LOCUS       sequence-177770-       16293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split-BioID plasmid: Expresses N-terminally tagged 
            CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-Dicer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16293)
  AUTHORS   Schopp IM, Amaya Ramirez CC, Debeljak J, Kreibich E, Skribbe M, Wild
            K, Bethune J
  TITLE     Split-BioID a conditional proteomics approach to monitor the 
            composition of spatiotemporally defined protein complexes.
  JOURNAL   Nat Commun. 2017 Jun 6;8:15690. doi: 10.1038/ncomms15690.
  PUBMED    28585547
REFERENCE   2  (bases 1 to 16293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-6"; volume: "8:15690. doi"; pages: " 
            10.1038/ncomms15690"
COMMENT     Sequence Label: pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer
FEATURES             Location/Qualifiers
     source          1..16293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(229..246)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(229..245)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(238..260)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(454..473)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     573..595
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(634..652)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        720..824
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(825..893,894..1685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1043..1062)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1856..2444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2345..2364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2598..2615
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2732..2753
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2768..2773,2774..2791,2792..2798)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2806..2822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2811..2833
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2830..2846
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2830..2846
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             3259..3852
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    3915..4272
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     4683..4705
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     4721..4737
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    4790..4823
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     polyA_signal    4931..5065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4968..4987)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(11858..11887)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(11985..12022)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(12039..12057,12058..12080,12081..12099,12100..12122,
                     12123..12141,12142..12164,12165..12183,12184..12206,
                     12207..12225,12226..12248,12249..12267,12268..12290,
                     12291..12309)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        12324..12362
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             12474..12497
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      14697..14706
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    15388..15435
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gaatgtgtgt atgtatgtgt gtgtgtgagt gtgtgtgtgt gtgtgtgcat gtgtgtgtgt
       61 gtgactgtgt ctatgtgtat gactgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
      121 gtgtgtgtgt tgtgaaaaaa tattctatgg tagtgagagc caacgctccg gctcaggtgt
      181 caggttggtt tttgagacag agtctttcac ttagcttgga attaattcac tggccgtcgt
      241 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
      301 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      361 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
