LOCUS       sequence-177771-       15599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split-BioID plasmid: Expresses N-terminally tagged 
            CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15599)
  AUTHORS   Schopp IM, Amaya Ramirez CC, Debeljak J, Kreibich E, Skribbe M, Wild
            K, Bethune J
  TITLE     Split-BioID a conditional proteomics approach to monitor the 
            composition of spatiotemporally defined protein complexes.
  JOURNAL   Nat Commun. 2017 Jun 6;8:15690. doi: 10.1038/ncomms15690.
  PUBMED    28585547
REFERENCE   2  (bases 1 to 15599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-6"; volume: "8:15690. doi"; pages: " 
            10.1038/ncomms15690"
COMMENT     Sequence Label: pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C
FEATURES             Location/Qualifiers
     source          1..15599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(229..246)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(229..245)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(238..260)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(454..473)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     573..595
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(634..652)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        720..824
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(825..893,894..1685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1043..1062)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1856..2444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2345..2364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2598..2615
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2732..2753
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2768..2773,2774..2791,2792..2798)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2806..2822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2811..2833
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2830..2846
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2830..2846
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             3259..3852
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    3915..4272
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     4683..4705
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     4721..4737
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    4790..4823
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     polyA_signal    4931..5065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4968..4987)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8039..8056)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(11164..11193)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(11291..11328)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(11345..11363,11364..11386,11387..11405,11406..11428,
                     11429..11447,11448..11470,11471..11489,11490..11512,
                     11513..11531,11532..11554,11555..11573,11574..11596,
                     11597..11615)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        11630..11668
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             11780..11803
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      14003..14012
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    14694..14741
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gaatgtgtgt atgtatgtgt gtgtgtgagt gtgtgtgtgt gtgtgtgcat gtgtgtgtgt
       61 gtgactgtgt ctatgtgtat gactgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
      121 gtgtgtgtgt tgtgaaaaaa tattctatgg tagtgagagc caacgctccg gctcaggtgt
      181 caggttggtt tttgagacag agtctttcac ttagcttgga attaattcac tggccgtcgt
