LOCUS       sequence-177774-        9437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP labelling of filamentous fungi by Agrobacterium tumefaciens 
            mediated transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Crespo-Sempere A, Lopez-Perez M, Martinez-Culebras PV, 
            Gonzalez-Candelas L
  TITLE     Development of a green fluorescent tagged strain of Aspergillus 
            carbonarius to monitor fungal colonization in grapes.
  JOURNAL   Int J Food Microbiol. 2011 Aug 2;148(2):135-40. doi: 
            10.1016/j.ijfoodmicro.2011.05.021. Epub 2011 May 30.
  PUBMED    21663991
REFERENCE   2  (bases 1 to 9437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijfoodmicro.2011.05.021"; journalName: "Int J Food 
            Microbiol"; date: "2011-08-2- 2"; volume: "148"; issue: "2"; pages: 
            "135-40"
COMMENT     Sequence Label: pRFHUE-eGFP
FEATURES             Location/Qualifiers
     source          1..9437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(8..29)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(269..288)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     616..637
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(715..732)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(715..731)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(724..746)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    967..991
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(1117..2265)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2564..3358)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3640..4271
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    4410..4434
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     4772..4791
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     terminator      complement(4860..5427)
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene"
     CDS             complement(5597..6622)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(5597..6622)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6627..6987)
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     promoter        7075..9211
                     /gene="gpdA"
                     /label=gpdA promoter
                     /note="promoter from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     intron          9260..9375
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             join(9400..9402,9403..9405,9406..9437,1..682)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9400..9402,9403..9405,9406..9437,1..679)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
       61 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      121 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
      181 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      241 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      301 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      361 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
      421 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
      481 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      541 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
      601 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
      661 catggacgag ctgtacaagt aaattaaacc ctcagcggta ccgagctcga attcactggc
      721 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
      781 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      841 ccaacagttg cgcagcctga atggcgaatg gcgccgctgt tgcccgtctc actggtgaaa
      901 agaaaaacca ccccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt
      961 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca
     1021 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg
     1081 gagagcagat ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc
     1141 atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc
     1201 gacctgctcg cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt
     1261 ttccagcttg agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc
     1321 cgtcggcgac agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa
     1381 cagcacgacg atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc
     1441 caggacgcgg aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt
     1501 gaagcccatc gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg
     1561 gccattgatc gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc
     1621 gccgataggg gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc
     1681 ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac
     1741 cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg
     1801 ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga
     1861 aagctgcatt tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc
     1921 gctgacctgt tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc
     1981 tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc tcctgttcga gacgacgcga
     2041 acgctccacg gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg
     2101 ctcgatcttg gccgtagctt gctggaccat cgagccgacg gactggaagg tttcgcgggg
     2161 cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc
     2221 gatcagttct tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg
     2281 gattgccccg actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc
     2341 cttggtgtcc agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc
     2401 gtccttctcg tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc
     2461 caaatacttg ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt
     2521 gcgctcctgc ttgtcgccgg catcgttgcg ccacatctag gtactaaaac aattcatcca
     2581 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
     2641 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
     2701 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
     2761 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
     2821 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
     2881 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
     2941 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
     3001 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
     3061 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
     3121 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
     3181 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
     3241 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
     3301 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
     3361 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
     3421 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
     3481 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
     3541 acaattatgg gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc
     3601 tccagtggcg tacgcctggg gggatgggag gcccgcgtta gcgggccggg agggttcgag
     3661 aagggggggc accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa
     3721 aaacaaggtt tataaatatt ggtttaaaag caggttaaaa gacaggttag cggtggccga
