LOCUS       sequence-177768-       10074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split-BioID plasmid: Expresses N-terminally tagged 
            CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Schopp IM, Amaya Ramirez CC, Debeljak J, Kreibich E, Skribbe M, Wild
            K, Bethune J
  TITLE     Split-BioID a conditional proteomics approach to monitor the 
            composition of spatiotemporally defined protein complexes.
  JOURNAL   Nat Commun. 2017 Jun 6;8:15690. doi: 10.1038/ncomms15690.
  PUBMED    28585547
REFERENCE   2  (bases 1 to 10074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-6"; volume: "8:15690. doi"; pages: " 
            10.1038/ncomms15690"
COMMENT     Sequence Label: pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFP
FEATURES             Location/Qualifiers
     source          1..10074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             402..995
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1058..1415
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     1826..1848
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1864..1880
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    1933..1966
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     polyA_signal    2074..2208
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2111..2130)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(2401..3111,3112..3114,3115..3117))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2401..3111,3112..3114,3115..3117))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2443..2464)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2792..2811
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3051..3072
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(3946..3975)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(4073..4110)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(4127..4145,4146..4168,4169..4187,4188..4210,4211..4229,
                     4230..4252,4253..4271,4272..4294,4295..4313,4314..4336,
                     4337..4355,4356..4378,4379..4397)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        4412..4450
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4562..4585
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    6312..6359
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(7446..7463)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7446..7462)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7455..7477)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7671..7690)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7790..7812
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7851..7869)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7937..8041
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8042..8110,8111..8902)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8260..8279)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9073..9661
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9562..9581
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9815..9832
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9949..9970
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9985..9990,9991..10008,10009..10015)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10023..10039
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10028..10050
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10047..10063
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10047..10063
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 caagctagct ttgctcttag gagtttccta atacatccca aactcaaata tataaagcat
       61 ttgacttgtt ctatgcccta gggggcgggg ggaagctaag ccagcttttt ttaacattta
      121 aaatgttaat tccattttaa atgcacagat gtttttattt cataagggtt tcaatgtgca
      181 tgaatgctgc aatattcctg ttaccaaagc tagtataaat aaaaatagat aaacgtggaa
      241 attacttaga gtttctgtca ttaacgtttc cttcctcagt tgacaacata aatgcgctgc
      301 tgagcaagcc agtttgcatc tgtcaggatc aatttcccat tatgccagtc atattaatta
      361 ctagtcaatt agttgatttt tatttttgac atatacatgt gaatgaaaga ccccacctgt
      421 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg
      481 agaatagaaa agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac
      541 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct
      601 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      661 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt
      721 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      781 gcttctcgct tctgttcgcg cgcttatgct ccccgagctc aataaaagag cccacaaccc
      841 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
      901 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
      961 agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga
     1021 cccctgccca gggaccaccg acccaccacc gggaggtaag ctggccagca acttatctgt
     1081 gtctgtccga ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag
     1141 ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttcgga acacccggcc
     1201 gcaaccctgg gagacgtccc agggacttcg ggggccgttt ttgtggcccg acctgagtcc
     1261 taaaatcccg atcgtttagg actctttggt gcacccccct tagaggaggg atatgtggtt
