LOCUS       sequence-177788-        9877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5 tagged mutant yki in Drosophila..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Oh H, Irvine KD
  TITLE     In vivo analysis of Yorkie phosphorylation sites.
  JOURNAL   Oncogene. 2009 Apr 30;28(17):1916-27. doi: 10.1038/onc.2009.43. Epub
            2009 Mar 30.
  PUBMED    19330023
REFERENCE   2  (bases 1 to 9877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2009.43"; journalName: "Oncogene"; date: "2009-04-30- 
            30"; volume: "28"; issue: "17"; pages: "1916-27"
COMMENT     Sequence Label: pUAST-attB-yki:V5-S168A
FEATURES             Location/Qualifiers
     source          1..9877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..260
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     207..228
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             1644..1685
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1695..1712
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     intron          1802..1867
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1997..2017
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2289..2423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2326..2345)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2380..2399
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    2452..2521
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(2740..2758)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2779..2795)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2779..2795)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2792..2814)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2803..2819
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2827..2833,2834..2851,2852..2857))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2872..2893
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3010..3027)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3181..3769)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3261..3280)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3940..4731,4732..4800))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4563..4582
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4801..4905)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4931..5386)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5068..5089)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5280..5299
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5513..5535
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5527..5544
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5528..5544
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5551..5570
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5551..5569
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5603..5621)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6073..6144,6546..6819,6894..7548,7610..7925,8129..8260,
                     8331..8945)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    9720..9753
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    join(9786..9877,1..3)
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 gagcggagac tctagcgagc gccggagtat aaatagaggc gcttcgtcta cggagcgaca
       61 attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca aataaacaag
      121 cgcagctgaa caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa tcaattaaaa
      181 gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg
      241 aatacaagaa gagaactctg aatagggaat tgggaattcg ttaacagatc tgcggccgcg
      301 gctcgagggt acctactagt ccagtgtggt ggaattctgc agatatgtgc gcgtgcctaa
      361 tcgctaagat aattctatgt agttttcgtt tgtatacaat aagtgccttt tatatgttaa
      421 cgacgatgtc agccagcagc aatacaaaca gcctgatcga gaaggagatc gacgacgagg
      481 acatgctttc gccgatcaag tccaacaacc tggtggtgcg ggtcaaccag gacacggacg
      541 acaacctgca ggcgctattc gacagcgtcc tgaatccggg tgacgccaag cgcccgctgc
      601 agctgcccct gcgcatgcgg aagctgccca actccttctt cacgcccccg gcgccctcgc
      661 actcgcgggc caacagcgcc gactccacct acgacgcggg ctcccagtcg agcatcaaca
      721 tcgggaacaa ggcgtccatc gtccagcagc cagatggcca gtcgcccatc gccgccatcc
      781 cccagctcca gattcagccg tctccccagc acagccgcct ggcgatacat cactcccgag
      841 cccgcgccag ccccgcctcg ctgcagcaga actacaatgt gcgcgcccgg agcgacgcag
      901 cagcagccaa caatccgaat gccaatccga gcagccaaca gcagcccgct gggcccactt
      961 tcccagagaa cagtgcccaa gagttcccca gcggcgcccc ggccagctcg gccattgatc
     1021 tggacgccat gaacacctgc atgtcgcagg acattcccat gtccatgcag acagtgcaca
     1081 agaagcagcg ctcctacgac gtcatcagcc ccattcagtt gaaccgccaa ctaggcgcct
     1141 tgccgccggg atgggagcaa gccaagacca atgatggcca gatctactac ttgaatcata
     1201 ctacaaaatc tacgcagtgg gaggatccca gaatccaata tcgccagcag cagcaaatct
     1261 tgatggccga gcgaataaag cagaatgatg ttttgcaaac tacaaaacaa actaccacat
     1321 cgaccattgc taacaatttg ggtccactgc cggatggttg ggagcaggca gttaccgagt