     3781 aaaacgggcg gaaacccttg caaatgctgg attttcagcc tgtggacagc ccctcaaatg
     3841 tcaataggtg cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc
     3901 ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg cagggcactt atccccaggc
     3961 ttgtccacat catctgtggg aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg
     4021 ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc
     4081 cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt
     4141 tttccgcgag gtatccacaa cgccggcggc cgcggtgtct cgcacacggc ttcgacggcg
     4201 tttctggcgc gtttgcaggg ccatagacgg ccgccagccc agcggcgagg gcaaccagcc
     4261 cggtgagcgt cggaaaggcg ctcggtcttg ccttgctcgt cggatctgga accctgtggt
     4321 tggcatgaac aaacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga
     4381 ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact
     4441 gatagtttaa actgaaggcg ggaaacgaca atctgatcat gagcggagaa ttaagggagt
     4501 cacgaagcta aatccgttta aacggcgcgc cggccggccg cgatcgcgca ctagtcgggg
     4561 gatcctctag atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     4621 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
     4681 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     4741 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     4801 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcctctaga
     4861 aagaaggatt acctctaaac aagtgtacct gtgcattctg ggtaaacgac tcataggaga
     4921 gttgtaaaaa agtttcggcc ggcgtattgg gtgttacgga gcattcacta ggcaaccatg
     4981 gttactattg tatacccatc ttagtaggaa tgattttcga ggtttatacc tacgatgaat
     5041 gtgtgtcctg taggcttgag agttcaagga agaaacagtg caattatctt tgcgaaccca
     5101 ggggctggtg acggaatttt catagtcaag ctatcagagt aaagaagagg agcatgtcaa
     5161 agtacaatta gagacaaata tatagtcgcg tggagccaag agcggattcc tcagtctcgt
     5221 aggtctcttg acgaccgttg atctgcttga tctcgtctcc cgaaaatgaa aatagactct
     5281 gctaagctat tcttctgctt cgccggagcc tgaagggcgt actagggttg cgaggtccaa
     5341 tgcattaatg cattgcagat gagctgtatc tggaagaggt aaacccgaaa cgcgttttat
     5401 tcttgttgac atggagctat taaatcacta gaaggcactc tttgctgctt ggacaaatga
     5461 acgtatctta tcgagatcct gaacaccatt tgtctcaact ccggagctga catcgacacc
     5521 aacgatctta tatccagatt cgtcaagctg tttgatgatt tcagtaacgt taagtggatc
     5581 ccggtcggca tctactctat tcctttgccc tcggacgagt gctggggcgt cggtttccac
     5641 tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt
     5701 gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc
     5761 aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc
     5821 ggagcatata cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt
     5881 agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct
     5941 cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat
     6001 tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc
     6061 agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt
     6121 cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac
     6181 gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc
     6241 taagatcggc cgcagcgatc gcatccatgg cctccgcgac cggctgcaga acagcgggca
     6301 gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg
     6361 tcaggctctc gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg
     6421 caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca
     6481 ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga
     6541 gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg
     6601 cggtgagttc aggctttttc atatcgatgc ttgggtagaa taggtaagtc agattgaatc
     6661 tgaaataaag ggaggaaggg cgaacttaag aaggtatgac cgggtcgttc acttaccttg
     6721 cttgacaaac gcaccaagtt atcgtgcacc aagcagcaga tgataataat gtcctcgttc
     6781 ctgtctgcta ataagagtca cacttcgagc gccgccgcta ctgctacaag tggggctgat
     6841 ctgaccagtt gcctaaatga accatcttgt caaacgacac aaattttgtg ctcaccgcct
     6901 ggacgactaa accaaaatag gcattcattg ttgacctcca ctagctccag ccaagcccaa
     6961 aaagtgctcc ttcaatatca tcttctgtcg aggcctgatc atcgatgggc ccatcgatga
     7021 tcaggcctcg agatctagag gatccttgcg cgcctaggcg gccgtggcca gcccgaattc
     7081 ccttgatctc tacacacagg ctcaaatcaa taagaagaac ggttcgtctt tttcgtttat
     7141 atcttgcatc gtcccaaagc tattggcggg atattctgtt tgcagttggc tgacttgaag
     7201 taatctctgc agatctttcg acactgaaat acgtcgagcc tgctccgctt ggaagcggcg
     7261 aggagcctcg tcctgtcaca actaccaaca tggagtacga taagggccag ttccgccagc
     7321 tcattaagag ccagttcatg ggcgttggca tgatggccgt catgcatctg tacttcaagt
     7381 acaccaaccc tcttctgatc cagtcgatca tcccgctgaa gggcgctttc gaatcgaatc
     7441 tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
     7501 ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
     7561 ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
     7621 cggagcggtg tcaagaggat attcttcgct ctgtattata gataagatga tgaggaattg
     7681 gaggtagcat agcttcattt ggatttgctt tccaggctga gactctagct tggagcatag
     7741 agggtccctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tcccgtgaac
     7801 cttttattca ccaatgagca ttggaatgaa catgaatctg aggactgcaa tcgccatgag
     7861 gttttcgaaa tacatccgga tgtcgaaggc ttggggcacc tgcgttggtt gaatttagaa
     7921 cgtggcacta ttgatcatcc gatagctctg caaagggcgt tgcacaatgc aagtcaaacg
     7981 ttgctagcag ttccaggtgg aatgttatga tgagcattgt attaaatcag gagatatagc
     8041 atgatctcta gttagctcac cacaaaagtc agacggcgta accaaaagtc acacaacaca
     8101 agctgtaagg atttcggcac ggctacggaa gacggagaag cccaccttca gtggactcga
     8161 gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
     8221 aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
     8281 ataaacttta agcctaaact atacagaata agatggtgga gagcttatac cgagctccca
     8341 aatctgtcca gatcatggtt gaccggtgcc tggatcttcc tatagaatca tccttattcg
     8401 ttgacctagc tgattctgga gtgacccaga gggtcatgac ttgagcctaa aatccgccgc
     8461 ctccaccatt tgtagaaaaa tgtgacgaac tcgtgagctc tgtacagtga ccggtgactc
     8521 tttctggcat gcggagagac ggacggacgc agagagaagg gctgagtaat aagcgccact
     8581 gcgccagaca gctctggcgg ctctgaggtg cagtggatga ttattaatcc gggaccggcc
     8641 gcccctccgc cccgaagtgg aaaggctggt gtgcccctcg ttgaccaaga atctattgca
     8701 tcatcggaga atatggagct tcatcgaatc accggcagta agcgaaggag aatgtgaagc
     8761 caggggtgta tagccgtcgg cgaaatagca tgccattaac ctaggtacag aagtccaatt
     8821 gcttccgatc tggtaaaaga ttcacgagat agtaccttct ccgaagtagg tagagcgagt
     8881 acccggcgcg taagctccct aattggccca tccggcatct gtagggcgtc caaatatcgt
     8941 gcctctcctg ctttgcccgg tgtatgaaac cggaaaggcc gctcaggagc tggccagcgg
     9001 cgcagaccgg gaacacaagc tggcagtcga cccatccggt gctctgcact cgacctgctg
     9061 aggtccctca gtccctggta ggcagctttg ccccgtctgt ccgcccggtg tgtcggcggg
     9121 gttgacaagg tcgttgcgtc agtccaacat ttgttgccat attttcctgc tctccccacc
     9181 agctgctctt ttcttttctc tttcttttcc catcttcagt atattcatct tcccatccaa
     9241 gaacctttat ttcccctaag taagtacttt gctacatcca tactccatcc ttcccatccc
     9301 ttattccttt gaacctttca gttcgagctt tcccacttca tcgcagcttg actaacagct
     9361 accccgcttg agcagacatc acccggggct gaggacttaa tggtgagcaa gggcgaggag
     9421 ctgttcaccg gggtggt
//
