 cccctgccca gggaccaccg acccaccacc gggaggtaag ctggccagca acttatctgt
     1081 gtctgtccga ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag
     1141 ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttcgga acacccggcc
     1201 gcaaccctgg gagacgtccc agggacttcg ggggccgttt ttgtggcccg acctgagtcc
     1261 taaaatcccg atcgtttagg actctttggt gcacccccct tagaggaggg atatgtggtt
     1321 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     1381 tgggaccgaa gccgcgccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
     1441 ctgactgtgt ttctgtattt gtctgaaaat atgggcccgg gctagcctgt taccactccc
     1501 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
     1561 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
     1621 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
     1681 ttttcacctg gcccgcatgg acacccagac caggtggggt acatcgtgac ctgggaagcc
     1741 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
     1801 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc
     1861 tccctttatc cagccctcac tccttctcta ggcgccccca tatggccata tgagatccga
     1921 agttcctatt ccgaagttcc tattcttcaa atagtatagg aacttcagat cataatcagc
     1981 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     2041 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     2101 tacaaataag gcaatagcat cacaaatttc acaaataagg catttttttc actgcattct
     2161 agttttggtt tgtccaaact catcaatgta tcttatcatg tctggatctc aaatccctcg
     2221 gaagctgcgc ctgtcttagg ttggagtgat acatttttat cacttttacc cgtctttgga
     2281 ttaggcagta gctctgacgg ccctcctgtc ttaggttagt gaaaaatgtc actctcttac
     2341 ccgtcattgg ctgtccagct tagctcgcag gggaggtggt ctggatccct aacgcgtctg
     2401 attttcgtct tccacgtcct gcagcgcttc tttattctgc tcttcaccgt caccctgcat
     2461 gtctgaagtc catagtgtca gattatcacg taacaactgc atgataagtg tagagtcctt
     2521 atagctttct tcactcagcg tatccagttc tgcaattgca tcatcaaaag ctgcttttgc
     2581 caacctgcag gcacggtcag gggaattaag aatttcgtag tagaatacgg aaaaattgag
     2641 agcaagacct aagcgaatag gatgcgttgg tggaagttct gtcattgcaa tatcactagc
     2701 agctttataa gccactaggc tgttctccgc agcctccttc ctgtcgtttc ctgtggcaaa
     2761 ttctgccaga tacctgtggt agtccccttt cattttataa tagaaaacct tggactcgcc
     2821 agtgttagct gctggaatga ggtgtttgtc cagtacatcc agaatgtcac aacagattaa
     2881 ctttagctca gtctcaacca tttgccgata ttcccgaatc atttttagct tgtcttctcc
     2941 tcccttgttt tcttctttct gttcaatgct gctgattatt ctccaggagg ctcttctagc
     3001 tccaatcaca ttcttatatg caacagatag gaggtttctt tcttcaactg tcagctccac
     3061 atccatccct gctactttct tcattgaccc caccatttcg tcgtatcgct cagcctgctc
     3121 ggccagcttc gcctggtaca ccagatcctc tcgatcatcc atatcgatgc cccgggaggc
     3181 gtaggagatc tggccgccgc cggaggagcc gccgccatgt ttctcctgct cgaacagctc
     3241 cagagcggct ctcagctctc tgatcagcat ggcggccagg gtgttccggt ccaggttgat
     3301 gccagcctcc tgcagggtga tccagccctg gttcaccacg ctctcctcca ctcttctcat
     3361 ggccatgttg attccggctc cgatcacgat ctgagcggca tcgcctgtct tgcctgtcag
     3421 ctccaccagg atgccagcca gcttgcggtc ctggaggtac aggtcattag gccacttcac
     3481 tctcaccttg tcggctccca gctttctcag cacctcggcc atcacgattc cgatcaccag
     3541 actcagtccg atggcagcag caggtccctg ctccagtctc cagaacatgg acaggtacag
     3601 gttggctccg aatgggctga accacttccg tcctctgcct cctctgccag cctgctggta
     3661 ctcggcgatg caggcgtcgc cggacttcag ctctccgatt ctgtccagca ggtactgatt
     3721 ggtggagtcg atcacaggca gcacggccac gcttccgcca tccagctgtc ccaggatctg
     3781 cttggcgttc agcagctgga taggctcagg caggctgtag cccttgccag gcacggtgaa
     3841 cacgtccact ccccagtctc tcagtgtctg gatgtgcttg ttgatggcgg ctctgctcat
     3901 tcccagggtc tctcccagct gctcgccaga gtggaactcg ccgttggcca gcagggcgat
     3961 cagcttcagg ggcacggtgt tgtccttgtc gagatcctct tctgagatga gtttttgttc
     4021 catctcgacc gcggaggctg gatcggtccc ggtgtcttct atggaggtca aaacagcgtg
     4081 gatggcgtct ccaggcgatc tgacggttca ctaaacgagc tctgcttata taggcctccc
     4141 accgtacacg cctacaagct tctttcactt ttctctatca ctgataggga gtggtaaact
     4201 cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct
     4261 atcactgata gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg
     4321 taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt
     4381 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg
     4441 gagtggtaaa ctcgagggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
     4501 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
     4561 ccgggaccga tccagcctcc gcggaattcg gccggcctca attatggact acaaggacga
     4621 cgatgacaag attaacggac tggctcctta cctgagcaga tgggagaagc tggacaactt
     4681 catcaacaga cctgtgaagc tgatcatcgg cgacaaggaa atcttcggca tctccagagg
     4741 aatcgacaag cagggagctc tgctgctgga gcaggacgga atcatcaagc cctggatggg
     4801 cggagaaatc tccctgagaa gcgcagagaa gctcgaggcg aaccagatct cctacgcctc
     4861 ccggggcggc ggctcctccg gcggcggcgt ttgtttcatg tcctctacgg aactcttctc
     4921 atccacaaga gaggaaggaa gctctggctc aggacccagt tttaggtcta atcaaaggaa
     4981 aatgttaaac ctgctcctgg agagagacac ttcctttacc gtctgtccag atgtccctag
     5041 aactccagtg ggcaaatttc ttggtgattc tgcaaaccta agcattttgt ctggaggaac
     5101 cccaaaatgt tgcctcgatc tttcgaatct tagcagtggg gagataactg ccactcagct
     5161 taccacttct gcagaccttg atgaaactgg tcacctggat tcttcaggac ttcaggaagt
     5221 gcatttagct gggatgaatc atgaccagca cctaatgaaa tgtagcccag cacagcttct
     5281 ttgtagcact ccgaatggtt tggaccgtgg ccatagaaag agagatgcaa tgtgtagttc
