LOCUS       sequence-177789-        9877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5 tagged mutant yki in Drosophila..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Oh H, Irvine KD
  TITLE     In vivo analysis of Yorkie phosphorylation sites.
  JOURNAL   Oncogene. 2009 Apr 30;28(17):1916-27. doi: 10.1038/onc.2009.43. Epub
            2009 Mar 30.
  PUBMED    19330023
REFERENCE   2  (bases 1 to 9877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2009.43"; journalName: "Oncogene"; date: "2009-04-30- 
            30"; volume: "28"; issue: "17"; pages: "1916-27"
COMMENT     Sequence Label: pUAST-attB-yki:V5-S250A
FEATURES             Location/Qualifiers
     source          1..9877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..254
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     201..222
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             1638..1679
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1689..1706
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     intron          1796..1861
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1991..2011
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2283..2417
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2320..2339)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2374..2393
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    2446..2515
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(2734..2752)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2773..2789)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2773..2789)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2786..2808)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2797..2813
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2821..2827,2828..2845,2846..2851))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2866..2887
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3004..3021)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3175..3763)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3255..3274)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3934..4725,4726..4794))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4557..4576
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4795..4899)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4925..5380)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5062..5083)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5274..5293
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5507..5529
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5521..5538
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5522..5538
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5545..5564
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5545..5563
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5597..5615)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6067..6138,6540..6813,6888..7542,7604..7919,8123..8254,
                     8325..8939)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    9714..9747
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    9780..9874
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 agactctagc gagcgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa
       61 ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc
      121 tgaacaagct aaacaatctg cagtaaagtg caagttaaag tgaatcaatt aaaagtaacc
      181 agcaaccaag taaatcaact gcaactactg aaatctgcca agaagtaatt attgaataca
      241 agaagagaac tctgaatagg gaattgggaa ttcgttaaca gatctgcggc cgcggctcga
      301 gggtacctac tagtccagtg tggtggaatt ctgcagatat gtgcgcgtgc ctaatcgcta
      361 agataattct atgtagtttt cgtttgtata caataagtgc cttttatatg ttaacgacga
      421 tgtcagccag cagcaataca aacagcctga tcgagaagga gatcgacgac gaggacatgc
      481 tttcgccgat caagtccaac aacctggtgg tgcgggtcaa ccaggacacg gacgacaacc
      541 tgcaggcgct attcgacagc gtcctgaatc cgggtgacgc caagcgcccg ctgcagctgc
      601 ccctgcgcat gcggaagctg cccaactcct tcttcacgcc cccggcgccc tcgcactcgc
      661 gggccaacag cgccgactcc acctacgacg cgggctccca gtcgagcatc aacatcggga
      721 acaaggcgtc catcgtccag cagccagatg gccagtcgcc catcgccgcc atcccccagc
      781 tccagattca gccgtctccc cagcacagcc gcctggcgat acatcactcc cgagcccgca
      841 gcagccccgc ctcgctgcag cagaactaca atgtgcgcgc ccggagcgac gcagcagcag
      901 ccaacaatcc gaatgccaat ccgagcagcc aacagcagcc cgctgggccc actttcccag
      961 agaacagtgc ccaagagttc cccagcggcg ccccggccag ctcggccatt gatctggacg
     1021 ccatgaacac ctgcatgtcg caggacattc ccatgtccat gcagacagtg cacaagaagc
     1081 agcgcgccta cgacgtcatc agccccattc agttgaaccg ccaactaggc gccttgccgc
     1141 cgggatggga gcaagccaag accaatgatg gccagatcta ctacttgaat catactacaa
     1201 aatctacgca gtgggaggat cccagaatcc aatatcgcca gcagcagcaa atcttgatgg
     1261 ccgagcgaat aaagcagaat gatgttttgc aaactacaaa acaaactacc acatcgacca
     1321 ttgctaacaa tttgggtcca ctgccggatg gttgggagca ggcagttacc gagtccggag
     1381 atctttactt tataaatcac attgatcgaa cgacttcatg gaatgatccc agaatgcaat