      241 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
      301 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      361 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
      421 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      481 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      541 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      601 accgtcatca ccgaaacgcg cgatgacgaa agggcctcgt gatacgccta tttttatagg
      661 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
      721 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      781 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      841 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      901 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
      961 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     1021 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     1081 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1141 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1201 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     1261 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     1321 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     1381 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     1441 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     1501 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     1561 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     1621 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     1681 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     1741 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     1801 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1861 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1921 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1981 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     2041 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2101 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2161 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2221 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
     2281 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2341 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2401 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2461 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2521 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2581 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     2641 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     2701 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     2761 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     2821 aatttcacac aggaaacagc tatgaccatg attacgccaa gctagctttg ctcttaggag
     2881 tttcctaata catcccaaac tcaaatatat aaagcatttg acttgttcta tgccctaggg
     2941 ggcgggggga agctaagcca gcttttttta acatttaaaa tgttaattcc attttaaatg
     3001 cacagatgtt tttatttcat aagggtttca atgtgcatga atgctgcaat attcctgtta
     3061 ccaaagctag tataaataaa aatagataaa cgtggaaatt acttagagtt tctgtcatta
     3121 acgtttcctt cctcagttga caacataaat gcgctgctga gcaagccagt ttgcatctgt
     3181 caggatcaat ttcccattat gccagtcata ttaattacta gtcaattagt tgatttttat
     3241 ttttgacata tacatgtgaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     3301 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagaaaagt tcagatcaag
     3361 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     3421 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
     3481 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     3541 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     3601 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     3661 ttatgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
