LOCUS       sequence-177773-       11238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split-BioID plasmid: Expresses N-terminally tagged 
            CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Schopp IM, Amaya Ramirez CC, Debeljak J, Kreibich E, Skribbe M, Wild
            K, Bethune J
  TITLE     Split-BioID a conditional proteomics approach to monitor the 
            composition of spatiotemporally defined protein complexes.
  JOURNAL   Nat Commun. 2017 Jun 6;8:15690. doi: 10.1038/ncomms15690.
  PUBMED    28585547
REFERENCE   2  (bases 1 to 11238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-6"; volume: "8:15690. doi"; pages: " 
            10.1038/ncomms15690"
COMMENT     Sequence Label: pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP
FEATURES             Location/Qualifiers
     source          1..11238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             402..995
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1058..1415
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     1826..1848
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1864..1880
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    1933..1966
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     polyA_signal    2074..2208
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2111..2130)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(2401..3111,3112..3114,3115..3117))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2401..3111,3112..3114,3115..3117))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2443..2464)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2792..2811
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3051..3072
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(3946..3975)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(4073..4110)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(4127..4145,4146..4168,4169..4187,4188..4210,4211..4229,
                     4230..4252,4253..4271,4272..4294,4295..4313,4314..4336,
                     4337..4355,4356..4378,4379..4397)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        4412..4450
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4562..4585
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      6785..6794
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    7476..7523
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(8610..8627)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8610..8626)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8619..8641)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8835..8854)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8954..8976
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9015..9033)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9101..9205
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9206..9274,9275..10066)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9424..9443)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10237..10825
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10726..10745
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10979..10996
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11113..11134
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11149..11154,11155..11172,11173..11179)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11187..11203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11192..11214
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11211..11227
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11211..11227
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 caagctagct ttgctcttag gagtttccta atacatccca aactcaaata tataaagcat
       61 ttgacttgtt ctatgcccta gggggcgggg ggaagctaag ccagcttttt ttaacattta
      121 aaatgttaat tccattttaa atgcacagat gtttttattt cataagggtt tcaatgtgca
      181 tgaatgctgc aatattcctg ttaccaaagc tagtataaat aaaaatagat aaacgtggaa
      241 attacttaga gtttctgtca ttaacgtttc cttcctcagt tgacaacata aatgcgctgc
      301 tgagcaagcc agtttgcatc tgtcaggatc aatttcccat tatgccagtc atattaatta
      361 ctagtcaatt agttgatttt tatttttgac atatacatgt gaatgaaaga ccccacctgt
      421 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg
      481 agaatagaaa agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac
      541 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct
      601 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      661 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt
      721 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      781 gcttctcgct tctgttcgcg cgcttatgct ccccgagctc aataaaagag cccacaaccc
      841 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
      901 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
      961 agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga
     1021 cccctgccca gggaccaccg acccaccacc gggaggtaag ctggccagca acttatctgt
     1081 gtctgtccga ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag
     1141 ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttcgga acacccggcc
     1201 gcaaccctgg gagacgtccc agggacttcg ggggccgttt ttgtggcccg acctgagtcc
     1261 taaaatcccg atcgtttagg actctttggt gcacccccct tagaggaggg atatgtggtt
     1321 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     1381 tgggaccgaa gccgcgccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
     1441 ctgactgtgt ttctgtattt gtctgaaaat atgggcccgg gctagcctgt taccactccc
     1501 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
     1561 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
     1621 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