     1321 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     1381 tgggaccgaa gccgcgccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
     1441 ctgactgtgt ttctgtattt gtctgaaaat atgggcccgg gctagcctgt taccactccc
     1501 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
     1561 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
     1621 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
     1681 ttttcacctg gcccgcatgg acacccagac caggtggggt acatcgtgac ctgggaagcc
     1741 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
     1801 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc
     1861 tccctttatc cagccctcac tccttctcta ggcgccccca tatggccata tgagatccga
     1921 agttcctatt ccgaagttcc tattcttcaa atagtatagg aacttcagat cataatcagc
     1981 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     2041 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     2101 tacaaataag gcaatagcat cacaaatttc acaaataagg catttttttc actgcattct
     2161 agttttggtt tgtccaaact catcaatgta tcttatcatg tctggatctc aaatccctcg
     2221 gaagctgcgc ctgtcttagg ttggagtgat acatttttat cacttttacc cgtctttgga
     2281 ttaggcagta gctctgacgg ccctcctgtc ttaggttagt gaaaaatgtc actctcttac
     2341 ccgtcattgg ctgtccagct tagctcgcag gggaggtggt ctggatccct aacgcgttgg
     2401 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
     2461 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg
     2521 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
     2581 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
     2641 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
     2701 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
     2761 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
     2821 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
     2881 catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt
     2941 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta
     3001 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
     3061 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatatc
     3121 gatgccccgg gaggcgtagg agatctggcc gccgccggag gagccgccgc catgtttctc
     3181 ctgctcgaac agctccagag cggctctcag ctctctgatc agcatggcgg ccagggtgtt
     3241 ccggtccagg ttgatgccag cctcctgcag ggtgatccag ccctggttca ccacgctctc
     3301 ctccactctt ctcatggcca tgttgattcc ggctccgatc acgatctgag cggcatcgcc
     3361 tgtcttgcct gtcagctcca ccaggatgcc agccagcttg cggtcctgga ggtacaggtc
     3421 attaggccac ttcactctca ccttgtcggc tcccagcttt ctcagcacct cggccatcac
     3481 gattccgatc accagactca gtccgatggc agcagcaggt ccctgctcca gtctccagaa
     3541 catggacagg tacaggttgg ctccgaatgg gctgaaccac ttccgtcctc tgcctcctct
     3601 gccagcctgc tggtactcgg cgatgcaggc gtcgccggac ttcagctctc cgattctgtc
     3661 cagcaggtac tgattggtgg agtcgatcac aggcagcacg gccacgcttc cgccatccag
     3721 ctgtcccagg atctgcttgg cgttcagcag ctggataggc tcaggcaggc tgtagccctt
     3781 gccaggcacg gtgaacacgt ccactcccca gtctctcagt gtctggatgt gcttgttgat
     3841 ggcggctctg ctcattccca gggtctctcc cagctgctcg ccagagtgga actcgccgtt
     3901 ggccagcagg gcgatcagct tcaggggcac ggtgttgtcc ttgtcgagat cctcttctga
     3961 gatgagtttt tgttccatct cgaccgcgga ggctggatcg gtcccggtgt cttctatgga
     4021 ggtcaaaaca gcgtggatgg cgtctccagg cgatctgacg gttcactaaa cgagctctgc
     4081 ttatataggc ctcccaccgt acacgcctac aagcttcttt cacttttctc tatcactgat
     4141 agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga
     4201 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
     4261 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
     4321 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
     4381 tctatcactg atagggagtg gtaaactcga gggtaggcgt gtacggtggg aggcctatat
     4441 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     4501 cctccataga agacaccggg accgatccag cctccgcgga attcggccgg cctcaattat
     4561 ggactacaag gacgacgatg acaagattaa cggactggct ccttacctga gcagatggga
     4621 gaagctggac aacttcatca acagacctgt gaagctgatc atcggcgaca aggaaatctt
     4681 cggcatctcc agaggaatcg acaagcaggg agctctgctg ctggagcagg acggaatcat
     4741 caagccctgg atgggcggag aaatctccct gagaagcgca gagaagctcg aggcgaacca
     4801 gatctcctac gcctcccggg gcggcggctc ctccggcggc ggcgtttgtt tcatgtcctc
     4861 tacggaactc ttctcatcca caagagagga aggaagctct ggctcaggac ccagttttag
     4921 gtctaatcaa aggaaaatgt taaacctgct cctggagaga gacacttcct ttaccgtctg
     4981 tccagatgtc cctagaactc cagtgggcaa atttcttggt gattctgcaa acctaagcat
     5041 tttgtctgga ggaaccccaa aatgttgcct cgatctttcg aatcttagca gtggggagat
     5101 aactgccact cagcttacca cttctgcaga ccttgatgaa actggtcacc tggattcttc
     5161 aggacttcag gaagtgcatt tagctgggat gaatcatgac cagcacctaa tgaaatgtag
     5221 cccagcacag cttctttgta gcactccgaa tggtttggac cgtggccata gaaagagaga
     5281 tgcaatgtgt agttcatctg caaataaaga aaatgacaat ggaaacttgg tggacagtga
     5341 aatgaaatat ttgggcagtc ccattactac tgttccaaaa ttggataaaa atccaaacct
     5401 aggagaagac caggcagaag agatttcaga tgaattaatg gagttttccc tgaaagatca
     5461 agaagcaaag gtgagcagaa gtggcctata tcgctccccg tcgatgccag agaacttgaa
     5521 caggccaaga ctgaagcagg tggaaaaatt caaggacaac acaataccag ataaagttaa