     1441 ctgggcttag cgtgctcgac tgcccagata acttagtgtc ttccctccag attgaggata
     1501 atctttgcag taacttgttc aatgacgcac aggccattgt aaatccgccg tcttcccaca
     1561 aacctgacga tttggaatgg tataaaatta attatccagc acagtggcgg ccgctcgagt
     1621 ctagagggcc cttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc
     1681 gtaccggtca tcatcaccat caccattgag tttctagagg atctttgtga aggaacctta
     1741 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
     1801 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
     1861 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
     1921 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
     1981 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
     2041 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
     2101 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat atttgatgta
     2161 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     2221 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     2281 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     2341 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     2401 cttatcatgt ctggatccac tagtgtcgac gatgtaggtc acggtctcga agccgcggtg
     2461 cgggtgccag ggcgtgccct tgggctcccc gggcgcgtac tccacctcac ccatctggtc
     2521 catcatgatg aacgggtcga ggtggcggta gttgatcccg gcgaacgcgc ggcgcaccgg
     2581 gaagccctcg ccctcgaaac cgctgggcgc ggtggtcacg gtgagcacgg gacgtgcgac
     2641 ggcgtcggcg ggtgcggata cgcggggcag cgtcagcggg ttctcgacgg tcacggcggg
     2701 catgtcgaca ctagttctag ccagcttttg ttccctttag tgagggttaa tttcgagctt
     2761 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     2821 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     2881 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     2941 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     3001 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3061 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3121 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3181 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3241 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3301 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3361 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3421 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3481 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3541 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3601 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3661 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3721 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3781 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3841 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3901 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3961 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     4021 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4081 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4141 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     4201 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     4261 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4321 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4381 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4441 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4501 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     4561 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     4621 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     4681 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     4741 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4801 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     4861 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4921 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     4981 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     5041 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     5101 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     5161 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     5221 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     5281 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     5341 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     5401 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     5461 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     5521 tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt gggtacgtac
     5581 cgggccccta gtatgtatgt aagttaataa aacccatttt tgcggaaagt agataaaaaa
     5641 aacatttttt ttttttactg cactggatat cattgaactt atctgatcag ttttaaattt
     5701 acttcgatcc aagggtattt gatgtaccag gttctttcga ttacctctca ctcaaaatga
     5761 cattccactc aaagtcagcg ctgtttgcct ccttctctgt ccacagaaat atcgccgtct
     5821 ctttcgccgc tgcgtccgct atctctttcg ccaccgtttg tagcgttacg tagcgtcaat
     5881 gtccgccttc agttgcattt tgtcagcggt ttcgtgacga agctccaagc ggtttacgcc
     5941 atcaattaaa cacaaagtgc tgtgccaaaa ctcctctcgc ttcttatttt tgtttgtttt