     1381 ccggagatct ttactttata aatcacattg atcgaacgac ttcatggaat gatcccagaa
     1441 tgcaatctgg gcttagcgtg ctcgactgcc cagataactt agtgtcttcc ctccagattg
     1501 aggataatct ttgcagtaac ttgttcaatg acgcacaggc cattgtaaat ccgccgtctt
     1561 cccacaaacc tgacgatttg gaatggtata aaattaatta tccagcacag tggcggccgc
     1621 tcgagtctag agggcccttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt
     1681 ctacgcgtac cggtcatcat caccatcacc attgagtttc tagaggatct ttgtgaagga
     1741 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     1801 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     1861 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     1921 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     1981 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     2041 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     2101 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattt
     2161 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
     2221 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     2281 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     2341 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     2401 atgtatctta tcatgtctgg atccactagt gtcgacgatg taggtcacgg tctcgaagcc
     2461 gcggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat
     2521 ctggtccatc atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg
     2581 caccgggaag ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg
     2641 tgcgacggcg tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac
     2701 ggcgggcatg tcgacactag ttctagccag cttttgttcc ctttagtgag ggttaatttc
     2761 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     2821 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     2881 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     2941 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     3001 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3061 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3121 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3181 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3241 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3301 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3361 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3421 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3481 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3541 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3601 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3661 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3721 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3781 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3841 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3901 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3961 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4021 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4081 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4141 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4201 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4261 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4321 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4381 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4441 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4501 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4561 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4621 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4681 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4741 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4801 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4861 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4921 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     4981 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     5041 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     5101 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     5161 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     5221 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     5281 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     5341 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     5401 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     5461 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     5521 cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt
     5581 acgtaccggg cccctagtat gtatgtaagt taataaaacc catttttgcg gaaagtagat
     5641 aaaaaaaaca tttttttttt ttactgcact ggatatcatt gaacttatct gatcagtttt
     5701 aaatttactt cgatccaagg gtatttgatg taccaggttc tttcgattac ctctcactca
     5761 aaatgacatt ccactcaaag tcagcgctgt ttgcctcctt ctctgtccac agaaatatcg
     5821 ccgtctcttt cgccgctgcg tccgctatct ctttcgccac cgtttgtagc gttacgtagc
     5881 gtcaatgtcc gccttcagtt gcattttgtc agcggtttcg tgacgaagct ccaagcggtt
     5941 tacgccatca attaaacaca aagtgctgtg ccaaaactcc tctcgcttct tatttttgtt
