LOCUS       sequence-177792-        9877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5 tagged mutant yki in Drosophila..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Oh H, Irvine KD
  TITLE     In vivo analysis of Yorkie phosphorylation sites.
  JOURNAL   Oncogene. 2009 Apr 30;28(17):1916-27. doi: 10.1038/onc.2009.43. Epub
            2009 Mar 30.
  PUBMED    19330023
REFERENCE   2  (bases 1 to 9877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2009.43"; journalName: "Oncogene"; date: "2009-04-30- 
            30"; volume: "28"; issue: "17"; pages: "1916-27"
COMMENT     Sequence Label: pUAST-attB-yki:V5-S111A
FEATURES             Location/Qualifiers
     source          1..9877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1225..1258
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1291..1385
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        1404..1642
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     1589..1610
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3026..3067
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3077..3094
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     intron          3184..3249
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3379..3399
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3671..3805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3708..3727)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3762..3781
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3834..3903
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(4122..4140)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4161..4177)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4161..4177)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4174..4196)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4185..4201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4209..4215,4216..4233,4234..4239))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4254..4275
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4392..4409)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4563..5151)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4643..4662)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5322..6113,6114..6182))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5945..5964
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6183..6287)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6313..6768)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6450..6471)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6662..6681
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6895..6917
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6909..6926
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6910..6926
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6933..6952
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6933..6951
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6985..7003)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(7455..7526,7928..8201,8276..8930,8992..9307,9511..9642,
                     9713..9877,1..450)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 gtgctgcact tcttcaactg cctggcgctg gtcactctgg tggccaatgt gtcaacgtcc
       61 ttcggatatc taatatcctg cgccagctcc tcgacctcga tggcgctgtc tgtgggtccg
      121 ccggttatca taccattcct gctctttggc ggcttcttct tgaactcggg ctcggtgcca
      181 gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga gggtctgctg
      241 attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc gaacaccacg
      301 tgccccagtt cgggcaaggt catcctggag acgcttaact tctccgccgc cgatctgccg
      361 ctggactacg tgggtctggc cattctcatc gtgagcttcc gggtgctcgc atatctggct
      421 ctaagacttc gggcccgacg caaggagtag ccgacatata tccgaaataa ctgcttgttt
      481 ttttttttta ccattattac catcgtgttt actgtttatt gccccctcaa aaagctaatg
      541 taattatatt tgtgccaata aaaacaagat atgacctata gaatacaagt atttcccctt
      601 cgaacatccc cacaagtaga ctttggattt gtcttctaac caaaagactt acacacctgc
      661 ataccttaca tcaaaaactc gtttatcgct acataaaaca ccgggatata ttttttatat
      721 acatactttt caaatcgcgc gccctcttca taattcacct ccaccacacc acgtttcgta
      781 gttgctcttt cgctgtctcc cacccgctct ccgcaacaca ttcacctttt gttcgacgac
      841 cttggagcga ctgtcgttag ttccgcgcga ttcggttcgc tcaaatggtt ccgagtggtt
      901 catttcgtct caatagaaat tagtaataaa tatttgtatg tacaatttat ttgctccaat
      961 atatttgtat atatttccct cacagctata tttattctaa tttaatatta tgacttttta
     1021 aggtaatttt ttgtgacctg ttcggagtga ttagcgttac aatttgaact gaaagtgaca
     1081 tccagtgttt gttccttgtg tagatgcatc tcaaaaaaat ggtgggcata atagtgttgt
     1141 ttatatatat caaaaataac aactataata ataagaatac atttaattta gaaaatgctt
     1201 ggatttcact ggaactaggc tagcataact tcgtataatg tatgctatac gaagttatgc
     1261 tagcggatcc aagcttgcat gcctgcaggt cggagtactg tcctccgagc ggagtactgt
     1321 cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct
     1381 ccgagcggag actctagcga gcgccggagt ataaatagag gcgcttcgtc tacggagcga
     1441 caattcaatt caaacaagca aagtgaacac gtcgctaagc gaaagctaag caaataaaca
     1501 agcgcagctg aacaagctaa acaatctgca gtaaagtgca agttaaagtg aatcaattaa
     1561 aagtaaccag caaccaagta aatcaactgc aactactgaa atctgccaag aagtaattat
     1621 tgaatacaag aagagaactc tgaataggga attgggaatt cgttaacaga tctgcggccg
     1681 cggctcgagg gtacctacta gtccagtgtg gtggaattct gcagatatgt gcgcgtgcct
     1741 aatcgctaag ataattctat gtagttttcg tttgtataca ataagtgcct tttatatgtt
     1801 aacgacgatg tcagccagca gcaatacaaa cagcctgatc gagaaggaga tcgacgacga
     1861 ggacatgctt tcgccgatca agtccaacaa cctggtggtg cgggtcaacc aggacacgga
     1921 cgacaacctg caggcgctat tcgacagcgt cctgaatccg ggtgacgcca agcgcccgct
     1981 gcagctgccc ctgcgcatgc ggaagctgcc caactccttc ttcacgcccc cggcgccctc
     2041 gcactcgcgg gccaacgccg ccgactccac ctacgacgcg ggctcccagt cgagcatcaa
     2101 catcgggaac aaggcgtcca tcgtccagca gccagatggc cagtcgccca tcgccgccat
     2161 cccccagctc cagattcagc cgtctcccca gcacagccgc ctggcgatac atcactcccg
     2221 agcccgcagc agccccgcct cgctgcagca gaactacaat gtgcgcgccc ggagcgacgc
     2281 agcagcagcc aacaatccga atgccaatcc gagcagccaa cagcagcccg ctgggcccac
     2341 tttcccagag aacagtgccc aagagttccc cagcggcgcc ccggccagct cggccattga
     2401 tctggacgcc atgaacacct gcatgtcgca ggacattccc atgtccatgc agacagtgca
     2461 caagaagcag cgctcctacg acgtcatcag ccccattcag ttgaaccgcc aactaggcgc
     2521 cttgccgccg ggatgggagc aagccaagac caatgatggc cagatctact acttgaatca
     2581 tactacaaaa tctacgcagt gggaggatcc cagaatccaa tatcgccagc agcagcaaat
     2641 cttgatggcc gagcgaataa agcagaatga tgttttgcaa actacaaaac aaactaccac
     2701 atcgaccatt gctaacaatt tgggtccact gccggatggt tgggagcagg cagttaccga
     2761 gtccggagat ctttacttta taaatcacat tgatcgaacg acttcatgga atgatcccag
     2821 aatgcaatct gggcttagcg tgctcgactg cccagataac ttagtgtctt ccctccagat
     2881 tgaggataat ctttgcagta acttgttcaa tgacgcacag gccattgtaa atccgccgtc
     2941 ttcccacaaa cctgacgatt tggaatggta taaaattaat tatccagcac agtggcggcc
     3001 gctcgagtct agagggccct tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
     3061 ttctacgcgt accggtcatc atcaccatca ccattgagtt tctagaggat ctttgtgaag
     3121 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
     3181 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     3241 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
     3301 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
     3361 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
     3421 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
