LOCUS       sequence-177786-        5097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration vector at an attB site in Bacteroides; contains the 
            P1T_DP aTC-inducible promoter with the RBS GH023.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Lim B, Zimmermann M, Barry NA, Goodman AL
  TITLE     Engineered Regulatory Systems Modulate Gene Expression of Human 
            Commensals in the Gut.
  JOURNAL   Cell. 2017 Apr 20;169(3):547-558.e15. doi: 
            10.1016/j.cell.2017.03.045.
  PUBMED    28431252
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.03"; journalName: "Cell"; date: "2017-04-20- 
            20"; volume: "169"; issue: "3"; pages: "547-558.e15"
COMMENT     Sequence Label: pNBU2_erm_P1T_DP-GH023
FEATURES             Location/Qualifiers
     source          1..5097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            102..211
                     /note="incP origin of transfer"
     rep_origin      382..770
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    886..904
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    948..966
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(4208..4276,4277..5068)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4426..4445)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caattcgagg gggatcaatt ccgtgatagg tgggctgccc ttcctggttg gcttggtttc
       61 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
      121 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
      181 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
      241 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
      301 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaacggaa ttgatccggc
      361 cacgatgcgt ccggcgtaga ggatctgaag atcagcagtt caacctgttg atagtacgta
      421 ctaagctctc atgtttcacg tactaagctc tcatgtttaa cgtactaagc tctcatgttt
      481 aacgaactaa accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc
      541 atgtttcacg tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta
      601 aatagcctct aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc
      661 ttttaaggtt taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta
      721 gagcctctca aagcaatttt gagtgacaca ggaacactta acggctgaca tgggaattcc
      781 cctccaccgc ggtggctttt tgcacccgct ttccaagaga agaaagcctt gttaaattga
      841 cttagtgtaa aagcgcagta ctgcttgacc ataagaacaa aaaaatctct atcactgata
      901 gggataaagt ttggaagata aagctaaaag ttcttatctt tgcagtctcc ctatcagtga
      961 tagagacgaa ataaagacat ataaaagaaa agacaccatg gtggatcccc cgggctgcag
     1021 gaattcgata tcaagcttat cgataccgtc gactaattgc ctatcttcca gtgatggaac
     1081 agcatttgtg cattggctgc aacaatcagc cttacttgtg cctgttctat ttccgaaccg
     1141 accgcttgta tgaatccatc aaaattcgtt ttctctatgt tggattcctt gttgctcata
     1201 ttgtgatgat aatttctaca aatatagtca ttggtaacta tctatgaaac tgtttgatac
     1261 ttttatagtt gattaaactt gttcatggca tttgccttaa tatcatccgc tatgtcaatg
     1321 tagggtttca tagctttgta gtcgctgtgt cccgtccatt tcatgaccac ctgtgccggg
     1381 attccgagag ccagcgcatt gcagatgaat gtccttcttc ctgcatgggt actgagcaaa
     1441 gcgtatttgg gtgtgacttc atcaatacgt tcatttccct tgtagtaggt ttcccgtaca
     1501 ggctcgttga tttctgccag ttcgcccagc tctttcaggt aatcgttcat cttctggttg
     1561 ctgatgacgg gcagagccat gtaattctcg aaatggatgt ccttgtattt gtccagtatg
     1621 gctttgctgt atttgttcag ttcaatcgtc aggctgtcgg cagtcttgac tgtggttatt
     1681 tcgatgtggt cggacttcac atcgcttctt ttcagattgc gaacatccga ataccgcaaa
     1741 ctcgtaaagc agcagaacag gaaaacatca cgcacacgtt ccaggtattg cttatccttg
     1801 ggtatctggt agtctttcag cttgttcagt tcatcccaag tcaggaagat tacttttttc
     1861 gaggtggttt tcagtttcgg tttgaacgta tcgtatgcaa tgttctgatg atgtcctttc
     1921 ttgaagctcc agcgcaggaa ccatttgagg aatcccattt gcttgccgat ggtgctgttt