      421 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      481 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      541 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      601 accgtcatca ccgaaacgcg cgatgacgaa agggcctcgt gatacgccta tttttatagg
      661 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
      721 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      781 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      841 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      901 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
      961 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     1021 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     1081 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1141 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1201 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     1261 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     1321 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     1381 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     1441 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     1501 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     1561 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     1621 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     1681 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     1741 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     1801 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1861 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1921 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1981 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     2041 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2101 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2161 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2221 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
     2281 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2341 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2401 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2461 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2521 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2581 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     2641 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     2701 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     2761 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     2821 aatttcacac aggaaacagc tatgaccatg attacgccaa gctagctttg ctcttaggag
     2881 tttcctaata catcccaaac tcaaatatat aaagcatttg acttgttcta tgccctaggg
     2941 ggcgggggga agctaagcca gcttttttta acatttaaaa tgttaattcc attttaaatg
     3001 cacagatgtt tttatttcat aagggtttca atgtgcatga atgctgcaat attcctgtta
     3061 ccaaagctag tataaataaa aatagataaa cgtggaaatt acttagagtt tctgtcatta
     3121 acgtttcctt cctcagttga caacataaat gcgctgctga gcaagccagt ttgcatctgt
     3181 caggatcaat ttcccattat gccagtcata ttaattacta gtcaattagt tgatttttat
     3241 ttttgacata tacatgtgaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     3301 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagaaaagt tcagatcaag
     3361 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     3421 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
     3481 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     3541 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     3601 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     3661 ttatgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
     3721 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     3781 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     3841 gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc
     3901 caccaccggg aggtaagctg gccagcaact tatctgtgtc tgtccgattg tctagtgtct
     3961 atgactgatt ttatgcgcct gcgtcggtac tagttagcta actagctctg tatctggcgg