     6001 ttgagtgatt ggggtggtga ttggttttgg gtgggtaagc aggggaaagt gtgaaaaatc
     6061 ccggcaatgg gccaagagga tcaggagcta ttaattcgcg gaggcagcaa acacccatct
     6121 gccgagcatc tgaacaatgt gagtagtaca tgtgcataca tcttaagttc acttgatcta
     6181 taggaactgc gattgcaaca tcaaattgtc tgcggcgtga gaactgcgac ccacaaaaat
     6241 cccaaaccgc aattgcacaa acaaatagtg acacgaaaca gattattctg gtagctgttc
     6301 tcgctatata agacaatttt tgagatcata tcatgatcaa gacatctaaa ggcattcatt
     6361 ttcgactata ttctttttta caaaaaatat aacaaccaga tattttaagc tgatcctaga
     6421 tgcacaaaaa ataaataaaa gtataaacct acttcgtagg atacttcggg gtactttttg
     6481 ttcggggtta gatgagcata acgcttgtag ttgatatttg agatccccta tcattgcagg
     6541 gtgacagcgg agcggcttcg cagagctgca ttaaccaggg cttcgggcag gccaaaaact
     6601 acggcacgct ccggccaccc agtccgccgg aggactccgg ttcagggagc ggccaactag
     6661 ccgagaacct cacctatgcc tggcacaata tggacatctt tggggcggtc aatcagccgg
     6721 gctccggatg gcggcagctg gtcaaccgga cacgcggact attctgcaac gagcgacaca
     6781 taccggcgcc caggaaacat ttgctcaaga acggtgagtt tctattcgca gtcggctgat
     6841 ctgtgtgaaa tcttaataaa gggtccaatt accaatttga aactcagttt gcggcgtggc
     6901 ctatccgggc gaacttttgg ccgtgatggg cagttccggt gccggaaaga cgaccctgct
     6961 gaatgccctt gcctttcgat cgccgcaggg catccaagta tcgccatccg ggatgcgact
     7021 gctcaatggc caacctgtgg acgccaagga gatgcaggcc aggtgcgcct atgtccagca
     7081 ggatgacctc tttatcggct ccctaacggc cagggaacac ctgattttcc aagccatggt
     7141 gcggatgcca cgacatctga cctatcggca gcgagtggcc cgcgtggatc aggtgatcca
     7201 ggagctttcg ctcagcaaat gtcagcacac gatcatcggt gtgcccggca gggtgaaagg
     7261 tctgtccggc ggagaaagga agcgtctggc attcgcctcc gaggctctaa ccgatccgcc
     7321 gcttctgatc tgcgatgagc ccacctccgg actggactcc tttaccgccc acagcgtcgt
     7381 ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc atcctgacca ttcatcagcc
     7441 gtcttccgag ctgtttgagc tctttgacaa gatccttctg atggccgagg gcagggtagc
     7501 tttcttgggc actcccagcg aagccgtcga cttcttttcc tagtgagttc gatgtgttta
     7561 ttaagggtat ctagtattac ataacatctc aactcctatc cagcgtgggt gcccagtgtc
     7621 ctaccaacta caatccggcg gacttttacg tacaggtgtt ggccgttgtg cccggacggg
     7681 agatcgagtc ccgtgatcgg atcgccaaga tatgcgacaa ttttgccatt agcaaagtag
     7741 cccgggatat ggagcagttg ttggccacca aaaatctgga gaagccactg gagcagccgg
     7801 agaatgggta cacctacaag gccacctggt tcatgcagtt ccgggcggtc ctgtggcgat
     7861 cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt gcgacttatt cagacaacgg
     7921 tgagtggttc cagtggaaac aaatgatata acgcttacaa ttcttggaaa caaattcgct
     7981 agattttaga tagaattgcc tgattccaca cccttcttag tttttttcaa tgagatgtat
     8041 agtttatagt tttgcagaag ataaataaat ttcatttaac tcgcgaatat taatgagatg
     8101 cgagtaacat tttaatttgc agatggttgc catcttgatt ggcctcatct ttttgggcca
     8161 acaactcacg caagtgggtg tgatgaatat caacggagcc atcttcctct tcctgaccaa
     8221 catgaccttt caaaacgtct ttgccacgat aaatgtaagt catgtttaga atacatttgc
     8281 atttcaataa tttactaact ttctaatgaa tcgattcgat ttaggtgttc acctcagagc
     8341 tgccagtttt tatgagggag gcccgaagtc gactttatcg ctgtgacaca tactttctgg
     8401 gcaaaacgat tgccgaattg ccgctttttc tcacagtgcc actggtcttc acggcgattg
     8461 cctatccgat gatcggactg cgggccggag tgctgcactt cttcaactgc ctggcgctgg
     8521 tcactctggt ggccaatgtg tcaacgtcct tcggatatct aatatcctgc gccagctcct
     8581 cgacctcgat ggcgctgtct gtgggtccgc cggttatcat accattcctg ctctttggcg
     8641 gcttcttctt gaactcgggc tcggtgccag tatacctcaa atggttgtcg tacctctcat
     8701 ggttccgtta cgccaacgag ggtctgctga ttaaccaatg ggcggacgtg gagccgggcg
     8761 aaattagctg cacatcgtcg aacaccacgt gccccagttc gggcaaggtc atcctggaga
     8821 cgcttaactt ctccgccgcc gatctgccgc tggactacgt gggtctggcc attctcatcg
     8881 tgagcttccg ggtgctcgca tatctggctc taagacttcg ggcccgacgc aaggagtagc
     8941 cgacatatat ccgaaataac tgcttgtttt ttttttttac cattattacc atcgtgttta
     9001 ctgtttattg ccccctcaaa aagctaatgt aattatattt gtgccaataa aaacaagata
     9061 tgacctatag aatacaagta tttccccttc gaacatcccc acaagtagac tttggatttg
     9121 tcttctaacc aaaagactta cacacctgca taccttacat caaaaactcg tttatcgcta
     9181 cataaaacac cgggatatat tttttatata catacttttc aaatcgcgcg ccctcttcat
     9241 aattcacctc caccacacca cgtttcgtag ttgctctttc gctgtctccc acccgctctc
     9301 cgcaacacat tcaccttttg ttcgacgacc ttggagcgac tgtcgttagt tccgcgcgat
     9361 tcggttcgct caaatggttc cgagtggttc atttcgtctc aatagaaatt agtaataaat
     9421 atttgtatgt acaatttatt tgctccaata tatttgtata tatttccctc acagctatat
     9481 ttattctaat ttaatattat gactttttaa ggtaattttt tgtgacctgt tcggagtgat
     9541 tagcgttaca atttgaactg aaagtgacat ccagtgtttg ttccttgtgt agatgcatct
     9601 caaaaaaatg gtgggcataa tagtgttgtt tatatatatc aaaaataaca actataataa
     9661 taagaataca tttaatttag aaaatgcttg gatttcactg gaactaggct agcataactt
     9721 cgtataatgt atgctatacg aagttatgct agcggatcca agcttgcatg cctgcaggtc
     9781 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     9841 gtactgtcct ccgagcggag tactgtcctc cgagcgg
//