     3721 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     3781 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     3841 gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc
     3901 caccaccggg aggtaagctg gccagcaact tatctgtgtc tgtccgattg tctagtgtct
     3961 atgactgatt ttatgcgcct gcgtcggtac tagttagcta actagctctg tatctggcgg
     4021 acccgtggtg gaactgacga gttcggaaca cccggccgca accctgggag acgtcccagg
     4081 gacttcgggg gccgtttttg tggcccgacc tgagtcctaa aatcccgatc gtttaggact
     4141 ctttggtgca ccccccttag aggagggata tgtggttctg gtaggagacg agaacctaaa
     4201 acagttcccg cctccgtctg aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg
     4261 tcttgtctgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     4321 tgaaaatatg ggcccgggct agcctgttac cactccctta agtttgacct taggtcactg
     4381 gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga gacgttgggt
     4441 taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag acggcacctt
     4501 taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca
     4561 cccagaccag gtggggtaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg
     4621 ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct
     4681 cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc
     4741 ttctctaggc gcccccatat ggccatatga gatccgaagt tcctattccg aagttcctat
     4801 tcttcaaata gtataggaac ttcagatcat aatcagccat accacatttg tagaggtttt
     4861 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     4921 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaggca atagcatcac
     4981 aaatttcaca aataaggcat ttttttcact gcattctagt tttggtttgt ccaaactcat
     5041 caatgtatct tatcatgtct ggatctcaaa tccctcggaa gctgcgcctg tcttaggttg
     5101 gagtgataca tttttatcac ttttacccgt ctttggatta ggcagtagct ctgacggccc
     5161 tcctgtctta ggttagtgaa aaatgtcact ctcttacccg tcattggctg tccagcttag
     5221 ctcgcagggg aggtggtctg gatccctaac gcgtctaata atttacaggg actccccgct
     5281 gagcaggtcc ccaggcagca gggtggttag cggcgtgggg ctgcctatca ccctgctgtc
     5341 gtcggcgctg ggcgggcccc acaggctgct ggagtactgg ggcaccccgc cccacagcag
     5401 ctcactgctc cccttgccac ccaggcacgg ggcgttccag tggccagcgt cgctgcgtac
     5461 caggccatgg gagctgcccg ctgcgctgag ccgcggctgg ctggacgcgc tgctggactg
     5521 ccagctggaa gtgggtggca gcgcctggcc ttgggctaag aagcgattca cttcttcttc
     5581 accagcgaac tcggccagga tggtagtgtt tcccaggacg cacatgtgca gagacttctg
     5641 ggccttggca gcctcctcct tggagctgta ccggaccaca gcattgcctt gagtcagatt
     5701 caggtggaat gtgataagag gcccatgttg caaacacaat gtccgcagtg tagaaccatc
     5761 aatctgggga gtgaggtttc gaagaacgag ccagctgctg gttcttcctg aggtgtcggt
     5821 gctccaggca gaacccgagg agtaggagct ggtccagccg agggggctgg caccccaggt
     5881 ggaggagggc ttgggattgg ttaaccctgg aggtggcctc gtgggtgcag tactgtttct
     5941 gggcaccttc catagttcat gagacagaga ggcttgcgtg tgggaggtag ggccagagga
     6001 ccacgtcgat ttgatgtctg acagtttacc tccactcttg aggaggtagc ggttgacatc
     6061 ctggatggtg gtgttgatgg taggcccagt ggggacactg ccaggagtga cgtcagggtc
     6121 attctcaggg tcaatattct gcagtccttt ccatggaact cccggatgga attctggggg
     6181 ccagttgatg ctagagccat ttgagatttt atcactgtca gatttggcac gtgaccagct
     6241 atggggaaca gctacgggag gactggctgg tgactcactg ttctggatta aatcgtaccg
     6301 gccataggag tcatcaatgg aggacttacc tggaggccca atgcttagac ctccagggat
     6361 agcaccatgc ttgctagggt tctgctccag gggggaagcg gcactgggca agttatccat
     6421 ggagttgggg tgcgtccact gggggaggcg tgactgggac tgggatgggt ccttcaccga
     6481 caagccaccg gtcatgtcca tgctgttgac attcatgttt gggttcagtc cagcgagagg
     6541 gtaaggagca aaggtgttgg gtgaagactg ctgctctttg gtctgcaggt caggtaggcc
     6601 gggagtctgt gggtgcgagg ggaagctgtc catggccgat ttgcctgcag aggggtgcag
     6661 agacaggtgc ggcgggggcg gtggcggtgg tggctgcttc acgagcaggg cctgggccag