     5581 aaaaaagtat ttttctggcc aaggaaagct caggaagggc ttatgtttaa agaagacagt
     5641 ctctctgtgt gacattacta tcactcagat gctggaggaa gattctaacc aggggcacct
     5701 gattggtgat ttttccaagg tatgtgcgct gccaaccgtg tcagggaaac accaagatct
     5761 gaagtatgtc aacccagaaa cagtggctgc cttactgtcg gggaagttcc agggtctgat
     5821 tgagaagttt tatgtcattg attgtcgcta tccatatgag tatctgggag gacacatcca
     5881 gggagcctta aacttatata gtcaggaaga actgtttaac ttctttctga agaagcccat
     5941 cgtccctttg gacacccaga agagaataat catcgtgttc cactgtgaat tctcctcaga
     6001 gaggggcccc cgaatgtgcc gctgtctgcg tgaagaggac aggtctctga accagtatcc
     6061 tgcattgtac tacccagagc tatatatcct taaaggcggc tacagagact tctttccaga
     6121 atatatggaa ctgtgtgaac cacagagcta ctgccctatg catcatcagg accacaagac
     6181 tgagttgctg aggtgtcgaa gccagagcaa agtgcaggaa ggggagcggc agctgcggga
     6241 gcagattgcc cttctggtga aggacatgag cccattaatt aaatagcgat taataacgag
     6301 gatctggccg cgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttca
     6361 gatccggatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga
     6421 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt
     6481 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     6541 ctcgacttct gttcgcgcgc ttctgctccc cgagctcgag cccacaaccc ctcactcggg
     6601 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
     6661 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
     6721 tacccgtcag cgggggtctt tcacacatgc agcatgtatc aaaattaatt tggttttttt
     6781 tcttaagtat ttacattaaa tggccatagt acttaaagtt acattggctt ccttgaaata
     6841 aacatggagt attcagaatg tgtcataaat atttctaatt ttaagatagt atctccattg
     6901 gctttctact ttttctttta tttttttttg tcctctgtct tccatttgtt gttgttgttg
     6961 tttgtttgtt tgtttgttgg ttggttggtt aatttttttt taaagatcct acactatagt
     7021 tcaagctaga ctattagcta ctctgtaacc cagggtgacc ttgaagtcat gggtagcctg
     7081 ctgttttagc cttcccacat ctaagattac aggtatgagc tatcattttt ggtatattga
     7141 ttgattgatt gattgatgtg tgtgtgtgtg attgtgtttg tgtgtgtgac tgtgaaaatg
     7201 tgtgtatggg tgtgtgtgaa tgtgtgtatg tatgtgtgtg tgtgagtgtg tgtgtgtgtg
     7261 tgtgcatgtg tgtgtgtgtg actgtgtcta tgtgtatgac tgtgtgtgtg tgtgtgtgtg
     7321 tgtgtgtgtg tgtgtgtgtg tgtgtgttgt gaaaaaatat tctatggtag tgagagccaa
     7381 cgctccggct caggtgtcag gttggttttt gagacagagt ctttcactta gcttggaatt
     7441 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     7501 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     7561 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     7621 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     7681 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     7741 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     7801 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga tgacgaaagg gcctcgtgat
     7861 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     7921 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7981 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     8041 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     8101 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     8161 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     8221 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     8281 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     8341 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     8401 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     8461 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     8521 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     8581 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     8641 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     8701 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8761 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     8821 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8881 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8941 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     9001 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     9061 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     9121 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     9181 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     9241 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9301 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     9361 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     9421 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
     9481 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     9541 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     9601 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     9661 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9721 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     9781 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     9841 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     9901 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     9961 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    10021 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgc
//