     1681 ttttcacctg gcccgcatgg acacccagac caggtggggt acatcgtgac ctgggaagcc
     1741 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
     1801 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc
     1861 tccctttatc cagccctcac tccttctcta ggcgccccca tatggccata tgagatccga
     1921 agttcctatt ccgaagttcc tattcttcaa atagtatagg aacttcagat cataatcagc
     1981 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     2041 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     2101 tacaaataag gcaatagcat cacaaatttc acaaataagg catttttttc actgcattct
     2161 agttttggtt tgtccaaact catcaatgta tcttatcatg tctggatctc aaatccctcg
     2221 gaagctgcgc ctgtcttagg ttggagtgat acatttttat cacttttacc cgtctttgga
     2281 ttaggcagta gctctgacgg ccctcctgtc ttaggttagt gaaaaatgtc actctcttac
     2341 ccgtcattgg ctgtccagct tagctcgcag gggaggtggt ctggatccct aacgcgttgg
     2401 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
     2461 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg
     2521 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
     2581 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
     2641 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
     2701 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
     2761 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
     2821 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
     2881 catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt
     2941 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta
     3001 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
     3061 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatatc
     3121 gatgccccgg gaggcgtagg agatctggcc gccgccggag gagccgccgc catgtttctc
     3181 ctgctcgaac agctccagag cggctctcag ctctctgatc agcatggcgg ccagggtgtt
     3241 ccggtccagg ttgatgccag cctcctgcag ggtgatccag ccctggttca ccacgctctc
     3301 ctccactctt ctcatggcca tgttgattcc ggctccgatc acgatctgag cggcatcgcc
     3361 tgtcttgcct gtcagctcca ccaggatgcc agccagcttg cggtcctgga ggtacaggtc
     3421 attaggccac ttcactctca ccttgtcggc tcccagcttt ctcagcacct cggccatcac
     3481 gattccgatc accagactca gtccgatggc agcagcaggt ccctgctcca gtctccagaa
     3541 catggacagg tacaggttgg ctccgaatgg gctgaaccac ttccgtcctc tgcctcctct
     3601 gccagcctgc tggtactcgg cgatgcaggc gtcgccggac ttcagctctc cgattctgtc
     3661 cagcaggtac tgattggtgg agtcgatcac aggcagcacg gccacgcttc cgccatccag
     3721 ctgtcccagg atctgcttgg cgttcagcag ctggataggc tcaggcaggc tgtagccctt
     3781 gccaggcacg gtgaacacgt ccactcccca gtctctcagt gtctggatgt gcttgttgat
     3841 ggcggctctg ctcattccca gggtctctcc cagctgctcg ccagagtgga actcgccgtt
     3901 ggccagcagg gcgatcagct tcaggggcac ggtgttgtcc ttgtcgagat cctcttctga
     3961 gatgagtttt tgttccatct cgaccgcgga ggctggatcg gtcccggtgt cttctatgga
     4021 ggtcaaaaca gcgtggatgg cgtctccagg cgatctgacg gttcactaaa cgagctctgc
     4081 ttatataggc ctcccaccgt acacgcctac aagcttcttt cacttttctc tatcactgat
     4141 agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga
     4201 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
     4261 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
     4321 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
     4381 tctatcactg atagggagtg gtaaactcga gggtaggcgt gtacggtggg aggcctatat
     4441 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     4501 cctccataga agacaccggg accgatccag cctccgcgga attcggccgg cctcaattat
     4561 ggactacaag gacgacgatg acaagattaa cggactggct ccttacctga gcagatggga
     4621 gaagctggac aacttcatca acagacctgt gaagctgatc atcggcgaca aggaaatctt
     4681 cggcatctcc agaggaatcg acaagcaggg agctctgctg ctggagcagg acggaatcat
     4741 caagccctgg atgggcggag aaatctccct gagaagcgca gagaagctcg aggcgaacca
     4801 gatctcctac gcctcccggg gcggcggctc ctccggcggc ggcgtttgtt taaacatgat
     4861 gtactcggga gccggccccg cacttgcacc tcctgcgccg ccgcccccca tccaaggata
     4921 tgccttcaag cctccaccta gacccgactt tgggacctcc gggagaacaa tcaaattaca
     4981 ggccaatttc ttcgaaatgg acatccccaa aattgacatc tatcattatg aattggatat
     5041 caagccagag aagtgcccga ggagagttaa cagggaaatc gtggaacaca tggtccagca
     5101 ctttaaaaca cagatctttg gggatcggaa gcccgtgttt gacggcagga agaatctata
     5161 cacagccatg ccccttccga ttgggaggga caaggtggag ctggaggtca cgctgccagg
     5221 agaaggcaag gatcgcatct tcaaggtgtc catcaagtgg gtgtcctgcg tgagcttgca
     5281 ggcgttacac gatgcacttt cagggcggct gcccagcgtc ccttttgaga cgatccaggc
     5341 cctggacgtg gtcatgaggc acttgccatc catgaggtac acccccgtgg gccgctcctt
     5401 cttcaccgcg tccgaaggct gctctaaccc tcttggcggg ggccgagaag tgtggtttgg
     5461 cttccatcag tccgtccggc cttctctctg gaaaatgatg ctgaatattg atgtgtcagc
     5521 aacagcgttt tacaaggcac agccagtaat cgagtttgtt tgtgaagttt tggattttaa
     5581 aagtattgaa gaacaacaaa aacctctgac agattcccaa agggtaaagt ttaccaaaga
     5641 aattaaaggt ctaaaggtgg agataacgca ctgtgggcag atgaagagga agtaccgcgt
     5701 ctgcaatgtg acccggcggc ccgccagtca ccaaacattc ccgctgcagc aggagagcgg
     5761 gcagacggtg gagtgcacgg tggcccagta tttcaaggac aggcacaagt tggttctgcg
     5821 ctacccccac ctcccatgtt tacaagtcgg acaggagcag aaacacacct accttcccct
     5881 ggaggtctgt aacattgtgg caggacaaag atgtattaaa aaattaacgg acaatcagac
     5941 ctcaaccatg atcagagcga ctgctaggtc ggcgcccgat cggcaagaag agattagcaa
     6001 attgatgcga agtgcaagtt tcaacacaga tccatacgtc cgtgaatttg gaatcatggt
     6061 caaagatgag atgacagacg tgactgggcg ggtgctgcag ccgccctcca tcctctacgg
     6121 gggcaggaat aaagctattg cgacccctgt ccagggcgtc tgggacatgc ggaacaagca
     6181 gttccacacg ggcatcgaga tcaaggtgtg ggccattgcg tgcttcgccc cccagcgcca
     6241 gtgcacggaa gtccatctga agtccttcac agagcagctc agaaagatct cgagagacgc
     6301 cggcatgccc atccagggcc agccgtgctt ctgcaaatac gcgcaggggg cggacagcgt