     4021 acccgtggtg gaactgacga gttcggaaca cccggccgca accctgggag acgtcccagg
     4081 gacttcgggg gccgtttttg tggcccgacc tgagtcctaa aatcccgatc gtttaggact
     4141 ctttggtgca ccccccttag aggagggata tgtggttctg gtaggagacg agaacctaaa
     4201 acagttcccg cctccgtctg aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg
     4261 tcttgtctgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     4321 tgaaaatatg ggcccgggct agcctgttac cactccctta agtttgacct taggtcactg
     4381 gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga gacgttgggt
     4441 taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag acggcacctt
     4501 taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca
     4561 cccagaccag gtggggtaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg
     4621 ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct
     4681 cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc
     4741 ttctctaggc gcccccatat ggccatatga gatccgaagt tcctattccg aagttcctat
     4801 tcttcaaata gtataggaac ttcagatcat aatcagccat accacatttg tagaggtttt
     4861 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     4921 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaggca atagcatcac
     4981 aaatttcaca aataaggcat ttttttcact gcattctagt tttggtttgt ccaaactcat
     5041 caatgtatct tatcatgtct ggatctcaaa tccctcggaa gctgcgcctg tcttaggttg
     5101 gagtgataca tttttatcac ttttacccgt ctttggatta ggcagtagct ctgacggccc
     5161 tcctgtctta ggttagtgaa aaatgtcact ctcttacccg tcattggctg tccagcttag
     5221 ctcgcagggg aggtggtctg gatccctaac gcgtctaata atagctattg ggaacctgag
     5281 gttgattagc tttgaggctt cggagggctc ttcttgctgc tgcagatttg gcaatcctgt
     5341 aacttcgacc aacaccttta aatttcccct ttcctactac ttccacagtg actctgacct
     5401 tcccgtcgta agttctctca gccgggctaa atttggcagt ttctggttcc atttcaagca
     5461 attctcgcac aggggaacgg ggtacatttg cagaaaactt ttctattagt ggccgcatca
     5521 tgggatagta cacctgccag actgtctcca gtgacatccc actatccatg taaatggcac
     5581 cagcaagcga ctcaaaaata tcccccatgg cctttggaac ttcaatatcc tcttctttct
     5641 cttcatcctc ctcagatctc ctaagctcag aatccattcc ttgcatttca ttcttctcaa
     5701 gctgaaactg cacaaagtca tcaatgacat ggaagagctc aggagagaca gctttgaagt
     5761 acttgtggta gtcgtacttt acagccagcg atgcaaagat ggtgttgttg accagggcag
     5821 accgcaggtc tgtcaggacc cccggggagt gctgccgcgg gtcttcataa aggtgcttgg
     5881 ttatgaggta gtccaaaatc gcatctccca ggaattctaa gcgctggtaa caatcagtga
     5941 tagtattgta gtggtaggag gcatgtgtaa aagcctggag aaggtaagcc ttattcttga
     6001 atctgtagtt gattttcttt tcaaaatttt caaaccccga tataaggtga ttcagtgttt
     6061 tatctgcatc tggatgatca aacatacatc ttggtggaat cttcaaacaa ccatattccg
     6121 agtctttcaa tacagaagag cgtgaactgg ccacagaagc agcagcacag ctcactgaaa
     6181 ggttcttttg ttggctgttg aaattctccc gagtagggca cagggccttt tcccgatcag
     6241 tccttttaat taccgggagc accttcagcc ccagtgaaca gaggaaaagc tgagcagccc
     6301 tctccccaca gctggttaaa tagcagccca gcagggcttc cacacagtcc gctatgcttt
     6361 tgtcagcaat acactgctca gtgtgcaagt cgtaagaaat ggactgcttt cccgtgtcaa
     6421 caccacagtt ttcttctgat ggattccaga accccaccac aaagtcatct tcttcaacag
     6481 ctttgctagg atccagatag cacattgcat cccaagagct gtagtcaaaa tcctcaaaat
     6541 ctgatgaaaa tggcatacta cctaaggagg attttttggg cattttccat tcatatgcag
     6601 aatcagtggt tgaaaaagga gaaagagaga ttttctttac aaaagctcct gaccccatta
     6661 acatattatc tataaatctg atatgttcct gatcatactc caggaaatca tcttcatagt
     6721 cagcctcttc cttcggagcc ctccacatca ggctctcctc ctcctcatcc tcctcctcgt
     6781 aatcctcatc cagtttgcca ttcgccagca tgcagtcttt tgtcatttca tctttttccc
     6841 atttatctgt gttgcttttg tcttgattta ctacataacc aggaggaagc caattcacag
     6901 ggggatcaaa tattgacacc accatgcggc tgggtagtcc cttctttttt ccaaggcgat
     6961 acagattaca gttgctgacc tttttgcttc tcatatatga aaggcggccc tcatgcgcat
     7021 cagggtaagt gcaaaataga tatgtggtga tggcatgctt taaaaaggag tcgccaagca