     6721 ctggcgctgg tgctgctgga tctgctgctg cagattagtg attgtgcgcg caacttgctg
     6781 ctcctgttgt ctcatggatc cggacacatt acgctgggcc tgtaacatct gctgctggat
     6841 ttgtaaacgt tggtatgcca gctgcagctg atagagctgg ttcaacatcg tcatatgttg
     6901 aggattaatt ggcgaggtta atagtgcagg gttgagacca atgttttttg ctgcaaactg
     6961 caaaagctgt gcttgaacct gaggggatag aaactgaggc acttgagcac ggagactggg
     7021 ctgggaagag ttaagaggct gcactggtgg ctgtggcggc tgctgcatgg tcctggcttg
     7081 tgctgctcca ctattgccaa acatacccag attgccactc ggtatctgtc tagaagaatt
     7141 caagttttgc atgcccagcc cggaggcaat cccacccagg gcctgactgg ggagtgcact
     7201 gtgtgaaagg gggagcttca ggcttgggga aggcaagaac ggtgaaggcg tgggctcttc
     7261 cacgaggccg ccatccttgt caagaaaggt ggggcggtcc acggaagact ctttggagat
     7321 tggcgggcgg cagccgaggg gcttggcggc cattccatta tggtcggtca ctcccagccc
     7381 acgcttgtcc acgtccacct tcttttccag cagagcgctc atggcctgat caagattcat
     7441 attgttactc ttcaaggcct cctcagctgg ctctctcggg aagcccatgt ctgtgagttg
     7501 tttgatcagc cggctcatga tccaggcctc atcctgcttg ccagacgtct tcatgccctt
     7561 ttgaagtcct tttttggggg cgttccccca ggagctgcag gaggaggtgc tttcttggga
     7621 agcagcatta ttccacacgt ccccttcttc atcctcccac tggctggtac tgaggttcat
     7681 ttcatcccca ccactgcccc agccttcttg catagatttt gaagctggtt tgcacagggc
     7741 tgaggcagca acaggtgcgc cagctcttcc aaatgccacc ggcggctcgg cagggtgatc
     7801 tccccacccg ctgccactgc tgggtggctt cccccatgct gctgtgccat tatccaccag
     7861 ggtagaaggg gaggatggtt ctccccaaga gttttcagtc tttgagtgaa cattaggcat
     7921 ttctccccag cctgatgaaa ctttcctacc tggaccaagc aacggtgatc gattcagctg
     7981 agtaccagcc tggtgcgggg gcggcgtctc gaccctgtgt gtggtgttgc tcgtggtagt
     8041 ggtggtggtg gtggtggtat tggtcgtggt actgctctgg atgaccgggt tgtttctatc
     8101 ccacatgttt acagttttat tgttatagtt gcttgggtcc ccccaagctg aggtaccatc
     8161 atcaatttcc attttgcggc gaatggacgg tggagagggt tcttcccagc cagttgcttc
     8221 actgctgtcc ttctgtttga ccggtaccgg ccccccgatc caccctgttc ctgtctgttt
     8281 cacagaagca gctcctcccc agttactcac attgttgtct tggggtttgc tagcccagtt
     8341 ctgttggggg ccaggtttta aagtctcccc ccagtttgta cctggattgg cctttgtatt
     8401 tgtgctgttg ccccagccac tgttcccaga tctcgtggtg tcattccaac cagaccctga
     8461 cctattactg tcagcatccc atccagatcc attttttttc ccatccccca agtgtccaag
     8521 gacagacgat gaatctgccc aatctccccc tcctgcactc caggctggat ttgatctttg
     8581 tccatctccc cagtgttggg atttgggctt tggaggctca ccccaggtgg gtgacttgtc
     8641 ctcctgattt gcggccccac tccaagcatg gccactcttg gcagcagcag cagtactaac
     8701 agcagtagag cttatcgagt ccccccaaac ccccgggcca cttggcgctt tgttgttgct
     8761 gtctccccaa cctgtgtttg ctggcacagc ggcaggtggc gcgttgaccc acccagatac
     8821 tgaagaggta tttgtactgt tcatgatgga cccatcgttc ttcccccctg agtttgaagc
     8881 ctgagtagct gcacaacccc aggcctctgt cccattgtca tttttccttt cagacctagg
     8941 ggattcttca aattcccagg cagtgttttg ctttacagga gtctgtcccc aaccagtatt
     9001 agacagaact cttgggtcaa gatcattcct tggcaactgg atgtgccctt ggtctataat
     9061 ccctttatct cgccttcggc cttctcccgt tccttccctc ccagtgcttc cttcattgtg
     9121 gttgccagaa ccatcactac ttccttcact tgctgtagtt ccagaagatg ccgctttggc
     9181 ccaggagttc atgtgttcac caccaggctg tacggtaggg ttctggctgg tgacactagg
     9241 actctcccac cctgttgatc ctttcccatt gatgtcactg ttggaatgct ggtttggggg
     9301 ctttccccat tccccattca caccgttaga ggtgcttcgg ctggggtggc cccaagctcc
     9361 tgaatggata ttaccaacgc cattgttgcc actgcccctt ccccaggcga gtattccagg
     9421 accagcagga ggtcctgaat cccaagcatt tcctccactt ccttcctttt gcacagcact
     9481 gctggatcca ttttgtttag cacttaatgc tgagttcaca gtgtctccat tcccctgact
     9541 gaacccagaa ttgctatttc ctgtggcagg gttacctggg gacagtcccc acacagaatt
     9601 gcttattcct tcagcactgc ccccactgac ttgagaaact gatgatccat tagcaccagg