     1981 ctcatatcct tggtgtcacg caggaagttg acgtattcgt tcaatccaaa ctcgttgaaa
     2041 tagttgaacg ttgcatcctc cttgaactct ttgaggtggt tcctcactgc tgcaaatttt
     2101 tcataggtgg atgccgtcca gttattctgg ttaccgcact cttttacaaa ctcatcgaac
     2161 acctcccaaa agctgacagg ggcttcttcc ggctgttctt cgctggtgtc tttcattctc
     2221 atgttgaaag cttccttcaa ctgttgggtc gttggcatga cctcctgcac ctcaaattcc
     2281 ttgaaaatat tctggatttc ggcatagtat ttcagcaagt ccgtattgat ttcggctgca
     2341 ctttgcttta gcttgttggt acatccgctc tttacccgct gcttatctgc atcccatttg
     2401 gctacgtcaa tccggtagcc cgttgtaaac tcgatgcgtt ggctggcaaa gatgacacgc
     2461 atacggatgg gtacgttctc tacgattggc acaccgttct ttttccggct ctccaatgca
     2521 aaaatgatgt tgcgcttgat attcataatt gggtgcgttt gaaattctac acccaaatat
     2581 acacccaatt attgagatag caaaagacat ttagaaacat ttacttttac tctatattgt
     2641 aatttacact tgattatcag tcgtttgcag tcttatgata ttctgtgaaa gtataagttc
     2701 gagagcctgt ctctccgcaa aaaacgctga aaatcagcag attgcaaaac aaacaccctg
     2761 ttttacaccc aagaatgtaa agtcggctgt ttttgtttta tttaagataa tacaaccact
     2821 acataataaa agagtagcga tattaaaaga atccgatgag aaaagactaa tatttatcta
     2881 tccattcagt ttgatttttc aggactttac atcgtcctga aagtatttgt tggtaccggt
     2941 accgaggacg cgtaaacatt tacagttgca tgtggcctat tgtttttagc cgttaaatat
     3001 tttataacta ttaaatagcg atacaaattg ttcgaaacta atattgttta tatcatatat
     3061 tctcgcatgt tttaaagctt tattaaattg attttttgta aacagttttt cgtactcttt
     3121 gttaacccat ttcattacaa aagtttcata tttttttctc tctttaaatg ccatttttgc
     3181 tggctttctt tttaatacaa ttaatgtgct atccacttta ggttttggat ggaaataata
     3241 cctaggaatt tttgctaata tagaaatatc tacctctgcc attaacagca atgctagtga
     3301 tctgtttgta tctaataaca ttttagcaaa accatattcc actattaaat aacttattgt
     3361 ggctgaactt tcaaaaacaa tttttcgaat tatatttgtg cttatgttgt aaggtatgct
     3421 gccaaatatt ttatatggat tgtggctagg aaatgtaaat ttcagtatat catcatttac
     3481 tatttgatag ttaggataat ttaagagctt attacgagtt acctcacata atttagaatc
     3541 aatttctatc gccgttacaa aattacatct ctttaccaat ccagcagtaa aatgaccttt
     3601 ccctgcacct atttcaaaga tgttatcttt ttcatctaaa cttatgcaat tcattatttt
     3661 ttctatgtga tattttgaag taataaaatt ttgactatct tttatattta ctttgttcat
     3721 tataacctct ccttaattta ttgcatctct tttcgaatat ttatgttttt tgagaaaaga
     3781 acgtactcat ggttcatccc gatatgcgta tcggtctgta tatcagcaac tttctatgtg
     3841 tttcaactac aatagtcatc tattctcatc tttctgagtc caccccctgc aaagcccctc
     3901 tttacgacat aaaaattcgg tcggaaaagg tatgcaaaag atgtttctct ctttaagaga
     3961 aactcttcgg gatgcaaaaa tatgaaaata actccaattc accaaattat atagcgactt
     4021 ttttacaaaa tgctaaaatt tgttgatttc cgtcaagcaa ttgttgagca aaaatgtctt
     4081 ttacgataaa atgatacctc aatatcaact gtttagcaaa acgatatttc tcttaaagag
     4141 agaaacacct ttttgttcac caatccccga cttttaatcc cgcggccatg attgaaaaag
     4201 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     4261 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     4321 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     4381 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4441 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4501 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4561 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     4621 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     4681 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     4741 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     4801 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     4861 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     4921 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     4981 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5041 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat aacgcgt
//