     7081 tttcaagccg ctccaggtta aatccatcac tagcgtttga cagagtcaaa gcctgaagaa
     7141 taagtccagg attggggcca agagtccttg aggagtaccc aatagaaggg ctctgctcag
     7201 aatccatcct gcccttgagc acttgaatag tgtctgtcgt accaggcatt acggccatca
     7261 caggacttcc atctgaggta gatttgttag catttccatc aaggtattta ttactcagga
     7321 gagtacattc atcgctgggc tggggctggt tctcgtaact gtataaattc tgaatggaat
     7381 atgaggtagt tggttgcacg ggtatttcct gcttgtagta atttagctga tttccttggc
     7441 aaaagtctct gttagctaaa tcataactgc cattggcgag attttgattg taagaaagac
     7501 cattaattgc tgtaagatct gctgaaactt caacgtggag cttaccaggg gactcgctga
     7561 gcaacgttct gcagttcaca gacatttggt catgattttc tagagaggag gttctattag
     7621 caccttgatg tgcagcattt tcagggacaa ttgtgctgtg cttacagtaa ttatcatttt
     7681 cagctgaaga ggagttagaa attgagatga aagatttgct gtcaatagat tttttccacc
     7741 cgaagtctaa gttagggtat ctaaaatccg caggaagtga tctgactccc acgccagcat
     7801 cgctggcagt ctgggctctt agctcctctg cagtcaaaag gcagtgaagg cgataaagta
     7861 tgctggggag acaaacagct tttctccaca gtgatgctgg aattggatgt atagcacaga
     7921 gttctggaac cagtatctgt ttattctgca gactttccca tttggctttc ctcttctcag
     7981 cactgcttaa aggaagcgct ttccccttct gattcaaatg tcgaggtgtc aaaagattaa
     8041 gtcttgaaga tgtgtggtcc acatccagca gtggctggtt gagattggtt aggtcaaggt
     8101 tgtactttgt tttataatat tctgcaaaag tttcatactc aggggaagga aatttactga
     8161 gtggggtaag atcagtgtac acatcagcta cataaaatcg atgaggctga tcaaaattgc
     8221 gatatcttgg aatgataacg gcatcttggt aatcttctaa tttaaaaaca aagggtgttt
     8281 cttttgtata ctttgtactg ggaatgccta tgcgagcttc agacttctca atatcttcca
     8341 tgaatttaaa gtcaatatcc aaagtgctgg agtcattaac aacattaaga ggtagaacac
     8401 agtatgctga atcagcgtct gtaggtttaa attctagtgc aggtttttca agccgaagaa
     8461 tatgtgagaa tatatactgg tgaagtcttg taatcaactc aagcatttgt agagacaaca
     8521 tgaaaccaga cttcttcaac tcaatggata tggtaacctc tccagagcgt gtgtacacag
     8581 gaaagtgtgg aatctgaggt atgggtttgg ccgtcagtat tccaaagcat cttgtggtat
     8641 cttcaggagg atagagcttc cgccttctaa agttgagttc atcaggtaaa ggtgtagtta
     8701 aaaccattcc tatcacatac aggtaacagg gctgatcagg tctgggataa ctatccctca
     8761 aacactctgg aattgctttt gggtagcact gccttcgttt cgtggaacct ggtcttcctg
     8821 gaacactggt ctcttcttca tcatgcaaat caagctcctc ttcatattta acagtctctt
     8881 tcccaactgg catcaaatgg tcatccagtt cgccaatttt gtgcagtttc tcacagcaaa
     8941 tgagagctac aactctttca gccaatcgta cacagctcat tggtggacca acaatggagg
     9001 ctcgaagagg tgagttaatt ggcagataaa gagttgaata aaatgtacca tcaggcaact
     9061 ctcgggttct gcatttagga gctagatgag taaacggatc acttggtaat ctagcacagt
     9121 atctattgat gtgtccaatg gccgtgttga ttgtgactcg tggaccacca tcgtcaggcc
     9181 tcaacacata tggtgggaaa acgtcatcat catccatgac aggatcaatg tcagtctcac
     9241 cagtatcaac cgacttggaa cacttgtttc tcaagatctt ttcaatagct ttgtaggttt
     9301 taaggtcttc ttcaaaactt tttattttgt ctgtatccgc taacattata taattagaga
     9361 tgggtgccct tgctcttcct ttagattgaa cataggatcg atattctgtg ggcaaatcaa
     9421 aacgaaccac caagttgcat tttggtatat caacaccctc ttctacaata cttgttgcaa
     9481 taagcaggtt ggtctcatgt gctcgaaatt tcctaagtac ctcttcctgt tttctgaatt
     9541 ctgcttccat ctgtttgttg cgaggctgat tcttcccaat gccatgtcca gttatgaaat
     9601 tgctactgat ataagccagc tctggatctt gtttgccagc ttcctttatc aatctgttta
     9661 agacaactgc tgtgtatctt ctttccacaa aaataattcc gcacaaaatg ttggtaaaag
     9721 gagaaggaaa atttgtctct ggcttctctt tttcttcaat ttcttcatcc tcatcatcat
     9781 cctcagaatc actccatgac acataattat cctgatttct attattatac cactcaacgc
     9841 tttcaaactg ctgtcgctca tatggtttat atttgcgtaa gatttcgagc agtttgatta
     9901 ctttaggagt tacaaatttc aggtcaagtg aggcaggtga gaagtgctct tcacatagtg
     9961 catgtatttt ccttaggaaa gtgtctgtaa acaataaaaa tttcctgtgc agctcctctt
    10021 gctcatgttt gatgtatttc tgtagttctc ttaccatcat tccagctact ttatctgcac
    10081 accagggtcc cagaactacc aatacggcac gacagtctga tagtatctgt ttcgaaatta