     6361 ggagcccatg ttccggcacc tgaagaacac gtatgcgggc ctgcagctgg tggtggtcat
     6421 cctgcccggc aagacgcccg tgtacgccga ggtcaagcgc gtgggagaca cggtgctggg
     6481 gatggccacg cagtgcgtgc agatgaagaa cgtgcagagg accacgccac agaccctgtc
     6541 caacctctgc ctgaagatca acgtcaagct gggaggcgtg aacaacatcc tgctgcccca
     6601 gggcaggccg ccggtgttcc agcagcccgt catctttctg ggagcagacg tcactcaccc
     6661 ccccgccggg gatgggaaga agccctccat tgccgccgtg gtgggcagca tggacgccca
     6721 ccccaatcgc tactgcgcca ccgtgcgcgt gcagcagcac cggcaggaga tcatacaaga
     6781 cctggccgcc atggtccgcg agctcctcat ccagttctac aagtccacgc gcttcaagcc
     6841 cacccgcatc atcttctacc gcgacggtgt ctctgaaggc cagttccagc aggttctcca
     6901 ccacgagttg ctggccatcc gtgaggcctg tatcaagcta gaaaaagact accagcccgg
     6961 gatcaccttc atcgtggtgc agaagaggca ccacacccgg ctcttctgca ctgacaagaa
     7021 cgagcgggtt gggaaaagtg gaaacattcc agcaggcacg actgtggaca cgaaaatcac
     7081 ccaccccacc gagttcgact tctacctgtg tagtcacgct ggcatccagg ggacaagcag
     7141 gccttcgcac tatcacgtcc tctgggacga caatcgtttc tcctctgatg agctgcagat
     7201 cctaacctac cagctgtgtc acacctacgt gcgctgcaca cgctccgtgt ccatcccagc
     7261 gccagcatac tacgctcacc tggtggcctt ccgggccagg taccacctgg tggataagga
     7321 acatgacagt gctgaaggaa gccatacctc tgggcagagt aacgggcgag accaccaagc
     7381 actggccaag gcggtccagg ttcaccaaga cactctgcgc accatgtact ttgcttgatt
     7441 aattaaatag cgattaataa cgaggatctg gccgcgaagt tcctattccg aagttcctat
     7501 tctctagaaa gtataggaac ttcagatccg gattagtcca atttgttaaa gacaggatat
     7561 cagtggtcca ggctctagtt ttgactcaac aatatcacca gctgaagcct atagagtacg
     7621 agccatagat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     7681 ccacctgtag gtttggcaag ctagctcgac ttctgttcgc gcgcttctgc tccccgagct
     7741 cgagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
     7801 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     7861 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacac atgcagcatg
     7921 tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca tagtacttaa
     7981 agttacattg gcttccttga aataaacatg gagtattcag aatgtgtcat aaatatttct
     8041 aattttaaga tagtatctcc attggctttc tactttttct tttatttttt tttgtcctct
     8101 gtcttccatt tgttgttgtt gttgtttgtt tgtttgtttg ttggttggtt ggttaatttt
     8161 tttttaaaga tcctacacta tagttcaagc tagactatta gctactctgt aacccagggt
     8221 gaccttgaag tcatgggtag cctgctgttt tagccttccc acatctaaga ttacaggtat
     8281 gagctatcat ttttggtata ttgattgatt gattgattga tgtgtgtgtg tgtgattgtg
     8341 tttgtgtgtg tgactgtgaa aatgtgtgta tgggtgtgtg tgaatgtgtg tatgtatgtg
     8401 tgtgtgtgag tgtgtgtgtg tgtgtgtgca tgtgtgtgtg tgtgactgtg tctatgtgta
     8461 tgactgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg ttgtgaaaaa
     8521 atattctatg gtagtgagag ccaacgctcc ggctcaggtg tcaggttggt ttttgagaca
     8581 gagtctttca cttagcttgg aattaattca ctggccgtcg ttttacaacg tcgtgactgg
     8641 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     8701 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     8761 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     8821 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     8881 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     8941 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     9001 gcgatgacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     9061 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     9121 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     9181 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     9241 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     9301 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     9361 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     9421 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     9481 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     9541 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     9601 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     9661 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     9721 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     9781 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     9841 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     9901 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     9961 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    10021 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    10081 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    10141 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    10201 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    10261 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    10321 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10381 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10441 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    10501 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    10561 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    10621 gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    10681 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    10741 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    10801 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    10861 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    10921 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    10981 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
    11041 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
    11101 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
    11161 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
    11221 ctatgaccat gattacgc
//