     6001 tgttttttga gtgattgggg tggtgattgg ttttgggtgg gtaagcaggg gaaagtgtga
     6061 aaaatcccgg caatgggcca agaggatcag gagctattaa ttcgcggagg cagcaaacac
     6121 ccatctgccg agcatctgaa caatgtgagt agtacatgtg catacatctt aagttcactt
     6181 gatctatagg aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac
     6241 aaaaatccca aaccgcaatt gcacaaacaa atagtgacac gaaacagatt attctggtag
     6301 ctgttctcgc tatataagac aatttttgag atcatatcat gatcaagaca tctaaaggca
     6361 ttcattttcg actatattct tttttacaaa aaatataaca accagatatt ttaagctgat
     6421 cctagatgca caaaaaataa ataaaagtat aaacctactt cgtaggatac ttcggggtac
     6481 tttttgttcg gggttagatg agcataacgc ttgtagttga tatttgagat cccctatcat
     6541 tgcagggtga cagcggagcg gcttcgcaga gctgcattaa ccagggcttc gggcaggcca
     6601 aaaactacgg cacgctccgg ccacccagtc cgccggagga ctccggttca gggagcggcc
     6661 aactagccga gaacctcacc tatgcctggc acaatatgga catctttggg gcggtcaatc
     6721 agccgggctc cggatggcgg cagctggtca accggacacg cggactattc tgcaacgagc
     6781 gacacatacc ggcgcccagg aaacatttgc tcaagaacgg tgagtttcta ttcgcagtcg
     6841 gctgatctgt gtgaaatctt aataaagggt ccaattacca atttgaaact cagtttgcgg
     6901 cgtggcctat ccgggcgaac ttttggccgt gatgggcagt tccggtgccg gaaagacgac
     6961 cctgctgaat gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc catccgggat
     7021 gcgactgctc aatggccaac ctgtggacgc caaggagatg caggccaggt gcgcctatgt
     7081 ccagcaggat gacctcttta tcggctccct aacggccagg gaacacctga ttttccaagc
     7141 catggtgcgg atgccacgac atctgaccta tcggcagcga gtggcccgcg tggatcaggt
     7201 gatccaggag ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc ccggcagggt
     7261 gaaaggtctg tccggcggag aaaggaagcg tctggcattc gcctccgagg ctctaaccga
     7321 tccgccgctt ctgatctgcg atgagcccac ctccggactg gactccttta ccgcccacag
     7381 cgtcgtccag gtgctgaaga agctgtcgca gaagggcaag accgtcatcc tgaccattca
     7441 tcagccgtct tccgagctgt ttgagctctt tgacaagatc cttctgatgg ccgagggcag
     7501 ggtagctttc ttgggcactc ccagcgaagc cgtcgacttc ttttcctagt gagttcgatg
     7561 tgtttattaa gggtatctag tattacataa catctcaact cctatccagc gtgggtgccc
     7621 agtgtcctac caactacaat ccggcggact tttacgtaca ggtgttggcc gttgtgcccg
     7681 gacgggagat cgagtcccgt gatcggatcg ccaagatatg cgacaatttt gccattagca
     7741 aagtagcccg ggatatggag cagttgttgg ccaccaaaaa tctggagaag ccactggagc
     7801 agccggagaa tgggtacacc tacaaggcca cctggttcat gcagttccgg gcggtcctgt
     7861 ggcgatcctg gctgtcggtg ctcaaggaac cactcctcgt aaaagtgcga cttattcaga
     7921 caacggtgag tggttccagt ggaaacaaat gatataacgc ttacaattct tggaaacaaa
     7981 ttcgctagat tttagataga attgcctgat tccacaccct tcttagtttt tttcaatgag
     8041 atgtatagtt tatagttttg cagaagataa ataaatttca tttaactcgc gaatattaat
     8101 gagatgcgag taacatttta atttgcagat ggttgccatc ttgattggcc tcatcttttt
     8161 gggccaacaa ctcacgcaag tgggtgtgat gaatatcaac ggagccatct tcctcttcct
     8221 gaccaacatg acctttcaaa acgtctttgc cacgataaat gtaagtcatg tttagaatac
     8281 atttgcattt caataattta ctaactttct aatgaatcga ttcgatttag gtgttcacct
     8341 cagagctgcc agtttttatg agggaggccc gaagtcgact ttatcgctgt gacacatact
     8401 ttctgggcaa aacgattgcc gaattgccgc tttttctcac agtgccactg gtcttcacgg
     8461 cgattgccta tccgatgatc ggactgcggg ccggagtgct gcacttcttc aactgcctgg
     8521 cgctggtcac tctggtggcc aatgtgtcaa cgtccttcgg atatctaata tcctgcgcca
     8581 gctcctcgac ctcgatggcg ctgtctgtgg gtccgccggt tatcatacca ttcctgctct
     8641 ttggcggctt cttcttgaac tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc
     8701 tctcatggtt ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg gacgtggagc
     8761 cgggcgaaat tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc
     8821 tggagacgct taacttctcc gccgccgatc tgccgctgga ctacgtgggt ctggccattc
     8881 tcatcgtgag cttccgggtg ctcgcatatc tggctctaag acttcgggcc cgacgcaagg
     8941 agtagccgac atatatccga aataactgct tgtttttttt ttttaccatt attaccatcg
     9001 tgtttactgt ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc caataaaaac
     9061 aagatatgac ctatagaata caagtatttc cccttcgaac atccccacaa gtagactttg
     9121 gatttgtctt ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa aactcgttta
     9181 tcgctacata aaacaccggg atatattttt tatatacata cttttcaaat cgcgcgccct
     9241 cttcataatt cacctccacc acaccacgtt tcgtagttgc tctttcgctg tctcccaccc
     9301 gctctccgca acacattcac cttttgttcg acgaccttgg agcgactgtc gttagttccg
     9361 cgcgattcgg ttcgctcaaa tggttccgag tggttcattt cgtctcaata gaaattagta
     9421 ataaatattt gtatgtacaa tttatttgct ccaatatatt tgtatatatt tccctcacag
     9481 ctatatttat tctaatttaa tattatgact ttttaaggta attttttgtg acctgttcgg
     9541 agtgattagc gttacaattt gaactgaaag tgacatccag tgtttgttcc ttgtgtagat
     9601 gcatctcaaa aaaatggtgg gcataatagt gttgtttata tatatcaaaa ataacaacta
     9661 taataataag aatacattta atttagaaaa tgcttggatt tcactggaac taggctagca
     9721 taacttcgta taatgtatgc tatacgaagt tatgctagcg gatccaagct tgcatgcctg
     9781 caggtcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
     9841 agcggagtac tgtcctccga gcggagtact gtcctcc
//