    10141 aagtagaatc tctttctttt gaatgtacag atatattaca atcattgata aaattaagtg
    10201 cttcttctaa ttccatcagc agtctttcat aaagcccact tctgtcagta aatggtccac
    10261 aatccaccac aatctcacat ggctgagaag tatacctgtc taagaccacc aggtcagttg
    10321 cagtttcagc attactctta agaattttct ctagtttctg aatcttttct tccaattcct
    10381 ctggatcaca tttcccattt aaaatggaag cagttagtcc caaaatgcga ggacatgatg
    10441 gacaattttc acagagcttc ataatttctc gataggggtg gtctaggatt gcaagatgac
    10501 actcatcaaa caccaaaagg ttaatgtctg acagtgataa gtaaccattt ttcaaaacat
    10561 tcaaggcgac atagcaagtc ataatgagaa cctggtgctt agtaaactct tggttccatc
    10621 tctcttttgt ccaagatgca tttacttcta ggtttgagta ttccccaacc ttgagatctg
    10681 aatgagttct gacagctgac acttgttgag caacctggtt tgcagagttg accaagaaca
    10741 ccgtcctttt tccatttctg ctgaagtctc ccctgatctg ataggacagc tctttagtga
    10801 gtagtactgc aataaatgtc ttccctgagc cagtgtttaa acagacgatg gtattatgat
    10861 ccagagctgc ttcaagcagt tcaacctgat attttcttgg cgtataaatg ttatcatgaa
    10921 ttgcttcttg ttgccatggc agtccaaaga aaggacccat tggtgaggaa gcaggggtca
    10981 tgagctgcag gcctgccatg ctgaggggtt gcaaagcagg gcttttcatc tcaatgcccc
    11041 gggaggcgta ggagatctgg ccgccgccgg aggagccgcc gccatgtttc tcctgctcga
    11101 acagctccag agcggctctc agctctctga tcagcatggc ggccagggtg ttccggtcca
    11161 ggttgatgcc agcctcctgc agggtgatcc agccctggtt caccacgctc tcctccactc
    11221 ttctcatggc catgttgatt ccggctccga tcacgatctg agcggcatcg cctgtcttgc
    11281 ctgtcagctc caccaggatg ccagccagct tgcggtcctg gaggtacagg tcattaggcc
    11341 acttcactct caccttgtcg gctcccagct ttctcagcac ctcggccatc acgattccga
    11401 tcaccagact cagtccgatg gcagcagcag gtccctgctc cagtctccag aacatggaca
    11461 ggtacaggtt ggctccgaat gggctgaacc acttccgtcc tctgcctcct ctgccagcct
    11521 gctggtactc ggcgatgcag gcgtcgccgg acttcagctc tccgattctg tccagcaggt
    11581 actgattggt ggagtcgatc acaggcagca cggccacgct tccgccatcc agctgtccca
    11641 ggatctgctt ggcgttcagc agctggatag gctcaggcag gctgtagccc ttgccaggca
    11701 cggtgaacac gtccactccc cagtctctca gtgtctggat gtgcttgttg atggcggctc
    11761 tgctcattcc cagggtctct cccagctgct cgccagagtg gaactcgccg ttggccagca
    11821 gggcgatcag cttcaggggc acggtgttgt ccttgtcgag atcctcttct gagatgagtt
    11881 tttgttccat ctcgaccgcg gaggctggat cggtcccggt gtcttctatg gaggtcaaaa
    11941 cagcgtggat ggcgtctcca ggcgatctga cggttcacta aacgagctct gcttatatag
    12001 gcctcccacc gtacacgcct acaagcttct ttcacttttc tctatcactg atagggagtg
    12061 gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact
    12121 tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag
    12181 ggagtggtaa actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact
    12241 ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac
    12301 tgatagggag tggtaaactc gagggtaggc gtgtacggtg ggaggcctat ataagcagag
    12361 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
    12421 gaagacaccg ggaccgatcc agcctccgcg gaattcggcc ggcctcaatt atggactaca
    12481 aggacgacga tgacaagatt aacggactgg ctccttacct gagcagatgg gagaagctgg
    12541 acaacttcat caacagacct gtgaagctga tcatcggcga caaggaaatc ttcggcatct
    12601 ccagaggaat cgacaagcag ggagctctgc tgctggagca ggacggaatc atcaagccct
    12661 ggatgggcgg agaaatctcc ctgagaagcg cagagaagct cgaggcgaac cagatctcct
    12721 acgcctcccg gggcggcggc tcctccggcg gcggcgtttg tttaaacatg atgtactcgg
    12781 gagccggccc cgcacttgca cctcctgcgc cgccgccccc catccaagga tatgccttca
    12841 agcctccacc tagacccgac tttgggacct ccgggagaac aatcaaatta caggccaatt
    12901 tcttcgaaat ggacatcccc aaaattgaca tctatcatta tgaattggat atcaagccag
    12961 agaagtgccc gaggagagtt aacagggaaa tcgtggaaca catggtccag cactttaaaa
    13021 cacagatctt tggggatcgg aagcccgtgt ttgacggcag gaagaatcta tacacagcca
    13081 tgccccttcc gattgggagg gacaaggtgg agctggaggt cacgctgcca ggagaaggca
    13141 aggatcgcat cttcaaggtg tccatcaagt gggtgtcctg cgtgagcttg caggcgttac