     3481 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
     3541 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
     3601 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     3661 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     3721 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     3781 caatgtatct tatcatgtct ggatccacta gtgtcgacga tgtaggtcac ggtctcgaag
     3841 ccgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc cacctcaccc
     3901 atctggtcca tcatgatgaa cgggtcgagg tggcggtagt tgatcccggc gaacgcgcgg
     3961 cgcaccggga agccctcgcc ctcgaaaccg ctgggcgcgg tggtcacggt gagcacggga
     4021 cgtgcgacgg cgtcggcggg tgcggatacg cggggcagcg tcagcgggtt ctcgacggtc
     4081 acggcgggca tgtcgacact agttctagcc agcttttgtt ccctttagtg agggttaatt
     4141 tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     4201 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     4261 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     4321 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     4381 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4441 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4501 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4561 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4621 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4681 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4741 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4801 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4861 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4921 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4981 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     5041 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     5101 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     5161 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     5221 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     5281 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     5341 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     5401 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5461 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     5521 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     5581 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     5641 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     5701 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5761 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5821 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5881 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5941 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6001 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     6061 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     6121 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6181 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6241 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     6301 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     6361 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     6421 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     6481 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     6541 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     6601 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     6661 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     6721 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat
     6781 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     6841 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     6901 cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg
     6961 gtacgtaccg ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag
     7021 ataaaaaaaa catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt
     7081 ttaaatttac ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact
     7141 caaaatgaca ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat
     7201 cgccgtctct ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta
     7261 gcgtcaatgt ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg
     7321 tttacgccat caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg
     7381 tttgtttttt gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt
     7441 gaaaaatccc ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac
     7501 acccatctgc cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac
     7561 ttgatctata ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc
     7621 acaaaaatcc caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt
     7681 agctgttctc gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg
     7741 cattcatttt cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg
     7801 atcctagatg cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt
     7861 actttttgtt cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc
     7921 attgcagggt gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc
     7981 caaaaactac ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg
     8041 ccaactagcc gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa
     8101 tcagccgggc tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga
     8161 gcgacacata ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt
     8221 cggctgatct gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc
     8281 ggcgtggcct atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg
     8341 accctgctga atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg
     8401 atgcgactgc tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat
     8461 gtccagcagg atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa
     8521 gccatggtgc ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag
     8581 gtgatccagg agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg
     8641 gtgaaaggtc tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc
     8701 gatccgccgc ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac
     8761 agcgtcgtcc aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt
     8821 catcagccgt cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc
     8881 agggtagctt tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga
     8941 tgtgtttatt aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc
     9001 ccagtgtcct accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc
     9061 cggacgggag atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag
     9121 caaagtagcc cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga
     9181 gcagccggag aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct
     9241 gtggcgatcc tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca
     9301 gacaacggtg agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca
     9361 aattcgctag attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg
     9421 agatgtatag tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta
     9481 atgagatgcg agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt
     9541 ttgggccaac aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc
     9601 ctgaccaaca tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat
     9661 acatttgcat ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac
     9721 ctcagagctg ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata
     9781 ctttctgggc aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac
     9841 ggcgattgcc tatccgatga tcggactgcg ggccgga
//