     9661 aaggccttgc aggtggggcg ggatgatggc ccccatacca acagccatgc cccagtttgg
     9721 cagtccattt gtctgcattg cattgatagg gttgggtgaa gagttcatgg ggttagtgtt
     9781 atttggtcca tcagtgttaa ggttctgagg ttgtgcgctg aaagacacat tctgagaagt
     9841 ggacgtttgt ggttctgtgc tctcttgtgg cagcaagttt ccccaagcac ctaaagtgcc
     9901 tgttgggttc ccgttctggt tgcccatatt ttgacctatg gcactgactg gactgcaaat
     9961 actggaaggg ttgcctgtcg ccacggttcc ttcatgtcca agtacaggcc aggcagctgg
    10021 gttggcatta ggattaaggt taagattaat gccagcattg gagttggaag caccccagca
    10081 attctgactt ctcccatctc caatcatagt gtccattttt ccatccccac cactgacaga
    10141 gcagtgagcc aggccagagc tggagcctgt ggctacaccc cacactctgg ccgcacttcc
    10201 attactgttc gctccccctg ctccaagggc actctgatta gaagggcttt ggacgagtgc
    10261 gccattggtg ccattattgc catttgtctt cttggtatgt ccagtgaagt tgccctgggc
    10321 actccctgta gccatctcaa tgccccggga ggcgtaggag atctggccgc cgccggagga
    10381 gccgccgcca tgtttctcct gctcgaacag ctccagagcg gctctcagct ctctgatcag
    10441 catggcggcc agggtgttcc ggtccaggtt gatgccagcc tcctgcaggg tgatccagcc
    10501 ctggttcacc acgctctcct ccactcttct catggccatg ttgattccgg ctccgatcac
    10561 gatctgagcg gcatcgcctg tcttgcctgt cagctccacc aggatgccag ccagcttgcg
    10621 gtcctggagg tacaggtcat taggccactt cactctcacc ttgtcggctc ccagctttct
    10681 cagcacctcg gccatcacga ttccgatcac cagactcagt ccgatggcag cagcaggtcc
    10741 ctgctccagt ctccagaaca tggacaggta caggttggct ccgaatgggc tgaaccactt
    10801 ccgtcctctg cctcctctgc cagcctgctg gtactcggcg atgcaggcgt cgccggactt
    10861 cagctctccg attctgtcca gcaggtactg attggtggag tcgatcacag gcagcacggc
    10921 cacgcttccg ccatccagct gtcccaggat ctgcttggcg ttcagcagct ggataggctc
    10981 aggcaggctg tagcccttgc caggcacggt gaacacgtcc actccccagt ctctcagtgt
    11041 ctggatgtgc ttgttgatgg cggctctgct cattcccagg gtctctccca gctgctcgcc
    11101 agagtggaac tcgccgttgg ccagcagggc gatcagcttc aggggcacgg tgttgtcctt
    11161 gtcgagatcc tcttctgaga tgagtttttg ttccatctcg accgcggagg ctggatcggt
    11221 cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg atctgacggt
    11281 tcactaaacg agctctgctt atataggcct cccaccgtac acgcctacaa gcttctttca
    11341 cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat
    11401 agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga
    11461 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
    11521 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
    11581 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgagg gtaggcgtgt
    11641 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
    11701 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggaat
    11761 tcggccggcc tcaattatgg actacaagga cgacgatgac aagattaacg gactggctcc
    11821 ttacctgagc agatgggaga agctggacaa cttcatcaac agacctgtga agctgatcat
    11881 cggcgacaag gaaatcttcg gcatctccag aggaatcgac aagcagggag ctctgctgct
    11941 ggagcaggac ggaatcatca agccctggat gggcggagaa atctccctga gaagcgcaga
    12001 gaagctcgag gcgaaccaga tctcctacgc ctcccggggc ggcggctcct ccggcggcgg
    12061 cgtttgttta aacatgatgt actcgggagc cggccccgca cttgcacctc ctgcgccgcc
    12121 gccccccatc caaggatatg ccttcaagcc tccacctaga cccgactttg ggacctccgg
    12181 gagaacaatc aaattacagg ccaatttctt cgaaatggac atccccaaaa ttgacatcta
    12241 tcattatgaa ttggatatca agccagagaa gtgcccgagg agagttaaca gggaaatcgt
    12301 ggaacacatg gtccagcact ttaaaacaca gatctttggg gatcggaagc ccgtgtttga
    12361 cggcaggaag aatctataca cagccatgcc ccttccgatt gggagggaca aggtggagct
    12421 ggaggtcacg ctgccaggag aaggcaagga tcgcatcttc aaggtgtcca tcaagtgggt
    12481 gtcctgcgtg agcttgcagg cgttacacga tgcactttca gggcggctgc ccagcgtccc
    12541 ttttgagacg atccaggccc tggacgtggt catgaggcac ttgccatcca tgaggtacac
    12601 ccccgtgggc cgctccttct tcaccgcgtc cgaaggctgc tctaaccctc ttggcggggg