    13201 acgatgcact ttcagggcgg ctgcccagcg tcccttttga gacgatccag gccctggacg
    13261 tggtcatgag gcacttgcca tccatgaggt acacccccgt gggccgctcc ttcttcaccg
    13321 cgtccgaagg ctgctctaac cctcttggcg ggggccgaga agtgtggttt ggcttccatc
    13381 agtccgtccg gccttctctc tggaaaatga tgctgaatat tgatgtgtca gcaacagcgt
    13441 tttacaaggc acagccagta atcgagtttg tttgtgaagt tttggatttt aaaagtattg
    13501 aagaacaaca aaaacctctg acagattccc aaagggtaaa gtttaccaaa gaaattaaag
    13561 gtctaaaggt ggagataacg cactgtgggc agatgaagag gaagtaccgc gtctgcaatg
    13621 tgacccggcg gcccgccagt caccaaacat tcccgctgca gcaggagagc gggcagacgg
    13681 tggagtgcac ggtggcccag tatttcaagg acaggcacaa gttggttctg cgctaccccc
    13741 acctcccatg tttacaagtc ggacaggagc agaaacacac ctaccttccc ctggaggtct
    13801 gtaacattgt ggcaggacaa agatgtatta aaaaattaac ggacaatcag acctcaacca
    13861 tgatcagagc gactgctagg tcggcgcccg atcggcaaga agagattagc aaattgatgc
    13921 gaagtgcaag tttcaacaca gatccatacg tccgtgaatt tggaatcatg gtcaaagatg
    13981 agatgacaga cgtgactggg cgggtgctgc agccgccctc catcctctac gggggcagga
    14041 ataaagctat tgcgacccct gtccagggcg tctgggacat gcggaacaag cagttccaca
    14101 cgggcatcga gatcaaggtg tgggccattg cgtgcttcgc cccccagcgc cagtgcacgg
    14161 aagtccatct gaagtccttc acagagcagc tcagaaagat ctcgagagac gccggcatgc
    14221 ccatccaggg ccagccgtgc ttctgcaaat acgcgcaggg ggcggacagc gtggagccca
    14281 tgttccggca cctgaagaac acgtatgcgg gcctgcagct ggtggtggtc atcctgcccg
    14341 gcaagacgcc cgtgtacgcc gaggtcaagc gcgtgggaga cacggtgctg gggatggcca
    14401 cgcagtgcgt gcagatgaag aacgtgcaga ggaccacgcc acagaccctg tccaacctct
    14461 gcctgaagat caacgtcaag ctgggaggcg tgaacaacat cctgctgccc cagggcaggc
    14521 cgccggtgtt ccagcagccc gtcatctttc tgggagcaga cgtcactcac ccccccgccg
    14581 gggatgggaa gaagccctcc attgccgccg tggtgggcag catggacgcc caccccaatc
    14641 gctactgcgc caccgtgcgc gtgcagcagc accggcagga gatcatacaa gacctggccg
    14701 ccatggtccg cgagctcctc atccagttct acaagtccac gcgcttcaag cccacccgca
    14761 tcatcttcta ccgcgacggt gtctctgaag gccagttcca gcaggttctc caccacgagt
    14821 tgctggccat ccgtgaggcc tgtatcaagc tagaaaaaga ctaccagccc gggatcacct
    14881 tcatcgtggt gcagaagagg caccacaccc ggctcttctg cactgacaag aacgagcggg
    14941 ttgggaaaag tggaaacatt ccagcaggca cgactgtgga cacgaaaatc acccacccca
    15001 ccgagttcga cttctacctg tgtagtcacg ctggcatcca ggggacaagc aggccttcgc
    15061 actatcacgt cctctgggac gacaatcgtt tctcctctga tgagctgcag atcctaacct
    15121 accagctgtg tcacacctac gtgcgctgca cacgctccgt gtccatccca gcgccagcat
    15181 actacgctca cctggtggcc ttccgggcca ggtaccacct ggtggataag gaacatgaca
    15241 gtgctgaagg aagccatacc tctgggcaga gtaacgggcg agaccaccaa gcactggcca
    15301 aggcggtcca ggttcaccaa gacactctgc gcaccatgta ctttgcttga ttaattaaat
    15361 agcgattaat aacgaggatc tggccgcgaa gttcctattc cgaagttcct attctctaga
    15421 aagtatagga acttcagatc cggattagtc caatttgtta aagacaggat atcagtggtc
    15481 caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta cgagccatag
    15541 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt
    15601 aggtttggca agctagctcg acttctgttc gcgcgcttct gctccccgag ctcgagccca
    15661 caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat
    15721 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct
    15781 cctctgagtg attgactacc cgtcagcggg ggtctttcac acatgcagca tgtatcaaaa
    15841 ttaatttggt tttttttctt aagtatttac attaaatggc catagtactt aaagttacat
    15901 tggcttcctt gaaataaaca tggagtattc agaatgtgtc ataaatattt ctaattttaa
    15961 gatagtatct ccattggctt tctacttttt cttttatttt tttttgtcct ctgtcttcca
    16021 tttgttgttg ttgttgtttg tttgtttgtt tgttggttgg ttggttaatt tttttttaaa
    16081 gatcctacac tatagttcaa gctagactat tagctactct gtaacccagg gtgaccttga
    16141 agtcatgggt agcctgctgt tttagccttc ccacatctaa gattacaggt atgagctatc
    16201 atttttggta tattgattga ttgattgatt gatgtgtgtg tgtgtgattg tgtttgtgtg
    16261 tgtgactgtg aaaatgtgtg tatgggtgtg tgt
//