     5341 atctgcaaat aaagaaaatg acaatggaaa cttggtggac agtgaaatga aatatttggg
     5401 cagtcccatt actactgttc caaaattgga taaaaatcca aacctaggag aagaccaggc
     5461 agaagagatt tcagatgaat taatggagtt ttccctgaaa gatcaagaag caaaggtgag
     5521 cagaagtggc ctatatcgct ccccgtcgat gccagagaac ttgaacaggc caagactgaa
     5581 gcaggtggaa aaattcaagg acaacacaat accagataaa gttaaaaaaa agtatttttc
     5641 tggccaagga aagctcagga agggcttatg tttaaagaag acagtctctc tgtgtgacat
     5701 tactatcact cagatgctgg aggaagattc taaccagggg cacctgattg gtgatttttc
     5761 caaggtatgt gcgctgccaa ccgtgtcagg gaaacaccaa gatctgaagt atgtcaaccc
     5821 agaaacagtg gctgccttac tgtcggggaa gttccagggt ctgattgaga agttttatgt
     5881 cattgattgt cgctatccat atgagtatct gggaggacac atccagggag ccttaaactt
     5941 atatagtcag gaagaactgt ttaacttctt tctgaagaag cccatcgtcc ctttggacac
     6001 ccagaagaga ataatcatcg tgttccactg tgaattctcc tcagagaggg gcccccgaat
     6061 gtgccgctgt ctgcgtgaag aggacaggtc tctgaaccag tatcctgcat tgtactaccc
     6121 agagctatat atccttaaag gcggctacag agacttcttt ccagaatata tggaactgtg
     6181 tgaaccacag agctactgcc ctatgcatca tcaggaccac aagactgagt tgctgaggtg
     6241 tcgaagccag agcaaagtgc aggaagggga gcggcagctg cgggagcaga ttgcccttct
     6301 ggtgaaggac atgagcccat taattaaata gcgattaata acgaggatct ggccgcgaag
     6361 ttcctattcc gaagttccta ttctctagaa agtataggaa cttcagatcc ggattagtcc
     6421 aatttgttaa agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc
     6481 agctgaagcc tatagagtac gagccataga taaaataaaa gattttattt agtctccaga
     6541 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagctcga cttctgttcg
     6601 cgcgcttctg ctccccgagc tcgagcccac aacccctcac tcggggcgcc agtcctccga
     6661 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
     6721 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
     6781 gtctttcaca catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca
     6841 ttaaatggcc atagtactta aagttacatt ggcttccttg aaataaacat ggagtattca
     6901 gaatgtgtca taaatatttc taattttaag atagtatctc cattggcttt ctactttttc
     6961 ttttattttt ttttgtcctc tgtcttccat ttgttgttgt tgttgtttgt ttgtttgttt
     7021 gttggttggt tggttaattt ttttttaaag atcctacact atagttcaag ctagactatt
     7081 agctactctg taacccaggg tgaccttgaa gtcatgggta gcctgctgtt ttagccttcc
     7141 cacatctaag attacaggta tgagctatca tttttggtat attgattgat tgattgattg
     7201 atgtgtgtgt gtgtgattgt gtttgtgtgt gtgactgtga aaatgtgtgt atgggtgtgt
     7261 gtgaatgtgt gtatgtatgt gtgtgtgtga gtgtgtgtgt gtgtgtgtgc atgtgtgtgt
     7321 gtgtgactgt gtctatgtgt atgactgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     7381 gtgtgtgtgt gttgtgaaaa aatattctat ggtagtgaga gccaacgctc cggctcaggt
     7441 gtcaggttgg tttttgagac agagtctttc acttagcttg gaattaattc actggccgtc
     7501 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7561 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7621 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
     7681 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     7741 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     7801 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     7861 tcaccgtcat caccgaaacg cgcgatgacg aaagggcctc gtgatacgcc tatttttata
     7921 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     7981 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     8041 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     8101 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     8161 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8221 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8281 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     8341 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8401 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8461 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8521 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8581 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8641 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8701 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8761 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8821 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8881 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8941 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     9001 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     9061 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     9121 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     9181 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9241 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     9301 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9361 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9421 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9481 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag
     9541 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9601 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9661 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9721 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9781 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9841 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9901 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     9961 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
    10021 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
    10081 acaatttcac acaggaaaca gctatgacca tgattacgc
//