    12661 ccgagaagtg tggtttggct tccatcagtc cgtccggcct tctctctgga aaatgatgct
    12721 gaatattgat gtgtcagcaa cagcgtttta caaggcacag ccagtaatcg agtttgtttg
    12781 tgaagttttg gattttaaaa gtattgaaga acaacaaaaa cctctgacag attcccaaag
    12841 ggtaaagttt accaaagaaa ttaaaggtct aaaggtggag ataacgcact gtgggcagat
    12901 gaagaggaag taccgcgtct gcaatgtgac ccggcggccc gccagtcacc aaacattccc
    12961 gctgcagcag gagagcgggc agacggtgga gtgcacggtg gcccagtatt tcaaggacag
    13021 gcacaagttg gttctgcgct acccccacct cccatgttta caagtcggac aggagcagaa
    13081 acacacctac cttcccctgg aggtctgtaa cattgtggca ggacaaagat gtattaaaaa
    13141 attaacggac aatcagacct caaccatgat cagagcgact gctaggtcgg cgcccgatcg
    13201 gcaagaagag attagcaaat tgatgcgaag tgcaagtttc aacacagatc catacgtccg
    13261 tgaatttgga atcatggtca aagatgagat gacagacgtg actgggcggg tgctgcagcc
    13321 gccctccatc ctctacgggg gcaggaataa agctattgcg acccctgtcc agggcgtctg
    13381 ggacatgcgg aacaagcagt tccacacggg catcgagatc aaggtgtggg ccattgcgtg
    13441 cttcgccccc cagcgccagt gcacggaagt ccatctgaag tccttcacag agcagctcag
    13501 aaagatctcg agagacgccg gcatgcccat ccagggccag ccgtgcttct gcaaatacgc
    13561 gcagggggcg gacagcgtgg agcccatgtt ccggcacctg aagaacacgt atgcgggcct
    13621 gcagctggtg gtggtcatcc tgcccggcaa gacgcccgtg tacgccgagg tcaagcgcgt
    13681 gggagacacg gtgctgggga tggccacgca gtgcgtgcag atgaagaacg tgcagaggac
    13741 cacgccacag accctgtcca acctctgcct gaagatcaac gtcaagctgg gaggcgtgaa
    13801 caacatcctg ctgccccagg gcaggccgcc ggtgttccag cagcccgtca tctttctggg
    13861 agcagacgtc actcaccccc ccgccgggga tgggaagaag ccctccattg ccgccgtggt
    13921 gggcagcatg gacgcccacc ccaatcgcta ctgcgccacc gtgcgcgtgc agcagcaccg
    13981 gcaggagatc atacaagacc tggccgccat ggtccgcgag ctcctcatcc agttctacaa
    14041 gtccacgcgc ttcaagccca cccgcatcat cttctaccgc gacggtgtct ctgaaggcca
    14101 gttccagcag gttctccacc acgagttgct ggccatccgt gaggcctgta tcaagctaga
    14161 aaaagactac cagcccggga tcaccttcat cgtggtgcag aagaggcacc acacccggct
    14221 cttctgcact gacaagaacg agcgggttgg gaaaagtgga aacattccag caggcacgac
    14281 tgtggacacg aaaatcaccc accccaccga gttcgacttc tacctgtgta gtcacgctgg
    14341 catccagggg acaagcaggc cttcgcacta tcacgtcctc tgggacgaca atcgtttctc
    14401 ctctgatgag ctgcagatcc taacctacca gctgtgtcac acctacgtgc gctgcacacg
    14461 ctccgtgtcc atcccagcgc cagcatacta cgctcacctg gtggccttcc gggccaggta
    14521 ccacctggtg gataaggaac atgacagtgc tgaaggaagc catacctctg ggcagagtaa
    14581 cgggcgagac caccaagcac tggccaaggc ggtccaggtt caccaagaca ctctgcgcac
    14641 catgtacttt gcttgattaa ttaaatagcg attaataacg aggatctggc cgcgaagttc
    14701 ctattccgaa gttcctattc tctagaaagt ataggaactt cagatccgga ttagtccaat
    14761 ttgttaaaga caggatatca gtggtccagg ctctagtttt gactcaacaa tatcaccagc
    14821 tgaagcctat agagtacgag ccatagataa aataaaagat tttatttagt ctccagaaaa
    14881 aggggggaat gaaagacccc acctgtaggt ttggcaagct agctcgactt ctgttcgcgc
    14941 gcttctgctc cccgagctcg agcccacaac ccctcactcg gggcgccagt cctccgattg
    15001 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
    15061 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
    15121 tttcacacat gcagcatgta tcaaaattaa tttggttttt tttcttaagt atttacatta
    15181 aatggccata gtacttaaag ttacattggc ttccttgaaa taaacatgga gtattcagaa
    15241 tgtgtcataa atatttctaa ttttaagata gtatctccat tggctttcta ctttttcttt
    15301 tatttttttt tgtcctctgt cttccatttg ttgttgttgt tgtttgtttg tttgtttgtt
    15361 ggttggttgg ttaatttttt tttaaagatc ctacactata gttcaagcta gactattagc
    15421 tactctgtaa cccagggtga ccttgaagtc atgggtagcc tgctgtttta gccttcccac
    15481 atctaagatt acaggtatga gctatcattt ttggtatatt gattgattga ttgattgatg
    15541 tgtgtgtgtg tgattgtgtt tgtgtgtgtg actgtgaaaa tgtgtgtatg ggtgtgtgt
//
